Dispense Module Tests
|
109
Synergy HT Operator's Manual
Results Analysis
For your convenience, we’ve included a
worksheet
at the end of this
chapter for recording the dispense weights, Delta OD values, calcula-
tions, and pass/fail. Make two copies of this worksheet—one for each
dispenser tested.
The pass/fail criteria for each set of 32 wells with the same dispense volume is
based on the calculated coefficient of variation (%CV) and Accuracy % Error.
•
If you created the Gen5™ protocols as described on pages 110–113, the
%CVs are calculated automatically. With the experiment open, click the
Statistics
button and set the Well Type to
Assay Control
. Use the Data
drop-down box to select each Delta OD data set.
•
If you created the KC4™
Read
protocols as described on page 117, the %CV
is calculated automatically. With the Data file open, select the
Delta OD
data set and click the
Statistics
button.
For each volume dispensed (80, 20, 5 µL), for each dispenser (1, 2):
•
Calculate the
Standard Deviation
of the 32 wells
•
Calculate the
Mean
of the 32 wells
•
Calculate the
%CV
:
(Standard Deviation / Mean) x 100
•
Calculate the
Accuracy % Error
:
((Actual Weight – Expected Weight) / Expected Weight) * 100
Expected Weights
for 32 wells: 80
μ
l (2.560 g), 20
μ
l (0.640 g),
5
μ
l (0.160 g). It is assumed that one gram is equal to one milliliter.
Dispense
Volume
To pass,
% CV must be:
To pass, Accuracy
% Error must be:
80
μ
l
≤
2.0%
≤
2.0%
20
μ
l
≤
7.0%
≤
5.0%
5
μ
l
≤
10.0%
≤
20.0%
Failures
If any tests fail, prime the fluid lines and rerun the test(s). If the test(s) fail
again, the injector heads may require cleaning (see
Chapter 5, Preventive
Maintenance
).
If tests continue to fail, contact BioTek’s Technical Assistance Center.
Содержание Synergy HT
Страница 1: ...Operator s Manual Multi Mode Microplate Reader Synergy HT ...
Страница 2: ......
Страница 22: ...xx Preface BioTek Instruments ...
Страница 142: ...120 Chapter 4 Instrument Qualification BioTek Instruments Inc ...
Страница 144: ...122 Chapter 4 Instrument Qualification BioTek Instruments Inc ...
Страница 174: ...152 Chapter 5 Preventive Maintenance BioTek Instruments Inc ...
Страница 186: ...164 Appendix B Computer Control BioTek Instruments Inc ...
Страница 236: ...214 Appendix D Specifications BioTek Instruments Inc ...
Страница 240: ...218 Appendix E Instrument Dimensions for Robotic Interface BioTek Instruments Inc ...