YOKOGAWA CQ1 User Manual Download Page 127

Page: 127 / 268

<5. Functions of CQ1 Software>

5-31

IM 80J01A01-01E

(5) Objective Lens

Select objective lens here. Selected lens is highlighted.

(6) Time-lapse Setting

Switch the time-lapse acquisition mode (normal mode/ fast mode

(option)).

If you

didn’t buy this option, this menu isn’t shown and the time-lapse

acquisition mode is always normal mode.

①

Normal

In this mode, sequence of imaging in each well, field, channel and

Z slice has a priority. These sequences of imaging are performed

at interval and duration set at

“Time-lapse Condition” described

below.

②

Fast (option)

In this mode, time-lapse imaging has a priority. Time-lapse

imaging of speed and time length set at

“Fast Time-lapse

Condition

” is performed in each well and field. Numbers of channel

and Z slice are fixed to

“1”. When switching to this mode, the

selected channel remains and the others are removed

automatically. Also,

“Chromatic Abberation” becomes invalid

automatically.

This mode is not correspond to on-the-fly analysis. To analyze

image data acquired by this mode, it is necessary to acquire image

data by CQ mode then analyze by HDD mode.

①

②

Note

Don

’t select “Fast” mode if setting of folder structure of saving

data is

“By Time Point” (Refer to

5.11 User Setting

In case to use

“Fast” mode, select Main Menu -> “Option” ->

“User Setting” and select setting other than “By Time Point”.

Summary of Contents for CQ1

Page 1: ...s Manual Confocal Quantitative Image Cytometer IM 80J01A01 01E The translation of the original instructions However warning labels are not the same IM 80J01A01 01E Yokogawa Electric Corporation 10th Edition ...

Page 2: ...This document corresponds to the following versions of software Measurement software R1 04 ...

Page 3: ...red with the utmost care however if you have any questions or note any errors please contact our branch or sales office of the neighborhood Unauthorized reproduction or reprinting of this manual in whole or in part is prohibited Follow the operating instructions to keep stable system operation Trademarks Microsoft and Windows are registered trademarks or trademarks of Microsoft Corporation in the ...

Page 4: ...rantee regarding its safety and function Following symbols are used in the manual in the following cases WARNING This symbol describes precautions to avoid hazardous situations that could result in a bodily injury or death of the user upon mishandling and or incorrect use of the equipment CAUTION This symbol describes precautions to avoid hazardous situations that could result in a minor bodily in...

Page 5: ...s in emission of visible and invisible Class 3B laser beam and that entry of this beam into the eyes or its contact with the skin is a potential health hazard Warning label 4 This label warns the users on dangers that require safety control measures when using the laser To be specific the users are cautioned that opening this cover results in emission of visible and invisible Class 3B laser beam a...

Page 6: ...iv IM 80J01A01 01E Location of Warning Labels Right Side of Main Unit Rear Panel of Main Unit Top Panel of Main Unit Warning label 3 Explanation label 1 Warning label 4 Warning label 3 Warning label 3 ...

Page 7: ... 01E Rear Panel of Main Unit Transmission Illumination Option Front Panel of Utility Box Rear Panel of Utility Box Warning label 3 Warning label 3 Warning label 1 Warning label 1 Warning label 2 Explanation label 2 ...

Page 8: ...vi IM 80J01A01 01E Right Side of Utility Box Aperture label ...

Page 9: ...xer CQ1 emits max 20 CO2 mixture gas at a rate of 200ml min If CO2 concentration in the air exceeds 3 it may be harmful to the human body Be sure to provide ventilation lead exhaust gas from CQ1 to outside of the room or take other appropriate measures It is also recommended that an alarm system be installed in case of emergency If you use Gas Mixer CQ1 emits min 0 1 low O2 gas at a rate of 200ml ...

Page 10: ...anges in the temperature in places subject to dew condensation or corrosive or combustible gases Never touch any of the internal parts of this product And never take apart or modify this product The optical system housed in the product may become dirty damaged or out of calibration and so on leading to equipment failure If the equipment has malfunctions or damages please immediately cease use and ...

Page 11: ...ws Class 1 Essentially safe Continue to look into the Class 1 laser will not cause any danger Class 2 If the Class 2 laser is looked into for a period not more than 0 25 second there is no risk of danger Class 3B Looking into the laser beam from this class of equipment is dangerous Even if the specification of the laser equipment used does not exceed this class there is still a risk of eye injury ...

Page 12: ...ssified as a Monitoring and Control instrument product When disposing this instrument in the EU contact the distributer whom you bought it from Do not dispose in domestic household waste Indemnity Yokogawa shall provide no warranty regarding this product unless otherwise specified separately in the Warranty Rule Yokogawa shall assume no responsibility for any loss suffered by a customer or third p...

Page 13: ...hased your product CQ1 Part Quantity Description CQ1 Main Unit 1 Confocal fluorescent microscope system to observe cells cultured in vessels such as Microplate or slide glass USB cable 1 Communication cable to connect CQ1 main unit and workstation Main Unit UTB cable 1 Power supply and communication cable to connect CQ1 main unit and utility box Utility Box 1 Equipment for supply power and excitat...

Page 14: ... connector on Utility Box Objective Lens Part Quantity Description Objective lens 1 6 To be installed on CQ1 main unit One to six lenses are installable depending on the specification Please refer to Parts List CMPL 80J01A01 01 for details Workstation Part Quantity Description Workstation 1 Attached with mouse key board back up CD and instruction manual AC Cord WS 1 AC cord for workstation Supplie...

Page 15: ... field observation There are following 2 types Bright field Phase contrast switchable type manual Phase contrast type Gas mixer TOKAI HIT GM 8000 1 Supply mixture gas that O2 and CO2 concentration is contolled to stage attachment Gas mixer connecting tube 1 Tube to connect CQ1 main unit and Gas mixer Attached if stage heater option is selected Stage attachment All wells imaging type 1 Stage attach...

Page 16: ... slide glass ISO 8037 1 1 Sample holder for set four slide glasses ISO 8037 1 Sample holder for cover glass chamber 1 Sample holder for set one cover glass chamber 60 mm dish ring 1 Hold silicone rubber when using chambered type stage attachment and 60 mm dish Rubber for 60 mm dish 1 Set on 60 mm dish when using chambered type stage attachment Holding plate for CGC 1 Hold silicone rubber when usin...

Page 17: ...xv IM 80J01A01 01E Sample holders are set into the stage attachment as shown below Sample Sample holder Stage attachment XY stage ...

Page 18: ... Down the Equipment The name of each part and starting shutdown of the equipment are explained 4 Measurement procedures Procedures for measurement are explained 5 Functions of measurement software Screen of measurement software is explained 6 Maintenance Maintenance parts and consumable parts are explained 7 Troubleshooting Error messages and troubleshooting procedures for this equipment are expla...

Page 19: ...ower 2 7 3 Name of Each Part Starting and Shutting Down the Equipment 3 1 3 1 Name and Function of Each Part 3 1 3 2 Starting and Shutting Down of CQ1 3 8 3 3 Starting and Shutting Down of Gas Mixer Option 3 13 4 Measurement Procedures 4 1 4 1 Measurement Procedures 4 1 4 2 Basic Protocol Setting 4 31 4 3 Sample Searching 4 33 4 4 Imaging Setting 4 36 4 5 Analysis Protocol Setting 4 39 4 6 Object ...

Page 20: ...126 5 11 User Setting 5 127 5 12 Activation Setting 5 128 5 13 Advanced Editor 5 129 6 Maintenance 6 1 6 1 Maintenance of Workstation 6 1 7 Trouble Shooting 7 1 7 1 Trouble Shooting during Measurement Preperation 7 1 7 2 Trouble Shooting during Imaging 7 4 7 3 Trouble Shooting during Analyzing 7 15 7 4 Other Trouble Shootings 7 17 8 Specifications 8 1 8 1 Details of MS codes 8 1 8 2 General Specif...

Page 21: ...n without breaking cell mass or detaching cell layers from culture dish In addition to two dimensional information such as the area various three dimensional information such as volume surface area cell number and location granule location within each cell fluorescent intensity can be well visualized and displayed as graphs Live cell observation Our proprietary confocal scanner unit CSU series is ...

Page 22: ...cts Surface area µm 2 3D measurement Measure total surface area of objects Sphericity 3D measurement Compare the volume of an intracellular structure and its circumscribed globe Sphericity is expressed by a positive number of less than 1 0 and perfect globe is 1 0 Diameter µm 2D measurement Measure diameter of the circumscribed circle of an object 3D measurement Measure diameter of the circumscrib...

Page 23: ...multiple scans over the observation area with approximately 1 000 laser beams Multiple beam scans are performed not only at high speed but also with each beam exciting fluorochromes at high efficiency and very low laser intensity These results in an optimal live cell observation system where phototoxicity and fluorescence photobleaching are suppressed notably compared to any conventional systems w...

Page 24: ......

Page 25: ... not work normally by these changes Do not install the third party software which YOKOGAWA doesn t specify to Workstation The performance of CQ1 may reduce In addition 8 3 filename generation of Windows NTFS file system is invalid in Workstation for performance improvement Therefore the third party software may not work normally Do not install any third party hardware to CQ1 Main Unit Utility Box ...

Page 26: ...ns specified below Please call service personnel when you install or move the equipment Installation example 1 In case of using CQ1 normal gas mixer Note Please prepare following gas cylinder and regulator Gas cylinder Gas N2 100 CO2 100 Regulator Outlet pressure Settable in 0 1 0 15MPa Piping Silicone tube inside diameter 6 mm outside diameter 10 mm is connectable ...

Page 27: ... 2 Precautions for Use Installation 2 3 IM 80J01A01 01E Installation example 2 In case of using gas mixer that user prepares ...

Page 28: ...700 mm x D 500 mm x H 700 mm or larger Withstand load 100 kg or larger Utility Box Minimum Clearance Back 50 mm Above 50 mm Side 50 mm Avoid placing on floor directly Gas Mixer Minimum Clearance Back Provide enough space not to buckle tubes and cables Side 50 mm Avoid placing on floor directly Avoid installing at highly humid and or hot place such as close to heaters or under direct sunshine or fr...

