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Chapter 2 Microscopy

Return to the bright-field or dark-field microscopy under the episcopic illumination.

1 Turn the BD field changeover lever to the “BF

(bright-field)” or “DF (dark-field)” position. (See
3.3.)

2 Turn the brightness control dial to adjust the

brightness. (See 3.2.1.)

3 Adjust the brightness with the desired ND filter in

the filter turret. (See 3.10.)

Содержание ECLIPSE MA200

Страница 1: ...Inverted Metallurgical Microscope ECLIPSE MA200 Instructions M498E 08 10 NF 1 2 4 ...

Страница 2: ......

Страница 3: ...ual are subject to change without notice Although every effort has been made to ensure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative Some of the equipment described in this manual may not be included in the set you have purchased If you intend to use any other equipment with thi...

Страница 4: ...their importance For your safety always follow the instructions marked with these symbols Symbol Description Warning Disregarding instructions marked with this symbol may lead to serious injury or death Caution Disregarding instructions marked with this symbol may lead to injury or property damage Meaning of Symbols Used on the Product Symbol Description Caution for heat This marking on the back o...

Страница 5: ...ed above Use of a non conforming power line may result in equipment malfunction failure or fire 5 Power cord Be sure to use the specified power cord for the product Using a wrong power cord may result in malfunctions or fire The product is classified as subject to Class I protection against electrical shock Make sure it is connected to an appropriate ground terminal protective earth terminal To pr...

Страница 6: ...ed Fiber Illuminator model C HGFI manual type or C HGFIE motorized type to MA200 to be used Handling the mercury lamp When using the mercury lamp you must take great care of the lamp Read the instruction manual for the light source and follow the instructions and cautions Ultraviolet light from an external light source If you use an external light source other than the specified ones and that has ...

Страница 7: ...tion with a temperature from 0 to 40 C and a relative humidity of 85 or less there should be no condensation Select a storage location with a temperature from 20 to 60 C and a relative humidity of 90 or less there should be no condensation If installed or stored in a location subject to high temperatures and humidity mold or condensation may form on the lens resulting in lowered performance and po...

Страница 8: ...o the c side and unplug the power cord from the outlet before attaching or detaching the lamphouse Never touch the glass surface of the lamp with bare hands Doing so may cause fingerprints grease etc to generate ghost images on the lamp surface reducing the illumination If you do get any fingerprints or dirt on the lamp wipe them clean Make sure the lamphouse cover is securely fitted to the lampho...

Страница 9: ... 3 2 2 Switching the Internal External brightness control 30 3 2 3 Displaying the POWER LED 31 3 3 Selecting the Microscopy Method 31 3 4 Eyepiece Tube 33 3 4 1 Selecting optical path 33 3 4 2 Adjusting the eyelevel risers 34 3 5 Adjusting the Interpupillary Distance 34 3 6 Adjusting the Diopters 35 3 7 Adjusting the Focus for focus operation 36 3 7 1 Using the coarse fine focus knob 36 3 7 2 Adju...

Страница 10: ...e slider MA2 MR 54 3 21 Using the Intermediate Magnification Unit MA2 MC 55 3 22 Using the HG Precentered Fiber Illuminator C HGFI C HGFIE 56 3 22 1 Procedure for turning on the power switch 56 3 23 Using the supporting pillar for dia Illuminator 100W MA2 DP 57 3 23 1 Procedure for turning on the power switch 57 3 23 2 Focusing and centering the condenser 58 3 23 3 Adjusting the field diaphragm fo...

Страница 11: ...4 11 Filters 82 4 12 Various Sliders Available for Attaching the Revolving Nosepiece 83 4 12 1 L DIC L DIHC slider single NR method 83 4 12 2 LV DIC LV DIHC slider Senarmont method 84 4 12 3 D DA analyzer slider D LP λ plate 84 4 13 Polarizer analyzer Unit and Fluorescent Unit 85 4 13 1 MA2 λP λ plate 85 4 14 Intermediate Magnification Unit 86 4 15 External Light Source 87 4 15 1 C HGFI C HGFIE HG...

Страница 12: ...oscopy remove the front cover to attach the desired unit for the microscopy to be performed BD field changeover lever Press BF Bright field Pull DF Dark field Refer to 3 3 Nosepiece Figure illustrates the MA2 NUI5 Quintuple sextuple and septuple revolving nosepieces may be used Refer to 3 9 The DIC slider analyzer slider etc can be attached depending on the revolving nosepiece used Objectives Refe...

Страница 13: ...l PC is in use Power switch OFF LED turns off Power switch ON Brightness control dial OFF Orange LED lights up Power switch ON Brightness control dial ON Green LED lights up Note The Power LED Message is only compatible with the lamphouse of the MA200 main body for the episcopic illumination and the HG precentered fiber illuminator C HGFIE motorized type Address display Address of the objective in...

Страница 14: ...he intermediate magnification unit Refer to 3 21 Attach this unit to change observation magnifications Connectors Refer to Figure 1 1 4 AC inlet Connects the power cord exclusive for the region of the product used optional Power switch Refer to 3 1 Set the switch to the side to turn on the power or to the c side to turn off the power When the power is turned on the POWER LED on the front display l...

