65
Type B2 Exhaust HEPA Filter Leak Test
During this test the exhaust HEPA filter will be challenged and a downstream average
utilized to check for leaks. This section describes how to test the exhaust HEPA filter
on a Type B2 biosafety cabinet. The aerosol generator and photometer should be
prepared at this point as described in the preparation steps listed in the previous
section.
1. Locate a sampling port downstream of the exhaust HEPA filter. The sampling
port is not part of the biosafety cabinet. It must be facility supplied as part of the
facility’s exhaust system ducting. The sampling port should ideally be at least 10
duct diameters downstream of the exhaust HEPA filter, and the same distance
from any elbows in the duct work.
2. Connect the sampling port to the photometer.
3.
Set the photometer sampling valve to “DOWNSTREAM”. Ensure proper vacuum
at the sampling tube.
4. Open the appropriate number of Laskin nozzles on the aerosol generator (see
Table 10-3).
5. Observe the average concentration of aerosol downstream.
Acceptance Criteria
Aerosol penetration shall not exceed 0.005%.
Reinstall the work surface.
Type B2
Cabinet Width
Sash
Height
(inches)
Exhaust Air
Displacement
(CFM)
Laskin
Nozzles
Required
Calculated
Concentration
(ug/L)
1
Actual
Concentration
(ug/L)
2
4-foot
8
723
2
38
21
6-foot
8
1083
2
25
17
1. The calculated concentration was established using the formula:
Concentration (ug/l) = (# Laskin nozzles @ 23 PSIG x 13,500) / Volume of air displaced
2. Based on Mineral oil
Table 10-3
Содержание Logic+ A2
Страница 148: ...148 Wiring Diagrams The wiring diagram is also located on the blower plenum cover behind the dress panel 100 115V...
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Страница 156: ...156 Contamination in the work area...
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