Page 29: ...eam dust corrosive gases and so on Location where electric power supply system of more than AC100V 14A 1 400W is available 1 Subjecting the equipment to a sudden temperature shift may cause condensation Installation Environment Operating Temperature Range CQ1 15 35 Gas Mixer 20 35 Operating Humidity Range CQ1 20 70 RH non condensing Gas Mixer 10 85 RH non condensing ...

Page 30: ...on the equipment is turned off CAUTION Please don t connect any cables other than specified by YOKOGAWA Workstation Utility Box CQ1Main Unit Fiber AC Code CQ1 Power Supply Camera Link Cable USB Cable Gas Mixer CO2 N2 Joint Display Cable AC Code Display AC Code WS Main Unit Utility Box Cable Remote Interlock AC Code Gas Mixer Gas Mixer Tube ...

Page 31: ...instrument In case any problems happened as a result of such inappropriate processing you are liable for the cost of repair Install patches Windows Update service pack security patch to update operating system Install any third party software which YOKOGAWA doesn t specify Install any third party hardware Use any third party software and hardware Connect to the internet 2 4 Connecting the Power Co...

Page 32: ...ble supplied with this equipment is a 3 pin type with a grounding wire Accordingly use a 3 pin power socket with a protective grounding terminal If a 3 pin to 2 pin conversion adapter usable only in Japan is used securely connect the grounding wire of the conversion adapter to the protective grounding terminal Need for protective grounding Do not cut the protective grounding wire running inside or...

Page 33: ... be removed except by Yokogawa s service personnel Some parts in this equipment carry high voltage and a Class 3B laser is also housed inside Accordingly removing the case presents dangerous situations External connections Connect each external device after providing proper protective grounding ...

Page 34: ......

Page 35: ...pen door and load sample vessels such as microplate dish or slide glass here 2 POWER Lamp Lights when the power switch on the Utility Box is ON and lights out when the power switch is OFF 3 READY Lamp Lights when the initialization of this equipment finished and get ready to use 4 Door OPEN CLOSE Button OPEN CLOSE the door of the sample loading port 5 LASER Lamp Lights when the laser emits and lig...

Page 36: ...mination unit is set on this part 9 Fiber Port Port to connect the fiber to CQ1 Main Unit 10 USB Connector Connector for USB cable connection with the Workstation cable 11 Gas Inlet Port Port to connect the tube from Gas Mixer option 12 Camera Cable Port Inlet port to insert camera link cable to CQ1 13 Power Cable Connector Port to connect the power cable from Utility Box 14 Gas Outlet Port Port t...

Page 37: ...the Equipment 3 3 IM 80J01A01 01E Transmission Illumination 1 Switching lever bright field phase contrast Switch the method of transmission illumination Pull the lever toward to switch to bright field Push the lever back to switch to phase contrast 1 ...

Page 38: ...lights out when the switch is off 3 MAIN UNIT Lamp Lights when connected with CQ1 Main Unit 4 GAS MIXER Lamp Not in use 5 READY Lamp Lights when lasers are enable and lights out when lasers are disable and flicks when lasers are initializing 6 EMISSION Lamp Lights when laser emits and lights out when laser is off 7 Power Switch Switch ON to power on CQ1 Main Unit Switch OFF to power off CQ1 Main U...

Page 39: ...able Connector Port to connect power cable 10 Maintenance Connector Not in use 11 Remote Interlock Connector Port to connect lase interlock connector 12 Reserved Connector Not in use 13 CQ1 Main Unit Power Cable Connector Port to connect power cable 14 Fiber Port Port to insert the fiber to Utility Box 9 8 10 11 12 13 14 ...

Page 40: ... gas 3 O2 Concentration Controller to set O2 Concentration of output mixture gas 4 Gas Flow Controller to set flow rate of output mixture gas 5 100 CO2 IN Port to connect the tube from CO2 gas cylinder via the regulator 6 100 N2 IN Port to connect the tube from N2 gas cylinder via the regulator 7 Mix Gas OUT Port to connect the tube to CQ1 Main Unit 8 GM LINK Not in use 9 DC IN Port to connect cab...

Page 41: ... the Equipment 3 7 IM 80J01A01 01E Workstation 1 USB Connector Connect to the USB connector 8 on the CQ1 Main Unit Connect supplied cable as shown above 2 Camera Link Cable Connector Connect camera link cable out of CQ1 Main Unit as shown above 1 2 ...

Page 42: ...cted Refer to 2 3 Wiring the Peripherals Immediately pull out the AC cord from inlet of equipment when you notice smoke coming out of the main unit foul smell abnormal noise or any other abnormality 2 Power ON the Workstation 3 Confirm that there is enough free space in data D disk drive Data volume of one image that CQ1 outputs is about 11MB Keep free space in data D disk drive by considering tot...

Page 43: ...utting Down the Equipment 3 9 IM 80J01A01 01E 4 Wait until Ready lamp on Utility Box turn ON after blinking It takes about 1 minute from turning the key switch on Click the desktop icon of the Workstation to start CQ1 Software READY lamp ...

Page 44: ...01E 3 2 2 Shutting Down CQ1 1 Select File menu Exit menu or click the close box on the main screen of CQ1 Software window 2 Click OK button In case that measurement is running following message is shown Confirm that there are no problem to quit measurement then click OK close box ...

Page 45: ...art Starting and Shutting Down the Equipment 3 11 IM 80J01A01 01E 3 Shut down Workstation 4 Turn the key switch on the Utility Box to OFF 5 Turn the key switch OFF then turn the power switch OFF Power Switch Key Switch ...

Page 46: ...01 01E 3 2 3 Offline Mode You can start CQ1 Software by offline mode without turning equipment on It is convenient for viewing data or analyzing without measurement Starting procedure is different from above Select CQ1 Offline from Windows start menu It is not icon on desktop ...

Page 47: ...erclockwise rotating 3 Open valve of gas cylinder 4 Open valve of regulator 5 By clockwise rotating valve adjust outlet pressure meter B to be in the range of 0 1 MPa 0 15 MPa 6 Turn the power switch of Gas Mixer 3 3 2 Shutting Down Gas Mixer 1 Close valve of regulator to stop gas supply If the gas cylinder will not be used for long time close valve of gas cylinder after closing valve 2 Turn the p...

Page 48: ......

Page 49: ...Procedures of Stage Attachment If you don t need to exchange stage attachment go to procedure shown in 4 1 5 Setting Samples Features of each stage attachment are as following All Well Imaging Type Chambered Type Sealing Blocks Capable of imaging all wells of microplate Capable of keeping the temperature CO2 O2 concentration and humidity Note The range which can be imaged is different between type...

Page 50: ...maging Type to Chambered Type Click Sample tab OPEN Button of CQ1 Software or do long press 3 seconds of Door OPEN CLOSE Button of CQ1 Main Unit to open the door Turn the key switch OFF then turn the power switch OFF Loosen 2 screws Door OPEN CLOSE Button Power Switch Key Switch ...

Page 51: ...rement Procedures 4 3 IM 80J01A01 01E Push lever and lift up to remove Stage Attachment CAUTION Lift up Stage Attachment vertically because it is positioned by pin Be careful not to collide with cover Lever Pin ...

Page 52: ...dures IM 80J01A01 01E Prepare Stage Attachment to set Direct clamp as following diagram and set Stage Attachment Be careful not to collide with cover Put Stage Attachment on the stage and set it with pushing lever Clamp Lever ...

Page 53: ...ION Be careful not to touch sample setting part of Chambered Type Stage Attachment when put it on the stage This part is easily broken Fasten 2 screws CAUTION Confirm that Stage Attachment does not tilt Sample setting part Stage Attachment Stage OK NG ...

Page 54: ...4 6 4 Measurement Procedures IM 80J01A01 01E Set Sealing Block for Clamp on the clamp In case of using microplate set Sealing Block for Microplate as following diagram ...

Page 55: ... switch ON CAUTION After turning power switch ON the door closes automatically Please be careful not to pinch your fingers or others Immediately pull out the AC cord from inlet of equipment when you notice smoke coming out of the main unit foul smell abnormal noise or any other abnormality ...

Page 56: ...Imaging Type Click Sample tab OPEN Button of CQ1 Software or do long press 3 seconds of Door OPEN CLOSE Button of CQ1 Main Unit to open the door Turn the key switch OFF then turn the power switch OFF Remove Sealing Block for Microplate if it is set Door OPEN CLOSE Button Power Switch Key Switch ...

Page 57: ...ove Sealing Block for Clamp Exchange Stage Attachment referring to page 4 1 CAUTION Be careful not to touch sample setting part of Chambered Type Stage Attachment when remove it from the stage This part is easily broken Sample setting part OK NG OK NG ...

Page 58: ... switch ON CAUTION After turning power switch ON the door closes automatically Please be careful not to pinch your fingers or others Immediately pull out the AC cord from inlet of equipment when you notice smoke coming out of the main unit foul smell abnormal noise or any other abnormality ...

Page 59: ...you don t need environment control go to procedure shown in 4 1 5 Setting Samples In case that measurement is running click Pause button to pause Click Sample tab OPEN Button of CQ1 Software or do long press 3 seconds of Door OPEN CLOSE Button of CQ1 Main Unit to open the door Turn the key switch OFF then turn the power switch OFF Door OPEN CLOSE Button Power Switch Key Switch ...

Page 60: ...r CAUTION Please don t remove screws from bath cover During temperature controlling water drops are formed on the downside of the bath cover Please be careful not to drop water inside the equipment when removing bath cover If water drops inside the equipment wipe it completely before turning CQ1 on Bath cover Water drop ...

Page 61: ... 4 Measurement Procedures 4 13 IM 80J01A01 01E If the waterbath is empty pour 25 ml of distilled water It is recommended to pour by syringe Pour distilled water to cover whole area of the bath OK NG ...

Page 62: ...poured it causes water immersion into the equipment and failure of the equipment CAUTION After measurement wipe water in the bath and on the downside of bath cover everytime NG Note Appropriate humidity level is maintained over 6 hours when 25 ml of water is poured Water drop on the downside of bath cover Water remains in the bath ...

Page 63: ...roperly Set sub cover and tighten 2 screws Turn the power switch ON then turn the key switch ON CAUTION After turning power switch ON the door closes automatically Please be careful not to pinch your fingers or others Immediately pull out the AC cord from inlet of equipment when you notice smoke coming out of the main unit foul smell abnormal noise or any other abnormality Power Switch Key Switch ...

