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Chapter 3: Getting Started
BioTek Instruments, Inc.
To create an
Absorbance
protocol in KC4™:
1.
Select
Data|New Plate
. If prompted to select a protocol, select “Empty
Protocol” and click
OK
. If
not
prompted, select
Protocol|New
.
2.
Select
Protocol|Reading
. The Reading parameters dialog will appear.
3.
Set
Detection Method
to Absorbance.
4.
Select a
Reading Type
of Endpoint, Kinetic, or Spectrum. For a Kinetic
protocol, click the
Kinetic
button to define the Run Time and Interval.
5.
Select or specify the
Wavelengths
at which the plate will be read.
6.
Select a
Plate Type
.
7.
Enable
Temperature Control
and/or
Shaking
if necessary. Select any
Pre-Reading
parameters.
8.
Click
OK
to verify the parameters and return to the main screen.
9.
Select
Protocol|Save As
and give the protocol an identifying name.
To create a
Fluorescence
or
Luminescence
(non-Dispense) protocol in KC4:
1.
Select
Data|New Plate
. If prompted to select a protocol, select “Empty
Protocol” and click
OK
. If
not
prompted, select
Protocol|New
.
2.
Select
Protocol|Reading
. The Reading parameters dialog will appear.
3.
Set
Detection Method
to Fluorescence or Luminescence.
4.
Select a
Reading Type
of Endpoint or Kinetic. For a Kinetic protocol, click
the
Kinetic
button to define the Run Time and Interval.
5.
If supported by the current Synergy™ HT model, checking
Time-Resolved
enables the Time Resolved Fluorescence feature.
6.
Define the
Filter Sets
by selecting the filter(s), Optics Position, and
Sensitivity.
7.
Select a
Plate Type
.
8.
Enable
Temperature Control
and/or
Shaking
if necessary. Select any
Pre-Reading
parameters.
9.
Click
OK
to verify the parameters and return to the main screen.
10.
Select
Protocol|Save As
and give the protocol an identifying name.
Содержание Synergy HT
Страница 1: ...Operator s Manual Multi Mode Microplate Reader Synergy HT ...
Страница 2: ......
Страница 22: ...xx Preface BioTek Instruments ...
Страница 142: ...120 Chapter 4 Instrument Qualification BioTek Instruments Inc ...
Страница 144: ...122 Chapter 4 Instrument Qualification BioTek Instruments Inc ...
Страница 174: ...152 Chapter 5 Preventive Maintenance BioTek Instruments Inc ...
Страница 186: ...164 Appendix B Computer Control BioTek Instruments Inc ...
Страница 236: ...214 Appendix D Specifications BioTek Instruments Inc ...
Страница 240: ...218 Appendix E Instrument Dimensions for Robotic Interface BioTek Instruments Inc ...