Страница 15: ...it MA2 GR Grain Size Slider MA2 GR Grain Scale Slider MA2 PA Polarizing Filter Cube MA2 PA Polarizer Analyzer Unit MA2 UPA Polarizing Filter Cube with 1 4λPlate MA2 UPA Polarizer Analyzer Unit MA2 SRSH 10 Specimen Holder Other types than SRSH 10 are available General name Sample Holder Lamphouse connector LAMP DC12V50W Connects the cord on the epi illumination lamphouse 12V 50W Precentered Lamphou...

Страница 16: ...use attached When using an external light source to perform the microscopy follow the notes below The procedure on how to turn the power switch to ON Turn on the power switch on the external device in advance then turn on the MA200 main body Setting the Internal External brightness control changeover switch of the MA200 main body Sets to ON external mode Adjusting the brightness of the light sourc...

Страница 17: ...r the sensitive color polarization microscopy the MA2 λP λplate available For 6 revo MA2 UPA polarizer analyzer unit LV DIHC LV DIC slider Senarmont method For the sensitive color polarization microscopy the MA2 λP λplate available Epi fl microscopy p 22 LV LH 50PC Lamphouse or C HGFI C HGFIE HG Precentered Fiber Illuminator 5 revo 6 revo 7 revo LU EPI objectives or LU BD objective only For EPI ob...

Страница 18: ... light up the episcopic illumination lamp 1 Turn on the power switch See 3 1 1 The power LED on the front is lit 2 Be sure that the Internal External brightness control changeover switch is set to OFF internal mode See 3 2 2 3 Turn the brightness control dial to light up the lamp See 3 2 1 1 1 1 1 1 2 1 3 ...

Страница 19: ...al path and compensate color temperature See 3 10 6 Adjust the brightness roughly with the brightness control dial and the desired ND filter in the filter turret See 3 2 1 and 3 10 7 Turn the field diaphragm dial and the aperture diaphragm dial counter clockwise to the limit to fully open the field diaphragm and the aperture diaphragm for the episcopic illumination See 3 11 and 3 12 Place the samp...

Страница 20: ...agm image circumscribes the view field See 3 11 5 Turn the aperture diaphragm dial so that the aperture diaphragm image becomes 70 to 80 of the numerical aperture of the objective See 3 12 6 Adjust the brightness with the desired ND filter in the filter turret See 3 10 Note Adjusting focus It may be difficult to focus on a sample with small contrast such on a polished surface In a case like this s...

Страница 21: ... diaphragm are fully opened The dial positions do not change When the polarizer analyzer or the fl filter is placed into the optical path it is automatically excluded 3 Turn the brightness control dial to adjust the brightness of the episcopic illumination See 3 2 1 4 Adjust the brightness with the desired ND filter in the filter turret See 3 10 Return to the bright field microscopy under the epis...

Страница 22: ... 2 4 To perform the sensitive color polarization microscopy insert the λplate into the polarizer analyzer unit and place it into the optical path See 3 14 1 5 Turn the brightness control dial to adjust the brightness of the episcopic illumination See 3 2 1 6 Adjust the brightness with the desired ND filter in the filter turret See 3 10 7 Turn the polarizer rotation ring to adjust the polarization ...

Страница 23: ... DIC slider turn the prism movement knob whereas for using the LV DIHC LV DIC slider turn the polarizer rotation ring of the polarizer analyzer unit In either case the interference color changes continuously from dark color gray to sensitive red violet 6 Turn the brightness control dial to adjust the brightness of the episcopic illumination See 3 2 1 7 Adjust the brightness with the desired ND fil...

Страница 24: ...Table 2 1 To perform the epi fl microscopy using the C HGFI C HGFIE HG Precentered Fiber Illuminator as the external light source screw the compensation filter supplied with the fiber adapter into the MA2 FL fluorescent unit Refer to 4 15 1 Find the target and focus on the sample by BF DF microscopy under the episcopic illumination See Pages 16 to 17 and 19 Set the microscope for the epi fl micros...

Страница 25: ...under the episcopic illumination 1 Turn the BD field changeover lever to the BF bright field or DF dark field position See 3 3 2 Turn the brightness control dial to adjust the brightness See 3 2 1 3 Adjust the brightness with the desired ND filter in the filter turret See 3 10 ...

Страница 26: ...200 main body See 3 2 1 2 Turn on the TI PS100W power supply connected to the supporting pillar for dia Illuminator 3 Set the MA200 main body to ON The power LED on the front is lit The light intensity control dial for the TI PS100W power supply is enabled 4 Turn the light intensity control dial on the TI PS100W power supply to adjust the illumination You can change the output voltage within 1V to...

Страница 27: ...y with the light intensity control dial for the TI PS100W power supply and the desired ND filter on the pillar illuminator See 3 23 1 and 3 23 5 6 Turn the field diaphragm dial for the supporting pillar for dia Illuminator clockwise to the limit and fully open the field diaphragm for the diascopic illumination See 3 23 3 7 Turn the aperture diaphragm lever for the system condenser clockwise to the...

