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Thermo Scientific

Accela 600 and 1250 Pump Hardware Manual

Introduction

This chapter introduces the current versions of the Accela 600 Pump (see

Figure 1

) and the

Accela 1250 Pump.

Note

For information about earlier versions of the Accela pumps, see

Appendix A,

“Legacy Hardware Components.”

Note

In this manual, the term Accela pump refers to the Accela 600 Pump and the

Accela 1250 Pump. For information about the Accela Pump, refer to the

Accela Pump

Hardware Manual

Contents

•

Functional Description

•

Hardware Components

•

Status LEDs

•

Specifications

Summary of Contents for Accela 1250

Page 1: ...Thermo Accela 600 Pump and 1250 Pump Hardware Manual 60157 97010 Revision J December 2013 ...

Page 2: ...peration This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document...

Page 3: ...d by Thermo Fisher Scientific To ensure continued compliance with EMC and safety standards replacement parts and additional components options and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives Accela 600 Pump EMC Directive 2004 108 EC EMC compliance has been evaluated by TÜV Rheinland of North America Inc Low Voltage Safety Compliance This devic...

Page 4: ...tage Directive 2006 95 EC and the following harmonized standards EN 61010 1 2001 IEC 61010 1 2002 UL 61010A 1 2004 CAN CSA 22 2 61010 1 2004 EN 55011 2007 EN 61000 4 5 2005 EN 61000 3 2 2006 EN 61000 4 6 2007 EN 61000 3 3 1995 A1 2001 A2 2005 EN 61000 4 11 2004 EN 61000 4 2 1995 A1 1999 A2 2001 EN 61326 1 2006 EN 61000 4 3 2006 FCC Class A CFR 47 Part 15 2009 EN 61000 4 4 2004 ...

Page 5: ...s to the system and part replacement Accordingly order replacement parts from Thermo Fisher Scientific or one of its authorized representatives Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For manufacturi...

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Page 7: ... Directives and the recyclers in your country WEEE Konformität Dieses Produkt muss die EU Waste Electrical Electronic Equipment WEEE Richtlinie 2002 96 EC erfüllen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder ents...

Page 8: ... compagnies de recyclage dans chaque état membre de l union européenne et ce produit devrait être collecté ou recyclé par celles ci Davantage d informations sur la conformité de Thermo Fisher Scientific à ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont disponi...

Page 9: ...es Gefahr durch entzündbare Substanzen Beachten Sie die einschlägigen Vorsichtsmaßnahmen wenn Sie das System in Gegenwart von entzündbaren Substanzen betreiben Peligro por sustancias inflamables Tenga mucho cuidado cuando utilice el sistema cerca de sustancias inflamables Danger lié aux substances inflammables agir avec précaution lors de l utilisation du système en présence de substances inflamma...

Page 10: ...s away from this area Quetschgefahr Halten Sie Ihre Hände von diesem Bereich fern Puntos de pinzamiento Mantenga las manos apartadas de esta área Risque de pincement éloigner les mains de cette zone Heavy objects Never lift or move the instrument by yourself you can suffer personal injury or damage the equipment For specific lifting instructions refer to the instrument manual Schweres Objekt Beweg...

Page 11: ...れる前に加熱した部品を冷ましてください热表面待高温部件冷却之后再进行维修 Flammable substances hazard Use care when operating the system in the presence of flammable substances 可燃性物質の危険性可燃性物質があるところでシステムを作動させる場合は十分注意してください易燃物危险在有易燃物质的场地操作该系统时务必小心谨慎 Risk of eye injury Eye injury could occur from splattered chemicals airborne particles or sharp objects Sharp objects that customers might install in the instrument include fused si...

Page 12: ...th the object 鋭利な物体物体との身体的接触を避けてください锋利物体避免直接接触锋利的物体 Pinch point Keep hands away from this area ピンチポイントこの部分には手を挟まれないようにしてください夹点勿将手放在此部位 Heavy objects Never lift or move the instrument by yourself you can suffer personal injury or damage the equipment For specific lifting instructions refer to the instrument manual 重量物 1 人で機器を持ち上げたり移動しないでください身体傷害を負ったり機器を損傷するおそれがあります具体的な持ち上げ方法については機器のマニュア...

Page 13: ... Specifications 8 Chapter 2 Accela 600 Pump or Accela 1250 Pump Installation 11 Accela Pump Installation Checklist 12 Shipment Inspection 13 Benchtop Setup 13 Layout for an Accela LC System with an Accela Autosampler 13 Layout for an Accela LC System with an Accela Open Autosampler 15 Back Panel Connections 17 Connecting the Power Cable 18 Connecting the Pump to the Data System Computer 19 Connect...

Page 14: ...ument Configuration 71 Adding Pump 2 to the Instrument Configuration 74 Specifying the Leak Sensor Settings in the Xcalibur Data System 75 Chapter 5 Maintenance 77 Maintenance Schedule 78 Accessing Disassembling and Reassembling the LDA 79 Removing the LDA from the Pump 79 Disassembling the LDA 85 Reassembling the LDA 89 Reattaching the LDA to the Pump 92 Replacing the Primary Piston Seals 97 Repl...

