1
Introduction
Specifications
Thermo Scientific
Accela 600 and 1250 Pump Hardware Manual
9
Table 6.
Accela 1250 Pump operating specifications
Specification
Description
Backpressure range
0 to 1250 bar (18 130 psi)
Flow rate range
1 to 2000 μL/min
Pressure signal accuracy
±1% absolute at calibration point
±5% full range
Residual pulsation
< 1.5 bar standard deviation full working range
Typical < 0.5 bar (7 psi) amplitude
Flow rate accuracy
±0.5% or 1 μL/min, whichever is greater
Gradient composition accuracy
±0.5% absolute from 1 to 1500 μL/min
± 2% absolute from 1500 to 2000 μL/min
Gradient resolution
1% from 0 to 100%
Gradient composition range
0 to 100% from up to four independent feed
channels employing two of them at the same time
Delay volume
70 μL (LDA) + 65 μL (static mixer)
Firmware version (November 2010)
23.67 f
Table 7.
Degasser operating specifications
Specification
Description
Channels
4
Degassing technology
Gas permeation through a fluoropolymer
membrane
Maximum flow rate
10 mL/min
Degassing capacity
~2 ppm residual gas at 1 mL/min
Delay volume per channel
~480 μL
Wetted surfaces
PEEK, glass-filled polytetrafluoroethylene (PTFE),
trade name Teflon AF
Table 8.
Electronic specifications
Specification
Description
Communications
19 200 baud, 1 start, 8 data, 1 stop, no parity and
no handshaking, proprietary protocol/USB 1.0
Audio output
Piezo buzzer