Страница 28: ...d See 3 8 2 Turn the coarse fine focus knobs and focus on the sample See 3 7 Adjust the diopter and the interpupillary distance 1 Adjust the interpupillary distance See 3 5 2 Adjust the diopter See 3 6 Adjust the focus again 1 Look into the eyepieces Move the stage to bring the observation target into the center of the view field using the stage movement knob for the X Y direction 2 Look into the ...

Страница 29: ... the view field Set the desired magnification and observe the sample 1 Turn the revolving nosepiece to place the objective of a desired magnification into the optical path 2 Move the aperture diaphragm lever on the system condenser to adjust the aperture diaphragm to a size of 70 to 80 of the objective N A See 3 23 4 3 Turn the field diaphragm dial for the supporting pillar for dia Illuminator unt...

Страница 30: ...ath and make a crossed Nicol s position See 3 23 8 4 To perform the sensitive color polarization microscopy place the TI DIC λplate into the optical path See 3 23 8 When using the sextuple revolving nosepiece use the D LP λplate instead of the TI DIC λplate if desired See 3 18 1 5 Turn the light intensity control dial on the TI PS 100W power supply to adjust the brightness of the diascopic illumin...

Страница 31: ...icroscope during the use of an external light source To use the HG precentered fiber illuminator or the supporting pillar for dia Illuminator 100W provided as an external light source set the power switch on the external light source to ON in advance then set the power switch on the MA200 main body to ON See 3 22 1 and 3 23 1 3 1 2 Power supply of the lamp The product has a built in power supply c...

Страница 32: ... the dial to OFF to turn off the lamp 3 2 2 Switching the Internal External brightness control Figure 3 2 2 Switching the Internal External brightness control To adjust the lamp for the lamphouse LV LH50PC with the brightness control dial be sure to set the Internal External brightness control changeover switch EXTERNAL ON OFF to OFF internal mode on the rear To adjust the brightness of the lampho...

Страница 33: ...sample or to shoot record its image set the brightness control dial to the index as shown at the 3 O clock position of the dial in the Figure 3 2 1 and place the NCB11 filter into the optical path In this condition the voltage applied to the lamp is approximately 9 V and the color reproduction is improved maximally To adjust the brightness of the illumination use ND filters See 3 10 3 3 Selecting ...

Страница 34: ... available For 6 revo MA2 UPA polarizer analyzer unit LV DIHC LV DIC slider Senarmont method For the sensitive color polarization microscopy MA2 λP λ plate available BF Press Set bright field microscopy under the episcopic illumination And then attach polarizer analyzer unit to make crossed Nicol s position Place DIC prism into optical path to perform DIC microscopy To adjust the contrast turn the...

Страница 35: ...e 3 4 1 Selecting optical path Light distribution Lever position Binocular tube Vertical tube IN Press 100 0 OUT Pull 0 100 The light distribution for the eyepiece tube part and the back port can be selected with the eyepiece tube back port changeover lever Figure 3 4 2 Selecting optical path Light distribution Lever position Eyepiece tube Back port IN Press 100 0 OUT Pull 55 45 Optical path chang...

Страница 36: ...tance Adjust the interpupillary distance or the distance between the eyepieces according to user s eye distance Figure 3 5 1 Adjusting the interpupillary distance Before adjusting the interpupillary distance follow the procedure described in 2 1 Bright field Microscopy under the Episcopic Illumination or 2 6 Bright field Microscopy under the Diascopic Illumination and focus on the sample with the ...

Страница 37: ...2 Follow the procedure described in 2 1 Bright field Microscopy under the Episcopic Illumination or 2 6 Bright field Microscopy under the Diascopic Illumination and focus the sample with the 10X objective 3 Place the 50X objective into the optical path Turn the coarse fine focus knobs to focus on the sample 4 Place the 5X or 10X objective into the optical path 5 Look into the right eyepiece with y...

Страница 38: ...able range of the revolving nosepiece is 4 mm 2 mm top datum surface for stage To raise the revolving nosepiece turn the knob forward Figure 3 7 1 Relationship between focus knob rotation and revolving nosepiece vertical movement Do not attempt following operations because doing so may cause the product failure Turning the left and right knobs in opposite directions at the same time Keep turning t...

Страница 39: ...mple from tipping over or being moved The sample holder is supplied with a rotation knob If you turn the ring holding the rotation knob you can turn the sample with the observation position unmoved Various options are available for the sample holder as shown below Select an appropriate option according to the size or observation method of the sample used Item Model Remarks Sample holder Standard H...

Страница 40: ...egrees This offers easy operation and user convenience for switching between the focus knob and the stage knob The stage is equipped with the graduations that move 50 mm max to X or Y direction Figure 3 8 2 Changing the observation position A cross roller guide is provided to move the stage in the X Y direction The Y direction movement rack is located at the bottom of the stage Do not touch the ra...