Page 15: ...es 127 Accela 600 Pump Accessory Kit 129 Accela 1250 Pump Accessory Kit 130 Accela System Kit 131 Accela 600 Pump and Accela 1250 Pump Inlet Tubing Kit 133 Compression Fittings for High Pressure Connections 133 Tools 133 Seal Wash Pump Kit 134 Dynamic Mixer 135 Leak Sensor Kit 135 Appendix A Legacy Hardware Components 137 Proportioning Valves 137 Fittings and Tubing 139 Low Pressure Solvent Lines ...

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Page 17: ...rmo Scientific Accela 600 Pump and the Accela 1250 Pump You can operate these Accela pumps from a computer with an installation of either the ChromQuest or Thermo Xcalibur data system Contents Related Documentation Safety and Special Notices Safety Information Environmental Conditions Good Laboratory Practices Keeping Good Records Contacting Us ...

Page 18: ...et up your Accela LC system and interconnect the system modules Accela Pump Help for the ChromQuest data system Provides instructions on how to control the Accela pumps from the ChromQuest data system Thermo Pump Help for LC Devices Provides instructions on how to configure the pumps in the Thermo Foundation platform and control the pumps from the Xcalibur data system For Thermo Scientific softwar...

Page 19: ...ccela product line as follows a In the Model list select Accela b In the Document Type list select Manual c Click Submit Search 6 On the Documents page click the link to the right of Download to view the PDF 7 Click the Save a Copy icon browse to an appropriate folder and then click Save to save a copy of the document ...

Page 20: ...tain information that is critical for optimal performance of the system Note Highlights information of general interest Tip Highlights helpful information that can make a task easier CAUTION Highlights hazards to humans property or the environment Each CAUTION notice is accompanied by an appropriate CAUTION symbol CAUTION Highlights electric shock hazards to humans Each electric shock notice is ac...

Page 21: ...n provided by the equipment CAUTION Do not service any part when operating the instrument Do not remove the front panel when the instrument is running Do not run the instrument without the front panel CAUTION The instrument contains voltage lines Switch off the power and disconnect the power cable prior to servicing the instrument There is no need to open the pump enclosure as all user serviceable...

Page 22: ...to the environment do the following Keep good records Read the manufacturers MSDSs for the chemicals you use in your laboratory Remove particulate matter from your samples before injecting them into the liquid chromatograph Use HPLC grade or LC MS grade solvents Connect the drainage tubes from the pump autosampler and detector to an appropriate waste receptacle Dispose of solvents as specified by ...

Page 23: ...ar with the toxicity data and potential hazards associated with all chemicals by referring to the manufacturers MSDSs Solvent Requirements Use LC MS grade solvents that are free of particulates Choose a mobile phase that is compatible with the sample and column you have selected for your separation Be aware that some solvents are corrosive to stainless steel Solvent Disposal Make sure you have a s...

Page 24: ...ort landing support html To copy manuals from the Internet Go to mssupport thermo com agree to the Terms and Conditions and then click Customer Manuals in the left margin of the window To suggest changes to the documentation or to the Help Fill out a reader survey online at www surveymonkey com s PQM6P62 or Click the link below Send an e mail message to the Technical Publications Editor at techpub...

Page 25: ...a 1250 Pump Note For information about earlier versions of the Accela pumps see Appendix A Legacy Hardware Components Note In this manual the term Accela pump refers to the Accela 600 Pump and the Accela 1250 Pump For information about the Accela Pump refer to the Accela Pump Hardware Manual Contents Functional Description Hardware Components Status LEDs Specifications ...

Page 26: ...um programmable flow rate for both pumps is 1 μL min Power Communication Run Degas Power switch Tip For gradient applications Thermo Fisher Scientific recommends that you use a flow rate equal to or greater than twice the gradient delay volume of the pump s liquid displacement assembly LDA Table 2 Flow rate range and maximum operating pressure Pump Flow rate range for optimal performance Maximum o...

Page 27: ...a 600 Pump and the Accela 1250 Pump are benchtop units that you can integrate with other Accela instruments Hardware Components The Accela 600 Pump and the Accela 1250 Pump have these major components the vacuum degasser assembly the LDA a 65 μL static mixer an optional dynamic mixer an optional seal wash pump and an optional leak sensor The status LEDs on the left door of the pump enclosure displ...

Page 28: ... HPLC and must be kept to an absolute minimum for best performance The solvent degassing system consists of four independent channels that are maintained at a constant vacuum of approximately 50 mm Hg absolute Each channel has a volume of less than 500 μL which provides superior degassing efficiency capable of limiting dissolved oxygen to a level of only 2 ppm By varying its speed the vacuum pump ...