Page 64: ...n as following sequence Interval Interval Interval Interval Interval Interval Interval Interval Interval Interval Interval Interval Measuring Timepoint Interrupting No interrupting Interrupting time is in interval Interrupting time is over interval In case of the time lapse measurement that pause for filling water and others is supporsed it is recommended to set interval so that time span from fin...

Page 65: ... Mixer Option In this section procedures are described for example by setting as following Sample vessel microplate CO2 concentration in chamber 5 O2 concentration in chamber atomostic concentration 20 For other settings please refer to Technical Information TI 80J01A05 01E Setting Parameter of Gas Mixer By Up Down key of GAS FLOW set mixture gas flow rate to 150 ml min and push Enter By Up Down k...

Page 66: ... of Setting Stage Heater This procedure is only perfomed when controlling environment around sample If you don t perform environment control go to procedure shown in 4 1 5 Setting Samples Click ON button in Environment window Temperature Control Click Edit button ...

Page 67: ... of chamber temperature depends on room temperature refer to following chart For example to set chamber temperature 37 set room temperature 20 30 10 15 15 20 20 25 30 35 25 30 35 40 45 Room Temperature Chamber Temperature Note If 35 mm dishes is used input control temperature 0 8 example If you want to set chamber temperature to 37 input 36 2 to Setting ...

Page 68: ...perature of sample vessel changes rapidly after it is set into CQ1 strain of sample vessel occurs and autofocus may not operate normally In case of unusing Stage Heater set sample into CQ1 after acclimating to room temperature In case of the sample taken from refrigerator acclimating time is about 30 minutes In case that condensation occurs on the bottom of sample vessel please wipe it away In cas...

Page 69: ...t dishes on sample holder Lift up leaf spring set a dish and push down leaf spring to fix a dish CAUTION Same dishes must be set on all 3 positions Sample holder for 35 mm dishes Lift up leaf spring Set a dish and push down leaf spring to fix a dish Set dishes on all 3 positions ...

Page 70: ...Measurement Procedures IM 80J01A01 01E In case of using 60 mm dish Only when controlling environment Set rubber on the dish Set dish on sample holder Only when controlling environment Set a dish ring Dish ring ...

Page 71: ...Procedures 4 23 IM 80J01A01 01E Fix a dish by leaf spring Lift up leaf spring Set a dish and push down leaf spring to fix a dish In case of performing environment control In case of not performing environment control ...

Page 72: ...ironment contol Set slides on sample holder Set a slide on sample holder with pressing to leaf spring Please be careful not to tilt slides Sample holder for slide glass Set a slide with pressing to leaf spring Please set slides such as label comes near side basically depend on the slide glass setting in CQ1 Software refer to page 5 19 ...

Page 73: ... 4 Measurement Procedures 4 25 IM 80J01A01 01E CAUTION Set slides not to put cover glass on sample holder Cover glass Slide glass Sample holder ...

Page 74: ...olling environment Remove rid from cover glass chamber set rubber on the cover glass chamber fastly and set rid again Set cover glass chamber pushing A1 well to corner indicated by arrow For IWAKI 2 holes For NUNC Lab Tec I 3 holes For NUNC Lab Tec II 4 holes For MATSUNAMI 5 holes Rubber list OK NG ...

Page 75: ...y when controlling environment Set a holding plate Fix a cover glass chamber by leaf spring Holding plate In case of performing environment control In case of not performing environment control Set a cover glass chamber and push down leaf spring to fix ...

Page 76: ...n Unit set microplate or sample holder on stage Click Close Button of CQ1 Software or do long press 3 seconds of Door OPEN CLOSE Button of CQ1 Main Unit to close the door CAUTION Be careful not to stuck fingers or others in door If fingers or others are stuck CQ1 will stop In the case please turn Power Switch of Utility Box off then turn it on again Door OPEN CLOSE Button ...

Page 77: ...y it may result to equipment damage Please confirm that 4 corners of the microplate or sample holder are set on stage and it is moved lightly not to be stuck to edge of sample setting place CAUTION Immediately pull out the AC cord from inlet of equipment when you notice smoke coming out of the main unit foul smell abnormal noise or any other abnormality Projection Portion Projection Portion OK NG ...

Page 78: ...4 30 4 Measurement Procedures IM 80J01A01 01E If you perform measurement by protocol which has been made go to procedure shown in 4 8 Sample Measurement ...

Page 79: ...ata in Protocol window Select sample vessel in Sample window Designate parent folder Input suffix of folder name Input description if needed Note Please designate local drive D as data storage folder In case of designating other than local drive D performance of HDD decreases and it may cause errors during image acquisition ...

Page 80: ...ures IM 80J01A01 01E Switch to Imaging window Double click imaging channel setting part to open Channel Setting Set the lasers and emission filters for each imaging channel For details refer to 5 9 Channel Setting Double click ...

Page 81: ...ples exist suitable position to imaging by low magnification objective lens Set the viewer layout to Image Map In Imaging window select imaging channel well and objective lens For sample searching use low magnification objective lens 2x 10x Select imaging channel Select well Select objective lens ...

Page 82: ...hich samples exist After Z position search click to apply the result of searching to Z Stack parameter In Map window select Map Mode refer to page 5 95 and select sample searching region by mouse drug Click Start to start map imaging Apply the result of searching to Z Stack parameter Select Map mode Drag the region for searching ...

Page 83: ...to that for measurement and select suitable field for measurement Note Blue region in Map window can t be designated as imaging region because objective lens interferes with stage attachment sample holder and sample vessel Select Test imaging mode Select suitable field for measurement Change objective lens for that for measurement ...

Page 84: ...2 change Excitation Power and or Exposure of each imaging channel In case of using Transmission Illumination with Bright Field Phase Contrast Push the lever back in case of acquiring phase contrast image Pull the lever toward in case of acquiring bright field image Select imaging channel Change Excitation Power and or Exposure Push Back Phase contrast PH Pull toward Bright field BF ...

Page 85: ...Range and Slices As well set 1 to Slices and 2D measurement is executed Set 2 or more and 3D measurement is executed 3 Time lapse setting In Time lapse Condition window of Imaging window refer to page 5 32 change Interval and Duration If time lapse measurement is not performed set 1 to Duration Change Range and Slices Change autofocus setting Change Interval and Duration ...

Page 86: ...maximum intensity is obtained as Z base position Microplate Laser Autofocus detecting surface slide glass Laser Autofocus detecting surface microplate dish cover glass chamber Image Autofocus detecting position Dish Slide Cover glass chamber In case that laser autofocus doesn t operete normally when using microplate dish or cover glass chamber surface to detect would be changed to lower surface of...

Page 87: ... images for analysis setting In Wells window of Imaging window refer to page 5 25 make button to status green select a few wells from wells for measure selected wells become light blue and click PreRec Click Stop button to stop measurement Selection of negative control well s and positive control well s is effectual Select a few wells ...

Page 88: ...d previously Select Application tab if you use analysis protocol which is preloaded in CQ1 Software Cell Cycle FISH and so on Select Template tab if you use basic protocol which is preloaded in CQ1 Software Select analysis protocol and click Load to load analysis protocol For details refer to page 5 37 Check Select either User Application or Template Select analysis protocol ...

Page 89: ...eeded in Object Detect Setting window of Application window refer to page 5 44 change setting of object recognizing of each imaging channel If needed in Link Setting window of Application window refer to page 5 46 change link setting between each object ...

Page 90: ...s to confirm result of object recognizing If it is necessary to improve the result adjust recognizing condition as following If it is necessary to improve more click Edit open Advanced Editor and set detailed recognizing condition For details refer to 5 4 5 Analysis Window Open Advanced Editor Adjust recognizing condition Detect all objects Detect single object ...

Page 91: ... PreAnalyze Confirm that objects are detected properly in multiple wells If it is necessary to improve the result adjust detecting condition in the field which object isn t detected properly 4 7 Protocol Saving In Wells window of Imaging window refer to page 5 25 make button to status green select all wells for measurement in the protocol selected wells become light blue Select all wells for measu...

Page 92: ...4 44 4 Measurement Procedures IM 80J01A01 01E Select Protocol tab and click Save As Input name and descriptions of the protocol Click Save to save the protocol ...

Page 93: ...t loaded autofocus may not operate normally Set the viewer layout to ImageViewer Chart Click Rec to start measurement By clicking this button imaging by the condition set in 4 4 Imaging Setting and analyzing by the condition set in 4 5 Analysis Protocol Setting and 4 6 Object Recognizing Condition Setting are executed Image dataset and analysis dataset are saved in the folder designated in 4 2 Bas...

Page 94: ... of stopping click Stop button Acquired images are displayed in ImageViewer and statistic of feature data default is Count of wells which measurement is finished are displayed in WellTable during measurement After measurement switch to HDD mode and select double click the result measured above Stop button Select the result ImageViewer WellTable Statistics of feature data ...

Page 95: ...ageViewer Make button to status green check number of Thumbnail Chart to display and select wells in WellTable Scattergrams or histograms are displayed in ThumbnailChart For details of each window refer to following WellTables 5 6 5 WellTable Window ImageViewer 5 6 2 ImageViewer Window ThumbnailChart 5 6 4 ThumbnailChart Window ImageViewer WellTable ThumbnailChart ...

Page 96: ...ion by referring 4 5 Analysis Protocol Setting and 4 6 Object Recognizing Condition Setting and click Analyze In case of stopping analysis process click Stop button Output acquired images window snapshot of CQ1 Software or CSV data and so on in Output window For details refer to 5 5 4 Output Window ...

Page 97: ...ple measurement There are two operating modes CQ mode and HDD mode and the window display changes depending on the mode 1 CQ Mode Controls both CQ1 Main Unit and Utility Box and runs sample measurement Measurement data are saved in either the HDD of workstation or network drive 5 6 Viewer 5 4 Control Panel CQ Mode 5 7 Status Panel 5 3 Operation Mode Panel 5 2 Main Menu 5 8 Viewer Layout ...

Page 98: ...surement data or to retry image analysis under modified analysis conditions In HDD mode it is able to output data for report such as screen shot movie feature data and so on 5 6 Viewer 5 5 Control Panel HDD Mode 5 7 Status Panel 5 3 Operation Mode Panel 5 2 Main Menu 5 8 Viewer Layout ...

