Biotek Synergy HT, Руководство оператора

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Chapter 3: Getting Started
BioTek Instruments, Inc.
Controlling the Dispense Module
This section applies to Synergy™ HT models with injectors only.
Gen5™ is used to perform several dispense module-specific functions, including
initializing, priming, and purging. Gen5 also contains certain configuration items that
must be set before using the dispense module. Read the following sections to become
familiar with these functions and configuration items.
Initialization
If the dispense module was connected to the reader before the reader was turned
on, or if a System Test was run via Gen5, the dispense module should initialize
automatically. If for any reason the module does not initialize automatically, you
can initialize it from Gen5:
1. In Gen5, select System|Reader Control|Synergy(Com<#>) and click
the Dispenser tab.
2. Select the desired Dispenser number (1 or 2) and click the Initialize
button. The syringe drive will move to its home position and its sensors will
be verified. Upon successful completion, the
Initialized field should show
“Yes”.
Prime Utility
Before running an experiment with a Dispense step, the dispense module and its
associated tubing must be primed with the fluid to be used. Gen5 provides a
special utility for this task. To prime the dispense module:
1. Fill the supply bottle with a sufficient volume of the fluid to be used for the
prime and the assay. Insert the appropriate inlet tube into the bottle.
2. Important! Place the priming plate on the carrier.
3. In Gen5, select System|Reader Control|Synergy(Com<#>) and click
the Dispenser tab.
4. Select the Dispenser number (1 or 2) associated with the supply bottle.
5. Enter the Volume to be used for the prime, from 5 to 5000 L.
The minimum recommended prime volume is 1100 L.
6. Select a prime Rate , in L/second.
7. Click Prime to start the process.
8. When the process is complete, carefully remove the priming plate from the
carrier and empty its contents. If the priming plate is empty, the prime
volume was too low.