ThermoFisher Scientific Applied Biosystems Veriti 4375305, User Manual, Page: 49 / 106

6.
Select (Folder) to choose a folder in which to save the run method.
• To save the run method so that all users have access to it, select
Shared .
• To create a new folder for the run method, select New Folder at the bottom of the list, enter a
name of up to 12 characters (no spaces), for the new folder, then select Done .
• To save the run method to the USB drive, select
USB (available only when a USB drive is
inserted in the instrument).
7.
Select Reaction Volume to enter the reaction volume. (You can change this volume when you
perform a run with this run method.)
8.
Set the cover temperature, if any:
•
Cover Temperature to enter the temperature for the heated cover.
or
• Do Not Heat Cover .
You can change this temperature when you perform a run with this run method.
9.
Select Notes , then enter any notes for the method.
10.
Select Save & Exit when you are done.
The Browse Run Methods screen is displayed.
Editing details
• This section describes how to edit a run method by:
• Adding a stage (“Adding a stage”
on page 49 )
• Adding a step (“Adding a step” on page 50 )
• Editing a temperature or a time (“Editing temperature and time”
on page 51 )
• Editing a ramp rate (“Editing ramp rates”
on page 52 )
• Creating an AutoDelta step (“Creating an AutoDelta step”
on page 52 )
• Adding a pause (“Adding a pause” on page 54 )
• Creating a step to program different temperatures on the VeriFlex ™
Blocks (“Creating a VeriFlex
™
step” on page 55 ) (Available only on 96-well instruments.)
Adding a stage
The two types of run method stages are:
•
Cycling – The steps in a cycling stage are repeated a specified number of times. In general, the
steps in a cycling stage are template denaturation, primer annealing, and primer extension.
•
Holding – The steps in a holding stage are not repeated.
To add a stage:
Chapter 3
Performing a run
Editing details
3
Veriti ™ Thermal Cycler User Guide
49