Biotek Synergy HT, Руководство оператора

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Flushing/Purging the Fluid Path
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Synergy HT Operator's Manual
Flushing/Purging the Fluid Path
Applies only to Synergy™ HT models with injectors.
At the end of each day that the dispense module is in use, flush the fluid path using
Gen5’s or KC4’s priming utility. Leave the fluid to soak overnight or over a weekend, and
then
purge the fluid before using the instrument again.
Note:
This flushing and purging routine is also recommended before disconnecting the
outlet tubes from the rear of the reader, and before decontamination to remove any assay
residue prior to applying isopropyl alcohol or sodium hypochlorite.
To
flush the fluid path:
1. Fill two supply bottles with deionized or distilled water. Insert the supply
(inlet) tubes into the bottles.
2.
Place the priming plate on the carrier.
3. Gen5: Select System|Reader Control|Synergy(Com<#>) .
KC4: Select System|Reader Control .
4. Click the Dispenser tab and select Dispenser 1
.
5.
Set the
Volume
to 5000 L. Keep the default prime
Rate
.
6. Click Prime to start the process. When the process is complete, carefully
remove the priming plate from the carrier and empty it.
7. Repeat the process for Dispenser 2
.
Leave the water in the system overnight or until the instrument will be used
again. Purge the fluid from the system (see below) and then prime with the
dispense reagent before running an assay.
To purge the fluid from the system:
1. Place the inlet tubes in empty supply bottles or a beaker.
2. Gen5: Select System|Reader Control|Synergy(Com<#>) .
KC4: Select System|Reader Control .
3. Click the Dispenser tab and select Dispenser 1 .
4. Set the Volume to 2000 L.
5. Click Purge to start the process.
6. When the purge is complete, repeat the process for Dispenser 2 .
™
After purging the system, you may wish to run a quick Dispense
protocol to visually verify the dispense accuracy. See the next page
for instructions for creating the protocol.