Страница 41: ...bjectives Turn the revolving nosepiece to the click stop position by hand to switch the magnification for objectives The observing area may be out of the optical path hole if the observation position is adjusted by moving the stage Do not turn the revolving nosepiece under such condition or the objective may touch the stage When turning the revolving nosepiece be sure that the objective does not t...

Страница 42: ...duces the light intensity to 1 16 Transmittance approx 6 3 ND4 Neutral Density filter ND4 Adjusts the brightness for general microscopy or photomicroscopy Reduces the light intensity to 1 4 Transmittance approx 25 Optional USER Attach as desired Turret 1 on user side Dummy OPEN Without filters GIF Green Interference filter GIF Improves the contrast for microscopy under a monochrome light or when p...

Страница 43: ... adjustment of the field diaphragm opening should be performed after centering the diaphragm Centering procedure for the field diaphragm Figure 3 11 2 Centering the field diaphragm 1 Follow the procedure described in 2 1 Bright field Microscopy under the Episcopic Illumination and focus on the sample with the 10X objective 2 Stop down the field diaphragm by turning the field diaphragm dial 3 Inser...

Страница 44: ...out 70 to 80 of the numerical aperture of the objective When the reflectance of the sample is low the diaphragm image may not be seen In this case change to a sample of a near polished surface Centering procedure for the aperture diaphragm Figure 3 12 2 Centering the aperture diaphragm 1 Follow the procedure described in 2 1 Bright field Microscopy under the Episcopic Illumination and focus on the...

Страница 45: ... 14 A single push or pull of the unit allows you to insert or remove both the polarizer and analyzer from the optical path at the same time The MA2 UPA is equipped with the 1 4 λ plate If using the MA2 UPA for polarization observation the low contrast sample such as aluminum can be observed with clear contrast For the simplified polarization microscopy under the diascopic illumination only analyze...

Страница 46: ... s position Place the unit in the optical path And then place a sample with a flat and plain surface onto the stage Set up the microscope for the simplified polarization microscopy under the episcopic illumination Remove one eyepiece from the eyepiece tube and look inside the open sleeve You can see the objective pupil as a bright circle Turn the polarizer rotation ring in either direction until a...

Страница 47: ...the diascopic illumination 3 14 1 Inserting removing the λ plate Figure 3 14 2 Inserting removing the λ plate Push the λ plate slider into the polarizer analyzer unit placed in the optical path When it hits the second click stop position the λ plate enters the optical path Pull out the λ plate slider and the λ plate is removed from the optical path when it hits the first click stop position Second...

Страница 48: ...ts the second click stop position the DIC prism enters the optical path Pull out the DIC slider When it hits the first click stop position the DIC prism is removed from the optical path 3 15 2 Setting the DIC prism Figure 3 15 3 DIC prism position indication For the DIC microscopy switch the DIC prism depending on the objective to be used The industrial LU objective has a character A or B showing ...

Страница 49: ... under the episcopic microscopy in Senarmont method The LC DIHC is designed for high contrast observation 3 16 1 Selecting the DIC slider Both the LV DIHC and the LV DIC sliders have A and B types to be selected according to the objective The industrial LU objective has a character A or B showing the DIC prism position which is indicated next to the magnification N A value on the objective barrel ...

Страница 50: ...on The D DA analyzer is not required when the polarizer analyzer unit MA2 PA MA UPA is attached to the microscope during the simplified polarization microscopy under the diascopic illumination 3 17 1 Inserting removing the analyzer from the optical path Figure 3 17 2 Inserting removing the analyzer from the optical path Two slots are provided on the front of the revolving nosepiece Insert the D DA...

Страница 51: ...om the optical path Figure 3 18 2 Inserting removing the λ plate from the optical path Two slots are provided on the front of the revolving nosepiece Insert the D LP analyzer slider with the nameplate facing down into the slot with λ indicated near the objective When it hits the second click stop position the λ plate enters the optical path Pull out the slider and when it hits the first click stop...

Страница 52: ...uorescent unit Refer to 4 15 1 Fluorescent unit Characteristics of EX filter nm Characteristics of BA filter nm MA2 FL G 510 560 590 MA2 FL B 450 490 520 MA2 FL BV 400 440 470 MA2 FL V 380 420 450 3 19 1 Inserting removing the fl filter from the optical path Figure 3 19 2 Inserting removing the fluorescent unit from the optical path Push in the unit attached on the microscope When it hits the seco...

Страница 53: ...sample In this case the image becomes bright but the amount of autofluorescence becomes large and fading of the sample occurs soon On the contrary when the filter has a narrow bandwidth a small amount of excitation lights will be irradiated on the sample In this case the image becomes dark but the amount of autofluorescence becomes small and fading of the sample occurs late For samples with pronou...

Страница 54: ...bsorbs the excitation light energy As a result fluorescent lights are emitted from the fluorophore instead When a sample is labeled with a fluorophore that emits fluorescent lights of very close wavelengths to the excitation light select a barrier filter with the shortest cut on wavelength permitted by performance requirements for efficient fluorescent microscopy A longer cut on wavelength tends t...

