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Chapter 4: Instrument Qualification
BioTek Instruments, Inc.
Test Procedure: KC4™
Note: If you are using one of BioTek’s keypad-based readers, such as
the ELx800™ or ELx808™, make sure the reader is
not
running in
Rapid mode. To check the setting, select UTIL
Æ
READ and cycle
through the options until READ IN RAPID MODE? appears. Set it to NO.
Perform the following set of steps
two times
, to test both dispensers.
1.
Using KC4, prime the fluid lines with deionized water:
•
Fill a reagent bottle with deionized water and insert the inlet tube.
•
Select
System|Reader Control
and click the
Dispenser
tab. Set the
Dispenser number (1 or 2), enter a
Volume
of 4000 µL, and click
Prime
.
Place the priming plate on the carrier and click
OK
.
•
Remove the inlet tube from the bottle and run another prime with the
volume set to 2000 µL. Empty the plate and replace it on the carrier.
2.
Fill a reagent bottle or beaker with 20 mL of the green dye solution. Prime the
fluid lines with 2000 l of the green dye. Remove and empty the priming plate.
Perform these steps carefully. When each dispense protocol is finished,
you will weigh the plate, record the weight, and tare the balance.
3.
Place a new 96-well microplate on the balance and tare the balance.
4.
Place the plate on the carrier and run the
80
μ
l Dispense
protocol created for
the dispenser under test.
5.
Weigh the plate. Record the 80 µL dispense weight, and then tare the balance.
6.
Place the plate on the carrier and run the
20
μ
l Dispense
protocol created for
the dispenser under test.
7.
Weigh the plate. Record the 20 µL dispense weight, and then tare the balance.
8.
Place the plate on the carrier and run the
5
μ
l Dispense
protocol created for
the dispenser under test.
9.
Weigh the plate. Record the 5 µL dispense weight.
10.
Pipette or dispense
150 µL
of deionized water into each well, on top of the
green dye solution.
11.
If the reader does not support shaking, shake the plate in an orbital shaker for
about 15 seconds.
12.
Transfer the plate to the absorbance reader and run the two Read protocols as
described under
Test Setup—KC4
on page 105. Save each Data file using an
identifying file name (
Data|Save As
).
13.
When all tests are complete, prime both dispensers with at least 5000 µL of
deionized water to flush out the green dye solution.
Содержание Synergy HT
Страница 1: ...Operator s Manual Multi Mode Microplate Reader Synergy HT ...
Страница 2: ......
Страница 22: ...xx Preface BioTek Instruments ...
Страница 142: ...120 Chapter 4 Instrument Qualification BioTek Instruments Inc ...
Страница 144: ...122 Chapter 4 Instrument Qualification BioTek Instruments Inc ...
Страница 174: ...152 Chapter 5 Preventive Maintenance BioTek Instruments Inc ...
Страница 186: ...164 Appendix B Computer Control BioTek Instruments Inc ...
Страница 236: ...214 Appendix D Specifications BioTek Instruments Inc ...
Страница 240: ...218 Appendix E Instrument Dimensions for Robotic Interface BioTek Instruments Inc ...
