ThermoFisher Scientific Applied Biosystems Veriti 4375305, User Manual, Page: 93 / 106

AmpliCycle ™ Seq Run Method
Use this run method to achieve clean sequence ladders from femtomole quantities of template. The
cycle sequencing process and the benefits of AmpliTaq
™
DNA Polymerase, CS, are described in the
product insert for the AmpliCycle
™
Sequencing Kit (PN N8080175).
AmpliTaq Gold
™
DNA Polymerase Run Method
Use this run method for hot-start PCR in place of labor-intensive methods such as manual hot-start
or wax-bead-mediated hot-start techniques. The hot-start technique helps to minimize the formation of
primer-dimers or nonspecific products, thereby increasing specificity and sensitivity of PCR. This run
method specifies a pre-PCR heat step for activation of AmpliTaq Gold
™
DNA Polymerase.
AmpliTaq Gold ™ Fast PCR Run Method
Use this run method for PCR with AmpliTaq Gold
™
Fast PCR Master Mix, UP to produce optimal yield
and specificity for a 500 bp amplicon. This run method is optimized to generate template for DNA
sequencing reactions and is only available on 96-well instruments.
Note:
By default, the annealing temperature is set at 62°C. Replace this temperature with the melting
temperature of the primer (see “Calculating melting temperatures” on page 69 ).
Appendix E
Predefined run methods
Predefined run methods
E
Veriti ™ Thermal Cycler User Guide
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