Страница 55: ...to use the 5X objective select the reticle number two scales down Lattice scale 0 5 mm intervals Vertical Horizontal 20 each The lattice scale is normally used for the Nonmetallic Inclusion test and it can also be used for simple lattice measurement The lattice whose intervals on a reticle indicate 0 5 mm consists of 20 vertical or horizontal lines When the 10X objective is used the lattice interv...

Страница 56: ... inserted the scale slider is pressed down by a leaf spring built inside the slot to adjust the desired inserting position Adjust its position to locate the reticle on the right hand side of the view field When inserting the slider into the slot face its side with the indication toward the front of the microscope To capture the scale using the camera adjust the desired inserting position by checki...

Страница 57: ...fication unit to the MA200 main body to change the observation magnification for the binocular vertical tube or the back port Turn the turret for this unit to select the desired magnification from the magnifications 1X dummy 1 5X and 2X Set the turret at the desired magnification displayed on the turret click stop Magnification Magnification changeover turret ...

Страница 58: ...n the fiber illuminator lights up the LAMP indicator starts blinking Once the illuminator starts up the LAMP indicator stops blinking and lights up If the C HGFIE motorized fiber illuminator is in use the communication function on the illuminator does not activate until it starts Be sure to wait until the illuminator starts up 3 Set the POWER switch to the side to turn on the power on the MA200 ma...

Страница 59: ...for dia Illuminator is properly connected to the power supply and the brightness control dial on MA200 main body is set to OFF 2 Set the POWER switch to the side on the power supply The POWER indicator on the power supply and the lamp light up 3 Set the POWER switch to the side to turn on the MA200 main body The power LED on the front lights up 4 To turn off the pillar illuminator set the power sw...

Страница 60: ...age comes into the view field 3 Turn the condenser focus knob of the supporting pillar for dia Illuminator to focus on the field diaphragm image 4 Turn the condenser centering screws 2 for both sides for the supporting pillar for dia Illuminator to move the field diaphragm image to the center of the view field 5 Place the 50X objective into the optical path 6 Adjust the size of the field diaphragm...

Страница 61: ...for the condenser lens Move the field diaphragm image into the center of view field Adjust its size to match the view field Figure 3 23 4 Adjusting the field diaphragm 1 Place the 10X objective into the optical path 2 Stop down the field diaphragm by turning the field diaphragm dial on the supporting pillar for dia Illuminator until the field diaphragm image comes into the view field 3 Turn the co...

Страница 62: ... increased while the contrast and the depth of focus are reduced The resolution the brightness the contrast and the depth of focus are related to one another and cannot be changed independently Set the conditions to fit the requirements of the sample and the intended application Adjust the aperture diaphragm while looking at the actual diaphragm image Turn the aperture diaphragm lever counter cloc...

Страница 63: ...he optical path and the lamp voltage is set to the same display as the lamp rating Remove from optical path when performing monochrome imaging 3 23 6 Condenser refocusing clamp Figure 3 23 7 Condenser refocusing clamp Form the field diaphragm image on the sample surface by turning the condenser focus knob Turn the condenser refocusing clamp clockwise to the limit to mark this position When the con...

Страница 64: ...X objective and focus on the sample 3 Move the stage to remove the sample out of the view field In its place bring an almost dust free area into the view field There must be no dust in the whole view field 4 Remove an eyepiece from the binocular part to attach the centering telescope To observe the objective pupil turn the eyepiece while pressing the flange on the centering telescope 5 Place the a...

Страница 65: ...ser mount rotation clamp screw Hold the condenser turret and turn both the condenser and the polarizer at the same time Tighten the mount rotation clamp screw to fix it at the position where a dark cross appears in the objective pupil Be careful not to accidentally loosen the mount rotation clamp screw during microscopy Mount rotation clamp screw ...

Страница 66: ...the supporting arm Figure 3 24 1 Supporting arm The rotation torque can be adjusted depending on the tightness of the docking section of the mount arm to keep the DS L2 steady while achieving more comfortable operation for angle adjustment Turn the two nuts on the docking sections with the supplied tool and control each rotation torque for the horizontal vertical direction For using the DS L2 refe...

Страница 67: ...buttons Closes the microscope info menu Resizes the microscope info menu Displays the objective info Refer to 3 25 2 To display the objective lens info on the DS L2 use the MA200 Setup in MA200 Setup Tool supplied with MA200 to allow the user to register the info from an external PC to the microscope For installing and operating MA200 Setup refer to the software manual of MA200 Setup Tool Figure 3...

Страница 68: ...croscope Once you calibrate the reference length you can have a simple means of measuring distances of an image on DS L2 When MA200 is connected with the DS L2 you can save calibration values for each nosepiece address in order to measure the length of a desired image Additionally when the intermediate magnification unit is attached to MA200 the magnification value of the intermediate magnificatio...