Page 99: ... Menu 5 2 1 File Menu 1 Save Measurement Protocol as Default Save the default measurement condition file Its channel setting and analysis conditions shall be applied at the next start 2 Save as Measurement Protocol Save the current measurement condition file ...

Page 100: ...lysis conditions are applied Fields to be imaged aren t applied if stage attachment and sample holder isn t matched In HDD mode only analysis conditions are applied 4 Save Contrast and Region View Setting This function is valid only in HDD mode Save Contrast Enhancement and Region setting of Tool function Refer to 5 6 9 Tool Function In case of not analyzed data only Contrast Enhancement setting i...

Page 101: ...to 5 10 Trimming Parameter for details 3 User Setting Open User Setting window to change user setting Please refer to 5 11 User Setting for details 4 Activation Setting Open Activation Setting window to manage license of CQ1 Software Please refer to 5 12 Activation Setting for details CAUTION Please do not operate Activation Setting window except when necessary such as updating CQ1 Software CQ1 So...

Page 102: ...sting window opens and changes to the external control mode that high order computer controls CQ1 Select this menu in case of operating CQ1 with external robot Click Cancel to quit external control 5 2 3 View Menu Viewers corresponding to each menu item are shown Please refer to 5 6 Viewer for details ...

Page 103: ... analysis information and output a compressed file of log and calibration file under CVSysRep folder on desktop The output data will be used for system maintenance service when error occures It takes a few minute to collect system information It is recommended to check to select date when error is occurred because the all date log data is quite huge ...

Page 104: ...ol panel display 2 CQ Mode Button Highlighted in the CQ mode but nothing happens by clicking this button Refer to 5 1 Outline 1 CQ Mode 3 HDD Mode Button Click to shift CQ1 Software to HDD mode Cannot click it during measurement Refer to 5 1 Outline 2 HDD Mode 4 Rec Button Click to start measurement after setting measurement conditions on the control panel 5 Pause Button Click to pause measurement...

Page 105: ...Click to shift CQ1 Software to CQ mode Cannot click it during analyzing measurement data Refer to 5 1 Outline 1 CQ Mode 3 HDD Mode Button Highlighted in the HDD mode but nothing happens by clicking this button Refer to 5 1 Outline 2 HDD Mode 4 Analyze Button To start analysis of measurement data select saved data on control panel set analysis conditions at Application window and Analysis window an...

Page 106: ...5 10 5 Functions of CQ1 Software IM 80J01A01 01E 5 4 Control Panel CQ Mode In CQ mode click left side tabs to set measurement conditions and so on ...

Page 107: ...ing window and analysis conditions specified at Application window and Analysis window Protocol window is to save or load Protocol and specify the folder name to save measurement data obtained under the Protocol 1 Save Setting Specify a storage folder to save measurement data To change the storage folder click button to show folder selection menu 1 2 3 4 5 6 7 ...

Page 108: ...ring input in Save Folder name For example in case of beginning measurement at April 1 2014 12 34 56 MeasuremenData folder name is 20140401T123456_Test Under MeasurementData folder sub folders are created as the following configuration There are folders not to be created by mesurement setting Note Please designate local drive D as data storage folder In case of designating other than local drive D...

Page 109: ...n result if Z 1 Analyses Folder to store analysis result Folder to be created by each analysis Log Map Reports Other files MeasurementResult ome tif Analysis_ Date FCS ICE Reports Other files Folder for each measurement data Folder name is set at Save Folder Name Folder to store FCS format data Folder to store ICE format data Snapshot png jpg Folder to store log data Folder to store Map imge Folde...

Page 110: ... in Workstation is shown By selecting a Protocol its details are shown Click x button on the upper right to close the Protocol detail window 5 Load Button Click this button after selecting a Protocol from the list to load it in the control panel Select protocol Show detail of protocol ...

Page 111: ...specified in the control panel XML file name is shown in the Name column of Protocol list Fill in the outline of protocol Contents can be confirm in 5 6 7 Information Window For details please refer to the section 7 Delete Button Select a Protocol from the list and click this button to show Delete Protocol File dialog Click YES to delete Protocol ...

Page 112: ... sample holder type Sample holder Description None CQ1 Software recognizes as if microplate is loaded and enables selection of Microplate Type Triple 35 mm dishes CQ1 Software recognizes a Triple 35 mm dishes sample holder is loaded in which up to three 35 mm dishes can be set and enables selection of Dish Type CAUTION Set dishes to all 3 positions Single 60 mm dish CQ1 Software recognizes a Singl...

Page 113: ... 5 Functions of CQ1 Software 5 17 IM 80J01A01 01E Selection of sample vessel type Microplate Selection of sample vessel type 35 mm dish Selection of sample vessel type 60 mm dish ...

Page 114: ...5 18 5 Functions of CQ1 Software IM 80J01A01 01E Selection of sample vessel type Slide glass Selection of sample vessel type Cover glass chamber ...

Page 115: ...ions of CQ1 Software 5 19 IM 80J01A01 01E By using Edit button at the bottom of Sample Vessel Type it is possible to modify sample shape and create customized sample type Example 35 mm dish Example slide glass ...

Page 116: ...played diagram in the window for dimensions to be input Measure Button Invalid when using slide glass Measure the bottom thickness of sample vessel This function can be used when 10x dry objective lens UPLSAPO10X2 is selected Laser AF Bottom Detection By checking the box surface to detect by laser autofocus is changed to lower surface of bottom plate of sample vessel This is checked when laser aut...

Page 117: ...ave modified parameters and finish editing Cancel Button Cancel modified parameter setting and finish editing Set Button Overwrite newly set parameters on selected sample vessel type and finish editing You cannot click this button when a key marked type is selected ...

Page 118: ...channels specified by Option menu Channel Setting menu Also by double clicking each channel Channel Setting window refer to 5 9 Channel Setting opens Name Description Number of imaging channel Excitation Light source information laser or transmission illumination Emission Emission filter information Acquisition Image acquisition conditions of camera such as exposure time or binning ...

Page 119: ...umber Specify binning condition of the camera Set binning number bigger and image resolution become lower image brightness become higher and data size become smaller Binning Number Description 1 Unuse binning function One camera pixel is saved as one pixel of image data 2 Combine adjacent 2X2 camera pixels and save as one pixel of image data 4 Combine adjacent 4X4 camera pixels and save as one pix...

Page 120: ...berration Correct chromatic aberration caused by difference of wavelength Default checked Laser Enhancement Check to enhance excitation power If checked value of excitation power turns red Note Normally excitation power is calibrated by power irradiated on sample If check this box the calibration is disabled and excitation laser is output by non calibrated laser power ...

Page 121: ... dragging wells when button is green the wells are selected for Rec light blue By right clicking or right dragging selection for Rec is canceled Same Area Check here to capture images of the same Area in all wells of microplate slide If unchecked imaging Areas can be selected individually for each well Select measurement area Refer to above All Select All wells are selected for Rec This botton is ...

Page 122: ... Stop preliminary imaging 3 Field Field selection One well in the sample holder is shown here within which location of current Field is shown By clicking within the well XY stage moves to the clicked point and capture an image which will be shown as preview at the ImageViewer XY coordinate X Y coordinate of current Field is shown for which the center of the well is the point of origin Field locati...

Page 123: ... at the current Field position at all channels selected at ImageViewer Z Mode Select to run Z Stack imaging or not while preview imaging Z Mode Description Capture one image at the Center of Z Stack Capture Slices images at StepHight interval between Top and Bottom of Z Stack Add Field Specify current imaging Field as the measurement location The measurement filed is shown in Map window of Viewer ...

Page 124: ...ofocus acquires image by designated channel and sets the Z position that maximum intensity is obtained as Z base position Microplate Laser Autofocus detecting surface slide glass Laser Autofocus detecting surface microplate dish cover glass chamber Image Autofocus detecting position Dish Slide Cover glass chamber In case that laser autofocus doesn t operete normally when using microplate dish or c...

Page 125: ...s searches for objects by moving imaging position in Z direction after Laser autofocus searched for the glass interface When checked Image Autofocus runs when imaging started by Start button at Map window in Viewer or when measurement started by PreRec button in Wells Laser AF Image AF Setting Click button to show Laser autofocus and Image autofocus setting window Run Laser AF at every field Run L...

Page 126: ...is changed when Range is fixed the other value parallel shifts by the same amount Slices is to set the number of Z Stack images to capture Step is the imaging interval step height of Z Stack Click left button to set suitable Step of selected objective lens Search Search button is to search for sample position through Z Stack imaging button is to set search conditions button is to input search resu...

Page 127: ...s mode time lapse imaging has a priority Time lapse imaging of speed and time length set at Fast Time lapse Condition is performed in each well and field Numbers of channel and Z slice are fixed to 1 When switching to this mode the selected channel remains and the others are removed automatically Also Chromatic Abberation becomes invalid automatically This mode is not correspond to on the fly anal...

Page 128: ...nds The specified time and acquisition time are shown on the right Duration Specify the total number of repetitions The total time is shown on the right Renewal Button Display total image number and total file volume By clicking this button total file volume and total image number are renewed If HDD space is not enough display warning sign Total image number Total file volume ...

Page 129: ...time lapse Set Interval and Duration Select row and click to delete the Variable Time lapse setting In case of variable interval time lapse Set Interval Duration and Variable Time lapse In this case Interval is applied until first time point to change interval In case of setting as above Time point Interval min 1 19 10 20 49 5 50 100 20 Note If larger value than Duration is designated for Time Poi...

Page 130: ...acquisition speed is determined by exposure binning and trimming If the value exceeding upper limit is input value is changed to actual upper limit automatically In case that FPS is unchecked acquisition speed according to exposure is set automatically Acquisition speed is limited by binning and trimming The calculated acquisition interval is shown on the right Note Imaging speed in Fast mode is u...

Page 131: ...of channel and Z slice are fixed to 1 So setting of this item has not special meaning Z Stack Multiple Z slice imaging by certain channel is performed in priority to multiple channel imaging Channel Multiple channel imaging by certain Z slice is performed in priority to multiple Z slice imaging This setting becomes available in case that Z Offset of all channels are set to 0 refer to 5 9 Channel S...

