Biotek Synergy HT, Руководство оператора

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Dispense Module Tests
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105
Synergy HT Operator's Manual
Test Setup: Gen5™
To perform the Dispense Accuracy and Precision Test using Gen5, you’ll need to create
two
protocols containing the necessary Dispense and Read steps (one protocol per
dispenser). You can create these protocols once and then reference them in new
experiments each time you run the test. See page 110 for instructions for creating
these protocols.
™
For your convenience, we’ve included a worksheet at the end of this
chapter for recording the dispense weights, Delta OD values, calcula-
tions, and pass/fail. Make two copies of this worksheet—one for each
dispenser tested.
™
Tip : You can take advantage of Gen5’s Power Export feature to create
Microsoft Excel spreadsheets for performing the Results Analysis
calculations. See the Gen5 Help System for information on the Power
Export Builder.
Test Setup: KC4™
•
Using KC4, create three Dispense protocols for the Synergy HT. If you are
using a BioTek absorbance reader, create two additional Read protocols. See
page 115 for instructions.
•
If you are not using a BioTek absorbance reader, prepare your reader to
perform two reads with the following characteristics:
80 μ l Read 20 & 5 μ l Read
Primary Wavelength 405 nm 630 nm
Reference Wavelength 750 nm 750 nm
Plate Columns 1-4 5-12
™
For your convenience, we’ve included a worksheet at the end of this
chapter for recording the dispense weights, Delta OD values, calcula-
tions, and pass/fail. Make two copies of this worksheet—one for each
dispenser tested.
™
Tip : You can take advantage of KC4’s PowerReports feature to create
Microsoft Excel spreadsheets for performing the Results Analysis
calculations. See KC4’s User Guide for information on PowerReports.