Страница 69: ... microscope function can be set to ON OFF for each camera head Figure 3 25 3 Registering the measurement interlock If you check the checkbox the measurement corresponds to the optical magnification of the microscope for the displayed image captured by the camera connected to the selected camera port of the DS SW For capturing the image and setting the camera refer to the instruction manual supplie...

Страница 70: ... the microscope Hexagonal wrench 5 mm Filter replacing tool supplied with the microscope When these tools are not used place them in the tool holder at the right end of the top surface of the product Installation location This product is a precision optical instrument The usage or storage in an inappropriate environment may result in malfunctions or poor performance Consider the following factors ...

Страница 71: ...H50PC Lamp Nikon 12V 50W longlife halogen lamp model name LV HL50W or 12V 50W shortlife halogen lamp from other manufacturers model name OSRAM HLX 64610 OSRAM HLX 64611 or PHILIPS 7027 For the diascopic illumination Lamphouse Nikon 12V 100W Precentered Lamphouse LC model name D LH LC Lamp Nikon 12V 100W halogen lamp model name OSRAM HLX 64623 or PHILIPS 77241 TI PS 100W power supply If you wish to...

Страница 72: ...nator for the episcopic illumination C LHGFI HG lamp MA2 GR grain scale slider MA2 MR scale slider MA2 PA MA2 UPA polarizer analyzer unit MA2 λP λ plate MA2 FL fluorescent Unit G B BV V MA2 MC intermediate magnification unit C ER eyelevel riser ELWD S condenser TI C system condenser T DIC λ plate T P2 polarizer Filter GIF NCB11 ND2 ND16 MA2 TI3 trinocular eyepiece Y TB binocular eyepiece tube C mo...

Страница 73: ...orting pillar for dia Illuminator 100W under diascopic illumination MA2 TI3 trinocular eyepiece tube Eyepiece tube Y TB binocular eyepiece tube Eyepiece All types LV NU5A motorized nosepiece MA2 NUI5 revolving nosepiece D ND6 revolving nosepiece Revolving nosepiece D NI7 revolving nosepiece c 5 revo c 5 revo 6 revo CFI LU Plan FLUOR EPI CFI L Plan EPI Objective CFI LU Plan FLUOR BD 1 2 c LU BD obj...

Страница 74: ... 6 revo 7 revo 3 The CFI L Plan EPI objective lens cannot be used for DIC microscopy under the epi illumination epi fl microscopy 4 D DA analyzer is attachable for 6 revo only 5 D LP λ plate is attachable for 6 revo only 6 L DIC L DIHC slider is attachable for 5 revo only 7 LV DIC LV DIHC slider is attachable for 6 revo only ...

Страница 75: ...rting pillar for dia Illuminator 100W D LH LC Lamphouse Power cord Power cord Power cord Power cord PC DS L2 AC adapter AC adapter Power cord Intermediate magnification unit MA200 main body Nosepiece Controller 2 Note 2 RS232C cross cable Signal cable USB cable Fiber adapter HG fiber C HGFI HG Precentered Fiber Illuminator C HGFIE HG Precentered Fiber Illuminator LV LH50PC Lamphouse Power supply A...

Страница 76: ...an be controlled on a PC or the DS L2 connected to MA200 via the USB cable The indicates external control status display is unavailable To control MA200 on a PC or to display the current status of the MA200 on a PC use the SDK in the supplied MA200 Setup Tool software For details on how to install the SDK on a PC refer to the software manual supplied with the MA200 Setup Tool ...

Страница 77: ... source MA2 DP supporting pillar for dia Illuminator 100W To use the MA2 DP for the diascopic illumination set the brightness control dial to OFF LV NU5A motorized nosepiece Rotation of the revolving nosepiece 3 Address for the revolving nosepiece Rotation of the revolving nosepiece 3 Address for the revolving nosepiece Connect the Motorized Nosepiece Controller 2 with the MA200 main body using th...

Страница 78: ...he power switch on the Nosepiece Controller 2 to the side to turn it on 2 Set the power switch on the MA200 main body to the side to turn it on For using the C HGFIE HG Precentered Fiber Illuminator motorized type and the Nosepiece Controller 2 1 Set the power switch on the fiber illuminator to the side and wait until it starts up 2 Set the power switch on the Nosepiece Controller 2 to the side to...

Страница 79: ...ing the illumination If you do get any fingerprints or dirt on the lamp wipe them clean Make sure the lamphouse cover is securely fitted to the lamphouse after replacing lamps Never turn on the lamp with the lamphouse cover removed When you dispose of the replaced lamp do not break it Instead dispose of the used lamp as industrial waste or dispose of it according to the local regulations and rules...

Страница 80: ...ch the microscope For using the MA2 NUI5 D ND6 or D NI7 manual revolving nosepiece be sure to provide a cable connection between the revolving nosepiece and the microscope For using the LV NU5A motorized nosepiece be sure to provide a cable connection for the LV NCNT2 Nosepiece Controller 2 4 5 1 Attaching the nosepiece 1 Fully loosen the nosepiece clamp screw located on the front of the mount usi...