Page 132: ... 80J01A01 01E 5 4 4 Application Window Application window is for setting image analysis conditions 1 Execute Analysis When checked runs measurement and analysis at the same time and runs measurement only when unchecked 3 6 8 2 1 7 9 4 5 ...

Page 133: ... 5 Functions of CQ1 Software 5 37 IM 80J01A01 01E 2 Load Load Analysis Protocol Load Analysis Protocol User Window Load Analysis Protocol Application Window ...

Page 134: ...me defined by Object Detect Setting Details of Link Setting In this document Link means linkage among objects which is imaged by different channel Show details of Link Setting of the Analysis Protocol selected at or List of Object Detection Setting Show the Object Detect List of the Analysis Protocol selected at or Name Description Ch Show the channel for detection Name Show the name of object det...

Page 135: ...tions of CQ1 Software 5 39 IM 80J01A01 01E Cancel Close the window Other File Load Analysis Protocol from arbitrary protocol Delete Delete Analysis Protocol selected in Protocol selected in or can t be deleted ...

Page 136: ... in Nucleus Protocol for analyze nucleus and one category of dots 2 Color Dots in Nucleus Protocol for analyze nucleus and two categories of dots Nucleus and Cell body Protocol for analyze nucleus and cell body Nucleus and Cytoplasm Protocol for analyze nucleus and cytoplasm Dots in Cell body Protocol for analyze nucleus cell body and dots in cell body Dots in Nucleus Cytoplasm Protocol for analyz...

Page 137: ...IM 80J01A01 01E 3 Save Save the Analysis Protocol Name Define the name of Protocol which is also used as file name Description Input description of the Protocol Save Save the Protocol Cancel Close the window without saving the Protocol ...

Page 138: ...ction to create a single image by putting together pixels associated with the summation signals among identical pixels from multiple image data captured on the confocal plane in the Z axis direction It is able to display and analyze 3D objects as one image It is efficient if fluorescent intensity of sample is low Select Z slice images to analyze ALL Select all Z slices for analysis target Selected...

Page 139: ...analysis target Cancel Cancel Z slices range setting and close window 4 Time lapse Setting Select images of time point to analyze ALL Select all time point images for analysis target Selected Select images of time point for analysis target by following menu opened by Option button Click Option button and select time point for analysis target by following menu Total Show all acquired time points nu...

Page 140: ... is shown at the bottom right Selected Show selected time points for analysis target Total number of selected time points is shown at the bottom right Move time points between Unselected Selected OK Fix time points range setting for analysis target Cancel Cancel time points range setting and close window 6 Objective Detect Setting Show the list of current Object Detect Setting Name Description Lin...

Page 141: ...y the confocal channel is selectable However Phase Contrast channel is selected if no confocal channel is installed Add Add an Object Detect Setting to the end of the list Copy Copy selected Object Detect Setting and add to the end of the list However Link cannot be copied Remove Remove selected Object Detect Setting Raise up analysis order of selected Object Detect Setting by one step Step down a...

Page 142: ...low is called Sub node Link Setting Items Item Description Link Name Name of entire linked objects Link Tree Tree to show correlations among objects Root Node Top node Sub Node Other nodes Conditions of Sub Nodes Item Description Method Method for linking Adjacent to Root Node Included within Root Node Relationship Relationship with Root Node One to one relationship One to many relationship Root N...

Page 143: ...w Sub node cannot be added to an one to many link Object Detect cannot be linked to one to one more than once Object Detect cannot be linked to both one to one and one to many Name Set an entire name of all linked objects Link Tree Show currently set Link Tree Please refer to 7 Link Setting page 8 Link Setting 5 46 for items in the tree ...

Page 144: ...ppend Select the Object Detect to be linked The list of Object Detect Setting is shown ii Link Condition Select link condition Please refer to Conditions of Sub Nodes in 7 Link Setting page 5 46 iii OK Close window after confirming changes iv Cancel Cancel changes and close window i iv iii ii ...

Page 145: ...se window after confirming changes iii Cancel Cancel changes and close window i Append Select a reference Object Detect Setting from the list to be shown ii Link Condition Select link condition Please refer to Conditions of Sub Nodes in 7 Link Setting page 5 46 iii OK Close window after confirming changes iv Cancel Cancel changes and close window i iii ii i v iv iii ii ...

Page 146: ... IM 80J01A01 01E Remove Remove selected nodes except for Root Node Root Node cannot be removed Sub nodes of removed node are also removed altogether OK Close window after confirming link setting Cancel Cancel changes and close window ...

Page 147: ...preview red frame By left clicking or left dragging wells when button is the wells are selected for Analysis pink By right clicking or right dragging selection for Analysis is canceled Selection of Analysis Area Refer to above All Select All imaged wells are selected for Analysis Clear Selection for Analysis of all wells is canceled PreAnalyze Start preliminary analysis of selected wells for Analy...

Page 148: ...Object Window Exchange the Object Detect Algorism to be shown Reference Channel Reference Channel is to show the imaging channel for object detect ImageFilter Method MeanImage Apply averaging filter on images Mask Size is to set the range of averaging Binarization Method Threshold Apply binalyzation on images Set the minimum threshold value in Threshold Labeling Method DivideEachRegion Label each ...

Page 149: ...and volume for 3D analysis Edit Advanced Editor window opens For detail refer to 5 13 Advanced Editor Single Preview Analysis Apply selected object detection to designated Field in well selected for preview Stop Single Preview Analysis Preview Analysis Apply all object detections to designated Field in well selected for preview Stop Preview Analysis ...

Page 150: ...te of stage heater and its temperature Click Edit to set temperature ON Activate temperature control OFF Inactivate temperature control Setting Set temperature To apply click Set Cancel Cancel changes of temperature setting Set Apply changes of temperature setting 2 Camera Temperature Show current camera temperature 3 4 2 1 3 4 2 Stage Heater mounted model Stage Heater unmounted model ...

Page 151: ...ange Set Set XY stage speed 3 Laser Power Calibration Click Calibrate button to show Laser Power Calibration dialog and click Yes to proceed calibration but no calibration by clicking No Laser power calibration is performed to maintain excitation laser power irradiated to samples against Excitation Power set in Imaging window refer to 5 4 3 Imaging Window It takes a few minutes per 1 laser line to...

Page 152: ...lick left side tabs to set analysis conditions and to output data such as screenshot movie and so on If measurement data isn t selected only the Protocol tab is shown on control panel Open measurement data and other tabs are shown Measurement data isn t selected Measurement data is selected ...

Page 153: ...measurement data list which is saved in the folder selected by Measurement List is shown at Protocol window In detailed view more information is shown than brief view 4 5 6 7 1 3 9 16 17 18 8 2 Basical display mode Advanced display mode 10 11 12 13 14 15 Right click menu 19 20 21 22 23 ...

Page 154: ...l down list of previously selected folders While pull down list is shown selected folder can be deleted from the list by right click menu 2 Switch View Mode Switch brief detailed view mode of measurement data 3 Selection of Measurement Data Check to select measurement data Plural selection is available 4 Selection of Re measurement Data Re measurement means either review data or repeat image proce...

Page 155: ...ta selection check box Check to select feature data Plural selection is available Storage folder name of feature data Select re quantify data Re quantify data means to review or repeat image analysis on existing feature data This button will be circle marked when selected Delete Delete selected feature data available when more than 2 boxes are checked Read Viewer Show the re feature data circled f...

Page 156: ...9 Pen Button Show the text in Description of Protocol window of CQ mode Click this button to show text which can be overwritten Click outside of this window to close 10 Status Show the result status of measurement data It is able to confirm that measurement is finished or canceled and so on 11 Holder Show sample holder used for measurement 12 Sample Vessel Show sample vessel used for measurement 1...

Page 157: ...this button after selecting measurement data to show folder selection dialog Select either folder or drive to export data and click OK Data format of saving folder must be NTFS After export is compleated folder that measurement data is exported is opened Name of measurement data folder is the same as that of original measurement data folder 17 23 Delete Delete checked measurement data Click Yes fo...

Page 158: ...of clicking Read Viewer after selecting feature data Featured data is unselected Show images 20 Open Folder Open the folder that measurement data is saved by windows explorer 22 Export Custom Export measurement data with image processing Click this button after selecting measurement data to show folder selection dialog Data format of saving folder must be NTFS Select saving holder and click OK The...

Page 159: ... to export It is unselectable if there are no data corresponding to original data Items of Output Option Item Description Slices Export slice images MIP Export the MIP refer to page 5 42 image Sum Export the Sum refer to page 5 42 image Progress dialog Dialog to notice completion ...

Page 160: ...g Parameter Target Channel Number Select channel to perform arrangement It is available if Stitch is checked Overlap Parameter Pixel Designate the image size to perform arrangemetn It is available if Stitch is checked TargetImage of XY Position Designate the image to perform arrangement If images are acquired as 3D MIP and Sum is selectable It is available if Stitch is checked Help Tile Display he...

Page 161: ... 34 56 20150410T123456 3 In case that Tile is selected FieldIndex is 0001 4 In case that Time lapse Convert is selected continued number is assigned as timepoint 5 In case of MIP Z position is 000 In case of Sum Z position is SUM Cancel ExportSetting Window Close ExportSetting window without export Cancel Progress dialog Cancel export DataFolder MeasurementData Folder name is as following original...

Page 162: ...ow is to set image analysis conditions of existing measurement data Please refer to 5 4 4 Application Window for details 1 Analysis Folder Suffix Set suffix of analysis folder name 2 Load Load Analysis Protocol 3 6 8 2 1 7 9 4 5 Note Over 30 letters can t be input for Analysis Folder Suffix ...

Page 163: ...otocol 4 Z Analysis Setting Select Z slice images to analyze 5 Time lapse Setting Select images of time point to analyze 6 Object Detect Setting Show existing Object Detect Setting list 7 Edit Object Detect Setting 8 Link Setting Show current Link Setting 9 Edit Link Setting ...

Page 164: ...en button is green the wells are selected for Analysis pink By right clicking or right dragging selection for Analysis is canceled Selection of Analysis Area Refer to above All Select All imaged wells are selected for Analysis Clear Selection for Analysis of all wells is canceled PreAnalyze Start preliminary analysis of selected wells for Analysis PreAnalyze stop Stop preliminary analysis 2 Object...

