ThermoFisher Scientific Applied Biosystems Veriti 4375305, User Manual, Page: 45 / 106

Loading Samples in 96-well instruments
This section describes how to load samples in:
• Tubes with attached caps
• 96-well reaction plates
• 96-well tray/retainer assemblies
Loading tubes with attached caps
There is no need to use mineral oil or glycerine in the sample wells or as a vapor barrier over the PCR
reaction mixture in the tubes. The MicroAmp
™
Reaction Tubes fit tightly in the wells, and the heated
cover exerts an even pressure on all tubes, eliminating condensation on the tubes.
1.
Set the 96-well tray on a MicroAmp
™
Splash-Free 96-Well Base.
2.
Place the reaction tubes in the tray.
3.
Pipette the samples into the reaction tubes.
4.
Cap the tubes.
5.
See “Placing the sample tray or plate onto the sample block(s)” on page 46 .
Loading the reaction plate
1.
Place the reaction plate on a MicroAmp
™
Splash-Free 96-Well Base.
2.
Pipette the samples into the sample wells.
3.
Cap the plates using the MicroAmp
™
8-Cap Strips, or cover the plates with MicroAmp
™
Clear
Adhesive Film.
4.
See “Placing the sample tray or plate onto the sample block(s)” on page 46 .
Loading tubes without caps
To load the 96-well tray/retainer:
1.
Place the tray on the splash-free 96-well base.
2.
Load tubes onto the tray using either single tubes or the 8-tube strips.
3.
Place the retainer over the tubes.
4.
Pipette the sample into the tubes.
5.
Cap the tubes using a MicroAmp
™
8-Cap Strip.
6.
See “Placing the sample tray or plate onto the sample block(s)” on page 46 .
Chapter 3
Performing a run
Loading samples into the instrument
3
Veriti ™ Thermal Cycler User Guide
45