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Fig. 10. The approximate molecular weight separation ranges of PhastGel gradient
media are superimposed on a histogram showing the molecular weight distribution of
native proteins. The histogram is made up of data collected from 530 proteins. Each
bar spans 10,000 daltons. (Gianazza, E., Righetti, P.G., J. Chromatography 193
(1980) 1-8). By kind permission of the authors and publisher.
PhastGel buffer strips
PhastGel buffer strips are made of high quality agarose which has
been countercharged and therefore has a low electroendosmosis
(Agarose IEF). The agarose is melted together with buffer and then
cast in the moulds. The PhastGel buffer strip holder holds buffer
strips in place on the gel. Two buffer strips are used for each gel; one
at the cathode, one at the anode. The electrodes rest on the strips
during electrophoresis and transfer current and voltage to the gel.
PhastGel buffer strips are individually sealed in airtight packages.
Once the buffer strips are removed from the package, they must be
used immediately.
PhastGel Blue R
PhastGel Blue R is a Coomassie R 350 stain stamped into convenient
tablet form. The tablets are first dissolved in water. Methanol is then
added and this solution is filtered and stored as a stock solution.
Before use, acetic acid is added. The final solution is only stable for
about one day.
One pack of PhastGel Blue R contains 40 tablets. Each tablet makes
400 ml of 0.1% stain solution. Instructions for storage and use are
included with every pack.
Optimized development methods using PhastGel Blue R are described
in chapter 9, Development technique file No. 200 and 201.
3. Description of the system