Page 165: ...designated folder Report folder 1 Image Export Output Folder Click to open Report folder Report folder is generated in following path DataFolder MeasurementData Folder designated in Protocol tab Save Folder Name Analysis Reports Analysis_ Date Reports Folder designated in Protocol tab Save Setting Report folder Imaging and analyzing Only imaging ...

Page 166: ...tware Snapshot Window_ time and date of outputting snapshot 2 png jpg Movie Movie_W wellIndex F FieldIndex Z Z position 1 T start timepoint T end timepoint wmv mp4 Thumbnail Chart Chart window Snapshot Chart _ time and date of outputting snapshot 2 png jpg Movie Movie_W wellIndex F FieldIndex Z Z position 1 T start timepoint T end timepoint wmv mp4 1 In case of MIP Z position is MIP In case of Sum...

Page 167: ...ted number of image from Begin to End are picked up and converted to movie Frame Rate Designate frame rate of movie Movie Format Set output format of movie wmv is for WMV format and mp4 is for MP4 format Note In the case of time lapse data which is acquired by variable time lapse refer to page 5 32 movie is made by designated constant frame late without considering actual imaging Interval In made ...

Page 168: ...ch Gray Output each channel as gray image Output format is 8 bit Tiff Extension Option Select output format png PNG format jpg JPG format File Option Select output range Current Image Output images displayed in ImageViewer All Image Output projected images MIP Sum and so on of all fields of all wells and all timepoints File Name Prefix String Designate letters to add to file name Designated as Sam...

Page 169: ...ex F FieldIndex T timepoi nt Z Z position 2 C channelIndex png jpg 1 Name format is yyyyMMdd T HHmmss In case of April 10 2015 12 34 56 20150410T123456 2 In case of MIP Z position is 000 In case of Sum Z position is SUM 3D Viewer ImageJ Transfer image data to ImageJ and display as 3D image This function is available when ImageViewer setting is Slice refer to page 5 87 For detail please refer to ne...

Page 170: ... Set the configuration of 3D Viewer After setting click OK Z slice Designate the Z range for 3D projection Scale Factor Condensation 1 100 0 5 50 Set the ROI of 3D projection After setting click OK If you don t set ROI whole area of image is displayed in 3D Viewer Z slider Designate displayed Z position of preview window ...

Page 171: ...A01 01E 3D Viewer is displayed Zoom by wheel of mouse Rotate by left button drag of mouse Show the procedures to save snapshot of 3D Viewer From menu of 3D Viewer select View Take snapshot Set image resolution of snapshot After setting click OK ...

Page 172: ...1A01 01E Image of snapshot is displayed From menu of ImageJ select File Save as and save snapshot in arbitrary format TIFF PNG and so on Show the procedures to save 3D rotation movie From menu of 3D Viewer select View Record 360 deg rotation ...

Page 173: ... 5 Functions of CQ1 Software 5 77 IM 80J01A01 01E 3D rotation movie is displayed From menu of ImageJ select File Save as and save 3D rotation movie in arbitrary format TIFF AVI and so on ...

Page 174: ... wells in WellTable and Well is to cover currently selected wells wells in green area in WellTable Output Range Timepoint Select time point range to output as follows Individual Time points of currently selected range Each All analyzed time points data file is devided corresponding to each time point Total All analyzed time points all time points data is saved in one file DataFolder MeasurementDat...

Page 175: ... csv Total Output Range Well _Total_ output ting time and date 1 Name of measurement data folder Feature_Cell csv Statistic No sub folder Name of measurement data folder Statistics csv 1 Name format is yyyyMMdd T HHmmss In case of April 10 2015 12 34 56 20150410T123456 FCS Output Output data in FCS format Statistic can t be selected as output item ICE Output Output data in ICE format Statistic can...

Page 176: ...80J01A01 01E 5 6 Viewer In Viewer captured images image detected regions list of measurement and statistic of feature data and scattergram of measurement data are shown 5 6 1 Operation of Viewer Click tab to switch display of each window ...

Page 177: ... 5 Functions of CQ1 Software 5 81 IM 80J01A01 01E Drag tab to move the window ...

Page 178: ...5 82 5 Functions of CQ1 Software IM 80J01A01 01E Drag tab to square mark as following to add the tab to the window ...

Page 179: ... 5 Functions of CQ1 Software 5 83 IM 80J01A01 01E Drag tab to center arrow mark as following to allocate as new window by the window ...

Page 180: ...5 84 5 Functions of CQ1 Software IM 80J01A01 01E Drag tab to peripheral arrow mark as following to allocate as new window at end of Viewer ...

Page 181: ...ions of CQ1 Software 5 85 IM 80J01A01 01E Click x mark in upper right of each window to close selected window Windows can be set to default position by Viewer Layout button For detail refer to 5 8 Viewer Layout ...

Page 182: ...o it is able to zoom image by operation of mouse wheel This operation is same in 5 6 3 MAP Window Explanation of term Field and Area CQ1 can image not only one field but also multiple tiled fields In this section and next section 5 6 3 MAP Window single field is described Field and group of multiple tiled field is described Area Field 1 2 3 4 5 6 Area ...

Page 183: ...ield Area Image View Z Stack and Channel windows are to set Field to display and Area window is to set Area to display During measurement only the ongoing measurement is shown thus display settings cannot be changed Z Set Z position to display During measurement only the ongoing measurement is shown thus display settings cannot be changed Z Option Check MIP to display MIP image Check Sum to displa...

Page 184: ...5 88 5 Functions of CQ1 Software IM 80J01A01 01E 1 Image View Window It is possible to display multi channel images as one image by setting Time Field and Z ...

Page 185: ... 89 IM 80J01A01 01E 2 Z Stack Window Display tiled images from bottom to top in Z by setting Time and Field MIP and Sum check box is not available here Please refer to 6 View Option of 5 6 9 Tool Function page 5 120 about image size ...

Page 186: ...Q1 Software IM 80J01A01 01E 3 Channel Window Separately display images of each channel from a multi channel image by setting Time Field and Z Please refer to 6 View option of 5 6 9 Tool Function page 5 120 for display modes ...

Page 187: ... 5 Functions of CQ1 Software 5 91 IM 80J01A01 01E 4 Area Window Display image of Area by tile Change to Area from Field ...

Page 188: ...5 92 5 Functions of CQ1 Software IM 80J01A01 01E 5 Thumbnail Window Display thumbnail images of wells selected at WellTable window Can change Field and Z at each well ...

Page 189: ...below Objective lens selection Selected lens is highlighted Map clear Clear the map in display Area List Display Area list If there is more than one Area the leading number of Field is shown Unimagable Region Blue region in Map window can t be designated as imaging region because objective lens interferes with stage attachment sample holder and sample vessel 1 2 3 ...

Page 190: ...5 94 5 Functions of CQ1 Software IM 80J01A01 01E 1 Test Imaging Mode Capture test image of clicked Field at a single channel by one click Field to be imaged is shown as red square ...

Page 191: ...ap Mode Capture map image by selecting a region by left drug of mouse Selected region is shown in green Start Start map imaging MultiWell Start Start map imaging of multiple wells Stop Stop map imaging Clear Selection Clear selection of map imaging region ...

Page 192: ...of selected Area highlighted by orage frame at one channel and display on the map Start Color Capture test images of selected Area highlighted by orage frame by multiple channels under the same conditions for the measurement Stop Stop and above Remove it Remove selected Area Area numbers will be renewed Remove all Remove all Areas Define FOV Define the FOV Field of View at either 4 or 9 automatica...

Page 193: ...tware 5 97 IM 80J01A01 01E 5 6 4 ThumbnailChart Window ThumbnailChart has two data display modes 1 Scatter mode and 2 Histogram mode ThumbnailChart1 ThumbnailChart2 and ThumbnailChart3 have the same function 1 2 Y Axis X Axis ...

Page 194: ...ll in line or in horizontal priority Each Well Display Vertical Priority Display charts of each well longitudinally or in vertical priority Each Well Display Lattice Display charts of each well aligned in lattice Tile Number Shown when either Vertical Priority Display or Horizontal Priority Display is selected The number of charts to wrap lines can be specified Charts above the specified number ar...

Page 195: ...h density area in a chart Detail Dialog to select display range of X axis and Y axis is shown in which Min and Max display range and interval of X and Y axis can be set Logarithmic scale is selectable for each axis If enormously small value compared to axis size is set for Interval X or Interval Y the value isn t applied Object Name Select object detect name to plot X Axis Set the specified measur...

Page 196: ... If chart display is Well Merge above number of each well is shown If chart display is Each Well above number of each Field is shown ImageViewer shows clicked well or Field of the list Note In case that plural charts are shown region by mouse dragging is remained when new dragging region is selected in other charts In this case click at vacant area of the chart to erase a region ...

Page 197: ... Display Horizontal Priority Display charts of each well in line or in horizontal priority Each Well Display Vertical Priority Display charts of each well longitudinally or in vertical priority Each Well Display Lattice Display charts of each well aligned in lattice Tile Number Shown when either Vertical Priority Display or Horizontal Priority Display is selected The number of charts to wrap lines...

Page 198: ...t Min Color Set the color of the bar on a chart Detail Dialog to select display of X axis and Y axis is shown Min and Max display ranges and interval of X and Y axis can be set Logarithmic scale is selectable for each axis If enormously small value compared to axis size is set for Interval X or Interval Y the value isn t applied Object Name Select object detect name to plot X Axis Set specified me...

Page 199: ...y the list If chart display is Well Merge above number of each well is shown If chart display is Each Well above number of each Field is shown ImageViewer shows clicked well or Field of the list Note In case that plural charts are shown seleted region is remained when new region is selected in other charts In this case click at vacant area of the chart to erase a region ...

Page 200: ...ge will be shown by clicking a spot plotted in scattergram On the other hand a corresponding spot in scattergram will be cross marked by clicking an object By designating rectangle region in scattergram by mouse dragging the detected objects corresponding to spots in designated region in image will be shown ...

Page 201: ...e will be shown by clicking a bin in histogram On the other hand a corresponding bin in histogram will be marked by a vertical line by clicking an object By designateing region in histogram by mouse dragging the detected objects corresponding to bins in designated region in image will be shown ...

