Nikon ECLIPSE MA200, Инструкция по эксплуатации

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Chapter 3 Operation Details
50
3.19 Using the Fluorescent Unit (MA2-FL)
Attach the fluorescent unit to the MA200 main body to
perform the epi-fl microscopy.
A fluorescent unit consists of two types of optical
components: an excitation light filter (EX filter), a
barrier filter (BA filter). You can use the four types of
combination, "G", "B", "BV" or "V" as shown in the
table below. Select the fluorescent unit suitable for
your purpose and sample.
Figure 3.19-1 Fluorescent unit
To perform epi-fl microscopy using the
C-HGFI/C-HGFIE HG Precentered Fiber
Illuminator as the external light source, screw the
compensation filter supplied with the fiber
adapter into the filter attachment of the
fluorescent unit. (Refer to 4.15.1.)
Fluorescent unit Characteristics of EX filter (nm) Characteristics of BA filter (nm)
MA2-FL G 510-560 590
MA2-FL B 450-490 520
MA2-FL BV 400-440 470
MA2-FL V 380-420 450
3.19.1 Inserting/removing the fl filter from the optical path
Figure 3.19-2 Inserting/removing the fluorescent
unit from the optical path
Push in the unit attached on the microscope. When it
hits the second click-stop position, the fl filter enters
the optical path.
Pull out the unit. When it hits the first click-stop
position, the fl filter is removed from the optical path.
Under this condition, the compensation filter attached
to the fluorescent unit enters the optical path to
reduce glare in the bright-field observation. (Refer to
4.15.1.)
Fluorescent unit
Second click-stop position
First click-stop position
Excitation
light filter
Barrier filter
Compensation filter
attachment