YOKOGAWA CQ1, User Manual, Page: 22 / 268

1-2
<1. Function Overview>
IM 80J01A01-01E
Measurement values
CQ1 outputs the following measurement values by analyzing confocal
images of fluorescently labelled each cell.
Measurement Unit Measurement
Method Description
Morphological
features Area µm
2
2D
measurement Measure the area of objects.
Circumference µm 2D
measurement Measure the circumference of object.
Circularity - 2D
measurement Compare the area of the circumscribed
circle and the area of the object.
Circularity is expressed by a positive
number of less than 1.0, and perfect circle
is 1.0
Volume µm
3
3D
measurement Measure total volume of objects.
Surface area µm
2
3D
measurement Measure total surface area of objects.
Sphericity - 3D
measurement Compare the volume of an intracellular
structure and its circumscribed globe.
Sphericity is expressed by a positive
number of less than 1.0, and perfect globe
is 1.0.
Diameter µm 2D
measurement Measure diameter of the circumscribed
circle of an object.
3D
measurement Measure diameter of the circumscribed
globe of an object.
Intensity Sum - 2D/3D
measurement Measure the sum of pixel intensity of
objects.
Average - 2D/3D
measurement Measure average pixel intensity of
objects.
Standard
deviation - 2D/3D
measurement Measure standard deviation of pixel
intensity of objects.
Minimum - 2D/3D
measurement Measure minimum pixel intensity among
objects.
Maximum - 2D/3D
measurement Measure maximum pixel intensity among
objects.