Page 202: ...table by left button dragging when this button becomes green by clicking By right button wells are unselected Wells become yellow during selecting Color of wells are designated automatically after selecting 3 Thumbnail Chart Selection By checking Thumbnail Chart 1 checkbox the chart of selected wells will be shown at ThumbnailChart1 window and so are the same with Thumbnail Chart 2 and Thumbnail C...

Page 203: ...r proportionally to the relative values Selectable measurement data are cell number per well and average and standard deviation of data described in 5 6 8 Measurement Data per well Cell number is expressed as Count average of each data is expressed as data name AVG and standard deviation is expressed as data name SD Please refer to 5 6 8 Measurement Data for details of measurement data ...

Page 204: ...e Window In FeatureTable window it is able to confirm measurement value of detected object such as cell granule and so on Please refer to 5 6 8 Measurement Data about shown items To confirm measurement value click object in ImageViewer or data in either ThumbnailChart ...

Page 205: ... that range of either axis is designated the data which is not shown in the chart is not shown at FeatureTable In case that the data of object selected in ImageViewer is shown in the chart the information of the object is shown in FeatureTable In case that the data of object selected in ImageViewer is NOT shown in the chart the information of the object is NOT shown in FeatureTable ...

Page 206: ...dow is to confirm information of measurement data This function is only for HDD mode In CQ1 mode display nothing 1 Comment Show Description of measurement data 2 Protocol Show Protocol of measurement data 3 Log Show log of measurement data 4 Report Show snap shot of measurement data 1 2 3 4 ...

Page 207: ...nce to upper left of image MaxX Max X axis µm In reference to upper left of image MinY Min Y axis µm In reference to upper left of image MaxY Max Y axis µm In reference to upper left of image Diameter Diameter µm Circumference Contour length µm Circularity Circularity SkeletonLength Length of line object µm JunctionCount Junction count of line object BranchCount Branch count of line object TotalIn...

Page 208: ...eft of Z 1 image MinY Min Y axis µm In reference to upper left of Z 1 image MaxY Max Y axis µm In reference to upper left of Z 1 image MinZ Min Z axis µm In reference to upper left of Z 1 image MaxZ Max Z axis µm In reference to upper left of Z 1 image Diameter Diameter µm Lateralarea Lateral area µm 2 Surfacearea Surface area µm 2 Sphericity Sphericity SkeletonLength Length of line object µm Junc...

Page 209: ... Main Y Coordinate Y coordinate of centroid µm of Main object Main Z Coordinate Z coordinate of centroid µm of Main object Sub1 Volume Volume µm 3 of Sub1 object Sub1 X Coordinate X coordinate of centroid µm of Sub1 object Sub1 Y Coordinate Y coordinate of centroid µm of Sub1 object Sub1 Z Coordinate Z coordinate of centroid µm of Sub1 object Sub2 Main COUNT Number of Sub2 object linked to Main ob...

Page 210: ... IM 80J01A01 01E 5 6 9 Tool Function Tool Function is to change the display style of image detected regions in ImageViewer or contrast of images Show detected area Detected area is shown in Image View Tab and Thumbnail Tab 1 2 3 4 6 5 ...

Page 211: ... ImageViewer and not shown when closed Fill All image detected regions will be colored if checked and only their outline will be colored if unchecked Stroke Outline of region gets thicker by moving this slider to the right The slider bar is not valid if Fill is checked Opacity Opacity of the color of image detected regions increase by sliding the bar to the left Multi Single Multi is to color dete...

Page 212: ...5 116 5 Functions of CQ1 Software IM 80J01A01 01E Color Select color of detected regions Invalid if Multi is selected Show Object ID Check to show ID of detected regions ...

Page 213: ... click of this button Images of current channel are shown in ImageViewer when the eye is open and not shown if closed Intensity Display Intensity information of displayed image is shown Intensity Range Max Min Intensity distribution Histogram Intensity range of displayed image Intensity range setting button Manual Contrast Range Contrast range can be manually adjusted by unchecking Auto Set the Mi...

Page 214: ...isplayed image in the current channel 3 Image Information Show detail information of the image displayed in ImageViewer 4 Auto Play Operate each slider bar in ImageViewer Slider bar operation Move to first image Stop auto play Start auto play Move to last image Auto play speed Designate speed of auto play from 1 slow 4 fast Looping auto play Slider bar selection Select slider to operate T Time Z Z...

Page 215: ...on with without of edge step and pitch of length changing can be changed and delete can be performed Text Box Add any text on images Edit text in ImageViewer By dragging position can be changed Also by right click menu color and font can be changed and delete can be performed Time Stamp Add time stamp on images By dragging in ImageViewer position can be changed Also by right click menu color and f...

Page 216: ...channel separately Show Caption Show captions such as channel number on each image Items to change display mode in ImageViewer when Thumbnail window at Imageviewer is open Each Well Display Horizontal Priority Display image of each well in line or in horizontal priority Each Well Display Vertical Priority Display image of each well longitudinally or in vertical priority Each Well Display Lattice D...

Page 217: ...Bar During measurement progress information is displayed in upper right of viewer Status Executing process is shown Progress Progress of measurement is shown Elapsed Time Elapsed time of measurement is shown Remaining Time Remaining time of measurement is shown Message ...

Page 218: ...t blue if Current Setting 1 Display of setting temperature become OFF if temperature control is off 4 Camera Status Camera Temperature Current value Set value is shown 5 Software Status Idle Idling Recording Under measurement Pause Waiting Status in between Pause and Stop buttons on Status Mode Panel under CQ Mode Preview Preview imaging is ongoing Analysis Analysis is ongoing Connecting Connectio...

Page 219: ...iewer Layout 1 ImageViewer Map Change viewer layout for image capturing 2 ImageViewer Chart Change viewer layout suitable for the analysis of each single cell by using charts 3 Chart Change viewer layout for chart analysis 4 Information Change viewer layout for viewing information of the CQ1 2 3 4 1 ...

Page 220: ...nnels are already selected 1 2 3 4 6 7 8 5 9 10 Note In case of Transmission Illumination Phase Contrast is mounted If PhaseContrast is selected phase contrast lens must be selected BrightField is unselectable In case of Transmission Illumination Bright Field Phase Contast is mounted If PhaseContrast is selected push the lever of transmission illumination back and select phase contrast lens If Bri...

Page 221: ...ed 8 Default Restore channel setting to the default 9 OK Close Channel Setting window with applying changes Analysis conditions are reset if channel setting is changed 10 Cancel Close Channel Setting window without applying changes Note If channel which is used in analysis setting the other channel is applied to the setting automatically ...

Page 222: ...trimming ratio of images 4 Save as trimmed image If checked trimmed images are saved If unchecked images without trimming is kept are saved In case of tiling images with stitching refer to page 5 64 uncheck the box 5 OK Close Trimming Parameter window with applying changes 6 Cancel Close Trimming Parameter window without applying changes 1 2 3 4 5 6 Note In case that correction of Chromatic Aberra...

Page 223: ...ollowing None Save all images in 1 folder By Field Create folders for each Field to save images by Field By Well Create folders for each well to save images by well By Time Point Create folders for each time point to save images 2 OK Close User Setting window with applying changes 3 Cancel Close User Setting window without applying changes 1 2 3 ...

Page 224: ...2 Message Message to explain status of license is shown 3 Days left Days until license expiration is shown 4 Activation code Activation code is shown 5 Remove license Select this radio button and click Continue to remove license 6 Version Version of CQ1 Software is shown 7 Enter Application Select this radio button and click Continue to return to Main window 8 Status Status of license is shown 9 C...

Page 225: ... 5 Functions of CQ1 Software 5 129 IM 80J01A01 01E 5 13 Advanced Editor In the Advanced Editor window object detecting algorism which was defined at Application window can be edited 1 2 3 4 ...

Page 226: ...ect detecting algorism which was defined at Application window can be edited Selection of Object Select the object detecting algorism Display of Image Click this button to show the image of reference channel used as input image for object detection in Analysis Viewer refer to following 2 ...

Page 227: ... Binarize all images with a fixed threshold calculated by Otu s method AdaptiveThreshold Binarize images with variable threshold according to image location ThresholdSigma Binarize images with fixed threshold calculated from the standard deviation of background image AdaptiveSigmaThresh old Binarize with dynamic thresholds calculated from the standard deviation of background image BinarizeTransfor...

Page 228: ...on ExcludeEdge Exclude edge of region SizeFilter Filter by size of region DiameterFilter Filter by diameter of region RoundnessFilter Filter by roundness of region MeanIntensityFilter Filter by mean intensity of region ROIFilter Filter by location of region Note MeanIntensity in this procedure contains background about 100 It is different from MeanIntensity output as measurement value Removal of I...

Page 229: ...o perform image processing until clicked method and to display the result at Analysis Viewer In CQ1 mode image processing is performable after test imaging by clicking PreRec of Imaging window In HDD mode performable consistently If Lost Reference exist at Input setting image processing is unperformable because algorism is not proper Previous Output Data of the last processing Nuclei 1 MeanImage D...

Page 230: ...5 134 5 Functions of CQ1 Software IM 80J01A01 01E 2 Analysis Viewer Analysis Viewer shows acquired image or processed image ...

Page 231: ...iii and iv processed image i Acquired Image Acquired image of reference channel to use as input image for object detection This image is displayed by contrast of black white ii Filtered Image Processed image after performing methods of ImageFilter group This image is displayed by contrast of black light blue ...

Page 232: ...nsform group This image is displayed by binary image objects are red and background is black Finally filtered image is overlapped iv Region Image Processed image after performing methods of Labeling or LabelTransform group Detected regions are displayed by individually colored outline Finally filtered image and binarized image are overlapped ...

Page 233: ...ll to display Field Set Field to display Z Set Z position of image to display Brightness Value Show the brightness value of pointed pixel Number of Detected Object Show number of detected object when region image is displayed Note When above setting is changed display of viewer returns to acquired image ...

Page 234: ...f CQ1 Software IM 80J01A01 01E 3 Analysis Viewer Setting Change the setting of Analysis Viewer Single View Mode Display acquired processed image by single view Dual View Mode Display acquired processed image by dual view ...

Page 235: ...After View Mode Display pre processed image above of window and post processed image below In this view it is convenient to confirm how image processing of the method works 4 OK Determine the editing and close the window If Lost Reference exists this button is unavailable 5 Cancel Cancel the editing and close the window ...

