YOKOGAWA CQ1, User Manual, Page: 86 / 268

4-38
<4. Measurement Procedures>
IM 80J01A01-01E
<About CQ1 autofocus>
CQ1 can perform 2 types of autofocus, which are laser based and
image based.
Laser Autofocus detects surface of bottom plate or cover glass by laser
and set the surface as Z base position. Please note that surface to
detect (Z base positon) is different by the kind of sample vessel. (Refer
to following table.)
Image Autofocus acquires image by designated channel and sets the Z
position that maximum intensity is obtained as Z base position.
Microplate
①
Laser Autofocus detecting surface
(slide glass)
②
Laser Autofocus detecting surface
(microplate, dish, cover glass chamber)
※
③
Image Autofocus detecting position
Dish
Slide
Cover glass chamber
※
In case that laser autofocus
doesn’t operete normally when using microplate, dish
or cover glass chamber, surface to detect would be changed to lower surface of
bottom plate of sample vessel. For details, please refer to page
5-20 .
In
“Map” window, select “Rec Mode” (refer to page
5-96 ) and select
measuring Area by mouse drug.
Culture medium and others
Bottom plate,
Cover glass
① ② ③
Select “Rec mode”
Drag the Area for
measuring.