GE PhastSystem, Руководство пользователя

Страница: 16 / 106

16
through a hole in the bottom of the chamber. Similarly, by creating
excess pressure in the chamber, liquid is pushed out through the same
hole in the bottom.
Development methods
Nine development methods are available for programming. For each
method, you can program up to 20 steps. For each step, the in-port
for filling, the out-port for emptying, the duration of the step in
minutes, and the temperature for processing the gel (the chamber can
heat solutions up to 50°C) is programmed.
As programming options, each development method can have a
temperature compensation curve, and an extra alarm (to sound at a
set time during the run). More information about temperature
compensation is given in Development procedures, section 5.3.
Once the bottles of development solution are connected to the ports
(by the PVC tubing), the gels are inserted into the gel holder, the lid is
closed, the start button is pressed, and the rest is automatic. The
method ends when it reaches an empty (unprogrammed) step.
Fig. 6. Development chamber.
3. Description of the system