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Page 237: ...e space of system C disk drive larger than 30GB and data D disk drive larger than 1GB Defragmentation of hard disk drive Fragmentation becomes advanced by repeating write and deletes data Please do defragmentation of hard disk drive both data D and system C once in a half year at least Procedure Open the defragmentation window from property of disk Run Analyze disk and check the progress of fragme...

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Page 239: ...easurement is being processed Wait until the measurement ends It may take time for door opening in case that stage speed is set to low The stage does not run It might be that the door of CQ1 Main Unit is open Close it It might be that sub cover of CQ1 Main Unit is open Close it The micorplate can not be set on the stage It might be that the stage attachment for microplates is not set on the stage ...

Page 240: ...age attachment Set the cover glass chamber on the sample holder for cover glass chamber and set sample holder on the stage attachment The CQ1 Software does not run It might be that the CQ1 Software is run before Windows startup process ends Select CQ1 Software on Start Menu of Windows It might be that the connection between CQ1 Main Unit and Workstation is not built Connect them by the USB Cable C...

Page 241: ...t protocol Chamber temperature doesn t get to setting temperature Setting temperature might be low Confirm setting temperature and set proper temperature Is chamber temperature set over than room temperature 5 Confirm room temperature and set proper chamber temperature CO2 O2 concentrations of Gas Mixer don t get to setting concentration Remaining amount of each gas cylinder might not be enough Co...

Page 242: ...s to move over to left side of sample holder It might be that the door of CQ Main Unit opens Close it It might be that the key switch of Utility Box is OFF Turn the key switch ON Confirm ONLINE and Laser ON in status panel refer to 5 7 Status Panel Confirm that contrast setting is proper Refer to page 5 117 It might be that viewer status is turned to undisplayed Turn it to displayed status Refer t...

Page 243: ...xist in sample vessel Uncheck ImageAF Quality of map image is bad Zoom map image and map image is shown in original resolution Despite focusing by Search function images acquired by Rec are defocused In case that Rec position is far from Search position Z position that sample exist may different Perform focusing by Search at Rec position Waiting occurs during the measurement It might occur in case...

Page 244: ...r tilt Additionally acquire images of plural Fields in the well and compare their tilt If direction of object shift is different sample vessel might be distorted If direction of object shift is same CQ1 might have an abnormality of stage In this case please contact the dealer 1 Sample set part of Stage Attachment may be distorted If sample set part of Stage Attachment is distorted replacement is n...

Page 245: ...de effective Measurement and analysis can not be run simultaneously It might be that CQ mode is not selected Select CQ mode It might be that the check box of Execute Analysis in Application window is not checked Check the check box described above Lasers do not operate It might be that the key switch switch is not turned ON Turn it ON It might be that the remote interlock connector is not connecte...

Page 246: ...e Heater It might be caused by dust or water on the condensation reducer Clean the condensation reducer by referring 7 2 1 Cleaning Procedure of the Condensation Reducer Condensation occurs on room temperature sample when using Stage Heater Please warm the sample up to 30 and over 1 In the case of inquiring the dealer about trouble it may need to send system log file For outputting of log file ple...

Page 247: ... 7 Trouble Shooting 7 9 IM 80J01A01 01E Turn the key switch OFF then turn the power switch OFF Loosen 2 screws and remove sub cover Power Switch Key Switch ...

Page 248: ... remove screws from bath cover During temperature controlling water drops are formed on the downside of the bath cover Please be careful not to drop water inside the equipment when removing bath cover If water drops inside the equipment wipe it completely before turning CQ1 on Bath cover Water drop ...

Page 249: ...f there is water in the bath remove it by syringe and others Hold the tube and the block of condensation reducer slide them right and pull off the tube from the bath Rotate the block counterclockwise then pull off the block Condensation reducer ...

Page 250: ...01A01 01E If there is dust or water on the 2 holes on the edge of the block wipe them If there is dust or water in the slit wipe them After cleaning insert the edge of the block into the slit of the bath and rotate it clickwise Slit ...

Page 251: ...sert the tube to the bath hole Hold the tube and the block then slide them left to insert the tube to the hole until the end Set bath cover and tighten 4 screws In case that the screws can t be fastened properly adjust position of bath cover so that the screws are fastened properly ...

Page 252: ...7 14 7 Trouble Shooting IM 80J01A01 01E Set sub cover and tighten 2 screws ...

Page 253: ...set the range of Size Filter properly with visualization of Chart function It might be that viewer status for is turned to undisplayed Turn it to displayed status Refer to page 5 115 It might be that Area window is selected in ImageViewer Recognized result is not shown in Area window Select Image View window refer to page 5 88 It might be that Z position that sample doesn t exist is shown by viewe...

Page 254: ...o match measurement condition Where are plural analysis results of one measurement data by different analysis protocols Analysis result list is shown by clicking refer to page 5 59 3D Viewer of Image J freezes It might be that the size of image is too large Set the value of Scaling smaller It might be that the display driver of WS or PC is unproper It might occure when OS is upgraded Please inquir...

Page 255: ...lem Probable Cause Corrective Action Image Viewer window and or Thumbnail Chart window disappear These windows can be shown by Menu View menu Access to hard disk drive becomes slower It might be that the free space on the HDD is small Keep the free space by deleting unused files It is recommended that Windows command Defrag is run after deleting unused files While image acquisition and analysis ar...

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Page 257: ...61 640 nm L41 Wavelength 405 488 561 640 nm Dichroic mirror M1 DM 405 488 561 640 nm Transmission Illumination P Phase Contrast T Bright Field Phase Contrast N Stage Heater H With Stage Heater N Without Stage Heater Stage Attachment A1 All Wells Imaging Type A2 Chambered Type Workstation W1 Standard Workstation Language Shipping Form AC Cord J Japanese with Monitor with AC Cord N Japanese without ...

Page 258: ...king Distance NA 0 45 LUCPLFLN20X L040L 40x Long Working Distance NA 0 60 LUCPLFLN40X L010P 10x Phase Contrast NA 0 30 UPLFLN10X2PH L020P 20x Phase Contrast NA 0 45 LUCPLFLN20XPH N0000 Holder Without Objective Lens Custom Order Z Custom Order 8 1 3 Emission Filter Model Code Suffix Code Option Description CQ1FLT D Dummy code Additional specification code EM emission filter Position 1 9 Selling sep...

Page 259: ...ype SAT2 Chambered Type Sample Holder HDA01 For Triple 35 mm Dishes HDA02 For Single 60 mm Dish HDA03 For Slide Glass ISO 8037 1 HDA04 For Cover Glass Chamber Sealing Parts for Sample Holder CB601 60 mm Dish Ring CB602 Rubber for 60 mm Dish CBT01 Holding Plate for CGC CBT02 Rubber for CGC IWAKI CBT03 Rubber for CGC NUNC Lab Tek I CBT04 Rubber for CGC NUNC Lab Tek II CBT05 Rubber for CGC MATSUNAMI ...

Page 260: ...ter 41 3 kg Without Stage Heater 40 kg With Stage Heater 43 3 kg Noise level 46 dB max A weighted sound pressure level is less than 70dB Utility Box External dimensions W 275 mm x D 432 mm x H 298 mm Mass 18 kg Gas Mixer External dimensions W 170 mm x D 260 mm x H 280 mm Mass 5 2 kg Workstation for measurement External dimensions W 172 mm x D 417 mm x H 298 mm Mass 14 kg Measurement software Set m...

Page 261: ...ing slideglass sample holder up to 4 slide glasses are applicable in one experiment 4 Cover Glass Chamber By using cover glass chamber holder one cover glass chambdr is applicable in one experiment 1 If 384 well microplate is measured by 2x objective lens multiple wells are measured by one FOV Therefore in this case measurement is inhibited Warning is displayed If 1536 well microplate is measured ...

Page 262: ... measurement control and laboratory use EMC requirements Part 1 General requirements Performance Criteria Performance Criteria ESD IEC 61000 4 2 B Radiated electromagnetic field IEC 61000 4 3 A EFT Burst IEC 61000 4 4 B Surge IEC 61000 4 5 B Conducted disturbance IEC 61000 4 6 A Power freq magnetic field IEC 61000 4 8 A Voltage dips IEC 61000 4 11 B 0 5 1cycle 0 C 25 cycles 70 Short interruptions ...

Page 263: ...000 3 2 Class A Electromagnetic compatibility EMC Part 3 2 Limits Limits for harmonic current emissions equipment input current 16 A per phase EN IEC 61000 3 3 Electromagnetic compatibility EMC Part 3 3 Limits Limitation of voltage changes voltage fluctuations and flicker in public low voltage supply systems for equipment with rated current 16 A per phase and not subject to conditional connection ...

Page 264: ...or measurement control and laboratory use Part 1 General requirements EN IEC 60825 1 Safety of laser products Part 1 Equipment classification and requirements Environmental Standard EN 50581 Laser Safety Standard EN IEC 60825 1 Safety of laser products Part 1 Equipment classification and requirements FDA Laser Safety Regulation Information of Inner laser parts 405 nm laser for excitation AEL laser...

Page 265: ...B Maximum output power 50 mW Wavelength 561 nm Pulse duration repeating frequency Continuous mode Angular subtense 0 1 rad 640 nm laser for excitation AEL laser safty class 3B Maximum output power 100 mW Wavelength 640 nm Pulse duration repeating frequency Continuous mode Angular subtense 0 1 rad Laser for autofocus AEL laser safty class 3R Maximum output power 2 5 mW Wavelength 785 nm Pulse durat...

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Page 267: ... modifications made by the user Failures or damage caused by transportation movement dropping or the like occurring after purchase Failures or damage caused by a fire natural disaster earthquake storm flood lightning and so on salt damage gas damage and abnormal voltage Failures caused by the following processing on the workstation Install patches Windows Update service pack security patch to upda...

Page 268: ...nter Measurement Business HQ Yokogawa Electric Corporation 2 3 Hokuyodai Kanazawa shi Ishikawa 920 0177 Japan Phone 81 76 258 7028 Fax 81 76 258 7029 E mail CSU_livecell_imaging cs jp yokogawa com Web site http www yokogawa com scanner Please contact the dealer inquiries about this product ...

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