Thermo Scientific Accela 1250, Hardware Manual

Page: 102 / 170

5
Maintenance
Maintenance Schedule
78 Accela 600 and 1250 Pump Hardware Manual Thermo Scientific
Maintenance Schedule
To ensure optimal performance of the pump, perform preventive maintenance at regularly
scheduled intervals. Perform corrective maintenance when you observe poor performance or
when you have been instructed to do so by a Thermo Fisher Scientific field service
representative.
Table 12 lists the recommended maintenance schedule for the pump beginning with the most
frequently performed procedures.
Tip With time, acetonitrile mobile phases coat the sapphire check valve seats with an
aliphatic amine residue that can cause the check valves to stick. To prevent residue
buildup, periodically pump HPLC-grade methanol or isopropanol (IPA) through the
system as described in
“Flushing the LDA” on page 104 . If you are using a buffered
mobile phase, pump a non-buffered solvent that is miscible with the buffered mobile
phase through the system before pumping methanol or IPA through the system.
Table 12. Maintenance frequency
Procedure Frequency Page
Flushing the LDA If the LC pump does not have the
optional seal wash pump, flush the LDA
once per day if you use buffered mobile
phases and once per month if you use
mobile phases that contain acetonitrile. 104
Flushing the Piston Guide Bushings Once per day if you use buffered mobile
phases and you do not have the seal
wash pump installed 105
Replacing the Primary Piston Seals Every 6 to 12 months depending on
usage 97
Cleaning the Check Valves Every 6 to 12 months if you use buffered
mobile phases 103
Cleaning the Pistons Every 6 to 12 months depending on
usage 103
Cleaning the Instrument Casing As needed 104
Removing Air from the Piston
Chambers As needed 106
Cleaning the Displacement Chambers
and the Inlet Module As needed 107
Replacing the Fuses As needed 108
Replacing the Secondary Piston Seals As needed 101