Страница 81: ...via cable connection Additionally the address information can be output to a device including a PC or the DS L2 via USB connection 4 5 3 Connecting the motorized nosepiece and the Nosepiece Controller 2 Make sure to turn off the motorized Nosepiece Controller 2 to attach or remove the signal cable 1 Loosen the rear panel clamp screw on the microscope with the supplied hexagonal screwdriver to remo...

Страница 82: ...crew holes on the stage with the corresponding screw holes on the mount part 2 Insert three hexagonal socket head bolts M6 x 20 mm and secure them with the supplied hexagonal wrench 5 mm Attach the stage so that its top surface is located in a horizontal position 4 6 1 Sample holder The MA2 SR rectangular stage is supplied with a sample holder outer diameter φ108 mm Teardrop shaped aperture φ22 mm...

Страница 83: ...nosepiece in advance 4 8 Eyepiece Tube Fully loosen the eyepiece tube clamp screw on the upper left with the supplied hexagonal screwdriver Insert the dovetail on the eyepiece tube into the circular groove on the attaching part to secure the eyepiece tube with clamp screw Hold the eyepiece tube by hand when loosening the clamp screw to prevent a sudden disconnection and falling 4 9 Eyepieces Attac...

Страница 84: ...he glass with a soft blower or brush 4 11 Filters You can attach the φ25 mm up to 3 mm thick filter on the optional slot for the filter turret 1 or 2 When handling filters put on gloves and do not touch the surface of filters with bare hands And be careful not to let dust or fingerprints get on them 1 Loosen the clamp screws 2 for the filter turret with the supplied hexagonal screwdriver 2 Remove ...

Страница 85: ... or LV NU5A motorized nosepiece 1 Loosen the DIC slider limit screw on the nosepiece using a hexagonal screwdriver 2 Remove the slot from the revolving nosepiece to insert the DIC slider 3 Screw in the DIC slider limit screw When removing the DIC slider from the nosepiece fully loosen the DIC slider limit screw using a hexagon screwdriver and then pull out the slider Sliding limit groove Prism set...

Страница 86: ... revolving nosepiece Two slots are provided for the revolving nosepiece Insert the analyzer slider into the slot indicated with A far from the objective and insert the λ plate into the slot indicated with λ near the objective Insert these two sliders with their nameplates down When the sliders hit the second click stop position the analyzer or D LP λ plate enters the optical path Pull them out to ...

Страница 87: ...click stop position the polarizer analyzer or the fl filter can be removed from the optical path 3 Remove the dust protection cover from the front cover to hide the operation port then return the front cover to the original place to fix it with the bolt To perform the epi fl microscopy using the C HGFI C HGFIE HG Precentered Fiber Illuminator as the external light source screw the compensation fil...

Страница 88: ...r inside the opening as the figure shows Remove the cable from the cable keeper then connect the cable to the connector on the intermediate magnification unit The cable keeper is attached to MA200 with a double side tape Be sure to remove the keeper before attaching the intermediate magnification unit 3 Insert the intermediate magnification unit to the opening with the MAGNIFICATION indications up...

Страница 89: ...connector on the microscope using the RS232C cross cable supplied with the C HGFIE If you fail to make this connection a fire of light may enter in the view field at the rotation of the motorized nosepiece 1 If mounted remove the LV LH 50PC Lamphouse 2 Fully loosen the fiber adapter clamp screw using the hexagonal screwdriver 3 Mount the fiber adapter onto the attaching part of the LV LH 50PC Push...

Страница 90: ...ng pillar for dia Illuminator onto the microscope Align the pins 2 on the lower surface of the supporting pillar for dia Illuminator with the holes on the attaching part Using the supplied hexagonal wrench 5 mm secure the dia pillar illuminator by tightening the four hexagonal socket head bolts M6 x 20 mm Attach the condenser mount to the supporting pillar for dia Illuminator 1 Remove the fall sto...

Страница 91: ...ndenser mount rotation clamp screw Attaching the TI C system condenser 1 Turn the condenser turret to the A position dummy hole cassette position for bright field microscopy 2 With the A label facing the front towards the user insert the dovetail on the system condenser into the bottom of the condenser holder and secure it by tightening the condenser clamp screw Attach the condenser turret by slid...

Страница 92: ...th the groove on the cylinder of the lamphouse 2 Insert a hexagonal screwdriver into the hole on the top of the supporting pillar for dia Illuminator Secure the lamphouse by tightening the lamphouse clamp screw 3 Secure the lamphouse cable with the cable clamp on the back of the supporting pillar for dia Illuminator The cable clamp is attached to the supporting pillar for dia Illuminator with the ...

Страница 93: ...er replacing the lamp Never turn on the lamp with the cover removed When you dispose of the replaced lamp do not break it Instead dispose of the used lamp as industrial waste or dispose of it according to the local regulations and rules 1 Insert a hexagonal wrench or a hexagonal screwdriver into the hole on the top of the lamphouse cover Loosen the clamp screw and remove the lamphouse cover 2 Pres...

