5
Maintenance
Cleaning the Instrument Casing
104
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
Cleaning the Instrument Casing
If the pump enclosure becomes dirty, clean it with a soft cloth lightly moistened with a mild
detergent solution. Do not use any type of abrasive pad, scouring powder, or solvent.
Flushing the LDA
To maintain optimal performance of the LDA, do the following:
• If you use buffered mobile phases, flush the pump’s LDA daily with a non-buffered
mobile phase. Flushing the LDA helps prevent particulates from sticking in the check
valves, which might cause pressure problems.
• If you use mobile phases that contain acetonitrile, flush the LDA monthly with
HPLC-grade methanol or IPA. This removes the aliphatic amine residue that can build
up on the sapphire check valve seats and cause the check valves to stick.
To flush a buffered aqueous mobile phase out of the LDA
1. Set up the system to pump LC/MS-grade deionized water through the LDA for
10 minutes.
2. Pump LC/MS-grade methanol or IPA through the LDA for 10 minutes.
3. Pump LC/MS-grade deionized water through the LDA for an additional 10 minutes.
To flush buffered acetonitrile mobile phases out of the LDA
1. Set up the system to pump a non-buffered solvent that is miscible with the buffered
mobile phase through the LDA for 10 minutes.
2. Pump LC/MS-grade methanol or IPA through the LDA for an additional 10 minutes.
To flush non-buffered acetonitrile mobile phases out of the LDA
Pump LC/MS-grade methanol or IPA through the LDA for10 minutes.
CAUTION
Wear safety gloves when handling solvents.
