42
5.
Wipe off the separation bed with a moist, lint-free cloth to
remove dust or particles. It is also advisable to wipe off the
electrodes gently with a cloth that does not leave dust or other
particles.
Note:
In order to obtain the best results possible we recommend
frequent cleaning of the electrodes. Even minor amounts of
deposited impurities have been shown to sometimes affect the
resolution and band pattern. See also Maintenance, chapter 7.
Fig. 18.
Fitting the IEF gel cover.
Positioning the gels
1.
Place a drop of water or insulating fluid ( approximately 60-75
µl) onto the middle of the gel area(s) outlined by the red lines
on the separation bed.
2.
Take one or two gels from the refrigerator. Use a pair of scissors
to cut the package along three sides. Make sure that the thin
plastic film on the gel does not stick to the package inside.
Remove the gel from its package with a pair of forceps; use the
plastic tab of the gel backing as a handle.
The thin plastic film on the gel surface protects the gel from
contaminants and from drying, and should be left on for now.
3.
Use a waterproof pen to mark the underside of the gel for
identification. You might have to wipe the back of the gel first.
5. Operation
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