c.
Repeat for any other field.
3.
Select
Done
to close the editor.
Editing ramp rates
You can edit the rate at which the instrument ramps to the temperature for the step. The default ramp
rate is 100%.
Note:
You cannot edit ramp rates for methods created using the Convert Method wizard (see “Using
).
1.
Touch a step to select it.
2.
Touch
Options
4
Ramp Rate
.
The field displaying time changes to display the ramp rate.
3.
Select the ramp field, then enter the desired ramp rate.
Ramp rates from 0.1 to 100% are valid.
4.
Select
Done
to close the editor.
The ramp rate symbol appears next to any step with a ramp rate less than 100%.
Creating an AutoDelta step
An AutoDelta step allows you to increase or decrease the temperature and/or change the duration of
the cycle during a cycling stage.
One use for an AutoDelta step is for touchdown PCR, a method in which the earliest cycles have a
high annealing temperature, followed by cycles where the annealing temperature decreases slightly for
each cycle. Touchdown PCR increases the specificity of the PCR products. The high initial temperature
favors more specific annealing between the primer and the template. Because these fragments are
further amplified during subsequent cycles at lower temperatures, they outcompete the nonspecific
sequences to which the primers bind at lower temperatures.
Note:
AutoDelta steps are allowed only in cycling stages.
To create an AutoDelta step:
1.
Select a step, then
Options.
The options menu appears:
Chapter 3
Performing a run
Editing details
3
52
Veriti
™
Thermal Cycler User Guide