Страница 94: ...h the filter into the slot You can insert up to four filter sliders You can insert filter sliders from the right or the left If they are all inserted from the same direction they will be difficult to handle so you should insert them alternately from the left and right Connect the TI PS100W power supply Connect the cable of the 100W diascopic illumination lamp to the 12 VDC output connector on the ...

Страница 95: ...te Be sure that the steel ball on the tip of the clamp screw is in the groove on the rotating section Tighten the rotation clamp screw to secure the rotating section 4 Use a hexagonal screwdriver to loosen the condenser mount rotation clamp screw on the left side of the condenser holder Turn the entire polarizer to align the white index mark set in Step 3 with the white index mark on the condenser...

Страница 96: ...e of the microscope 2 Align the pins 2 on the lower surface of the supporting arm with the holes on the attaching part Using the supplied hexagonal wrench 5 mm secure the supporting arm by tightening the four hexagonal socket head bolts M6 x 20 mm The standard descriptions on the DS L2 attaching part is shown as follows VESA 75 mm standard compliance UL1678 compatible Attachment screw holes M4 dep...

Страница 97: ...knob 6 The rotation torque can be adjusted depending on the tightness of the docking section of the mount arm to keep the DS L2 steady while achieving more comfortable operation for angle adjustment Turn the two nuts on the docking sections with the supplied tool and control each rotation torque for the horizontal vertical direction Cable connection between the DS L2 and MA200 1 Connect the DS cam...

Страница 98: ...taching part for the eyepiece tube then fitting the dovetail on the eyelevel riser to the circular groove of the attaching part 2 Secure the eyelevel riser by tightening the eyepiece tube clamp screw 3 Attach the eyepiece tube on the eyelevel riser and secure the eyepiece tube with clamp screw Eyepiece tube clamp screw Eyepiece tube clamp screw ...

Страница 99: ...cure it by tightening the clamp screw CAUTION After mounting the camera be sure to place cables into the cable keeper If a cable comes into contact with the lamphouse the cable sheath may melt resulting in an electrical shock or fire 4 19 Power Cord WARNING Make sure to use the specified power cord Using a wrong power cord may result in malfunctions or fire This product is classified as subject to...

Страница 100: ...nosepiece correctly p 78 The rotation of the revolving nosepiece is stopped at an incorrect position No objective is placed in the optical path Turn the revolving nosepiece to the click stop position Place the objective into the optical path p 39 The view field is invisible vignetted or uneven in brightness The sample holder is placed into the optical path Remove it from the optical path p 38 The ...

Страница 101: ...e p 50 Diascopic microscopy The condenser position is too low Adjust the condenser focus knob so that the field diaphragm image is focused on the sample surface p 59 The revolving nosepiece is not attached correctly Install the revolving nosepiece correctly p 78 The revolving nosepiece is not placed to a click stop position The objective is not placed in the optical path Turn the revolving nosepie...

Страница 102: ...ample holder correctly p 80 The interpupillary distance is not adjusted Adjust the interpupillary distance p 34 When viewing through the binocular eyepiece the image does not resolve into a single image The eyepiece diopters are not adjusted Adjust the diopters p 35 The interpupillary distance is not adjusted Adjust the interpupillary distance p 34 The eyepiece diopters are not adjusted Adjust the...

Страница 103: ...e power cord is not connected securely Connect the power cord correctly p 97 No lamp is attached Attach a lamp p 78 The lamp is blown Replace the lamp with a new one p 78 The lamp does not light even though the power switch is turned on A wrong lamp is used Use the specified lamp Refer to 7 Specifications The lamp is about to blow Replace the lamp with a new one p 78 The power cord or the cable of...

Страница 104: ...rinted Parts Cleaning tool and supplies consumables Cleaning tool Brush with soft bristles Use a cleanroom wiper in a cleanroom Cleaning supplies consumables Ethyl or methyl alcohol Lens tissue Use a cleanroom wiper in a cleanroom 6 1 Cleaning the Lenses and Filters Do not let dust fingerprint etc get on the lenses and filters Dirt on the lenses filters etc will adversely affect the view of image ...

Страница 105: ...ut the dust proof cover over this product to protect it from dust Before putting on the dust proof cover turn off the power switch of the product set the switch to the Ο side and wait until the lamphouse gets cool sufficiently 6 4 Regular Inspections fee charged Periodical inspections of this product are recommended in order to maintain peak performance Contact your nearest Nikon representative fo...

Страница 106: ...pply Power supply specification Input ratings 100 240 VAC 10 1 8 A 50 60 Hz Built in fuse ratings 250V T4A Output ratings 12 VDC 100W Maximum output current 8 4 A Electric shock protection class Class I Remarks UL listed product GS approved Illumination Köhler illumination Microscopy method Bright field Simplified polarization Filter ND2 ND16 GIF and NCB11 Lamp ratings 12 VDC 100W halogen lamp Spe...

Страница 107: ...ided Port Eyepiece tube Back 100 0 Eyepiece tube Back 55 45 Intermediate magnification Intermediate magnification unit MA2 MC attaching part provided Applicable standard This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the ...

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