Page 29: ...e is open the mobile phase exits the LDA from the left side of the outlet module When the purge valve is closed the mobile phase flows out the front of the LDA assembly and into the high pressure tubing that connects the LDA to the static mixer Hardware Upgrades You can install these hardware upgrades a dynamic mixer a seal wash pump and a leak sensor Dynamic Mixer The 600 and 1250 pumps ship with...

Page 30: ...quid fraction evaporates The abrasion caused by this precipitate can scratch the piston and shorten the life of the piston seals With the seal wash pump option a large reservoir above the pump continuously feeds the pump and flushes the piston guide bushings to prevent the precipitation of salt crystals behind the primary piston seals For information about installing the seal wash pump see Seal Wa...

Page 31: ...en A pump program is downloading from the data system computer Run Amber The power is switched on but the pump pistons are idle producing no flow Flashing amber A firmware download is in progress or an error condition has occurred Green The pump pistons are moving but a pump program is not running This can occur when the pump is under direct control or a pump program has ended Flashing green The p...

Page 32: ...cification Description Width 36 0 cm 14 in Depth 45 7 cm 18 in Height 18 0 cm 7 1 in Weight 19 5 kg 43 lb Table 5 Accela 600 Pump operating specifications Specification Description Backpressure range 0 to 600 bar 8702 psi Flow rate rangea a The minimum flow rate for firmware versions earlier than 23 67 e is 10 μL min 1 to 5000 μL min Residual pulsation 2 bar 29 psi amplitude Flow rate accuracy 0 5...

Page 33: ... Gradient resolution 1 from 0 to 100 Gradient composition range 0 to 100 from up to four independent feed channels employing two of them at the same time Delay volume 70 μL LDA 65 μL static mixer Firmware version November 2010 23 67 f Table 7 Degasser operating specifications Specification Description Channels 4 Degassing technology Gas permeation through a fluoropolymer membrane Maximum flow rate...

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Page 35: ...t of an Accela LC system follow the Accela Pump Installation Checklist on page 12 and the procedures in this chapter For information about installing hardware options see Chapter 3 Hardware Upgrades Contents Accela Pump Installation Checklist Shipment Inspection Benchtop Setup Back Panel Connections Solvent Line Connections Powering On the Pump ...

Page 36: ... the steps that must be completed to properly install the Accela pump Unpack and inspect your instrument see Shipment Inspection on page 13 Read the Safety Information on page xxi Position the Accela pump appropriately on the benchtop see Benchtop Setup on page 13 Make the back panel connections see system installation procedures or Connecting the Pump to the Data System Computer on page 19 and Co...

Page 37: ...This provides access to the back panel connections and a free flow of cooling air Depending on the autosampler model follow these recommended benchtop layouts Layout for an Accela LC System with an Accela Autosampler Layout for an Accela LC System with an Accela Open Autosampler Layout for an Accela LC System with an Accela Autosampler Figure 3 shows the recommended layout for a stand alone Accela...

Page 38: ...ump and an additional detector is 1 5 m 5 ft Figure 3 Recommended layout for an LC system with an Accela Autosampler Power Communication Run Degas ACCELA Pump Power Communication Run Temperature ACCELA Autosampler Power Communication Run Lamps ACCELA PDA Detector 76 cm 30 in 114 cm 45 in Ethernet cables from the Accela Autosampler and the Accela detector to the Ethernet switch USB cable from the A...

Page 39: ...osampler with attached stand is 104 5 cm 41 in For Accela LC systems that include additional Accela instruments follow these guidelines If your LC system includes two LC pumps and a detector stack the pumps under the autosampler table and place the detector to the side of the table If your LC system includes two LC pumps and two detectors place the pump stack under the autosampler table and the de...

Page 40: ... READY RUN NC 5V 150mA GND 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 355 River Oaks Parkway San Jose CA U S A This device complies with FCC Rules Part 15 Operation is subject to the following conditions 1 This device may not cause harmful interference and 2 This device must accept any interference received including interference that may cause undesired operation This product is manufactured under a...

Page 41: ...device complies with FCC Rules Part 15 Operation is subject to the following conditions 1 This device may not cause harmful interference and 2 This device must accept any interference received including interference that may cause undesired operation This product is manufactured under and covered by one or more of the following U S patents CAUTION REFER ALL SERVICE TO QUALIFIED PERSONNEL ONLY DISC...

Page 42: ... Thermo Fisher Scientific field service engineer provides the appropriate power plugs The NEMA 5 15P plug and its corresponding receptacle are shown in Figure 6 Figure 6 NEMA 5 15P power plug and receptacle To connect the pump to line power 1 Connect the power cord to the line power receptacle on the back panel of the Accela pump see Figure 5 on page 17 2 Connect the power cord to an appropriate l...

Page 43: ...nect Cables An Accela LC system typically includes an Accela Autosampler or an Accela Open Autosampler one or two Accela pumps and one to three LC detectors The contact closure connections depend on the autosampler model For information about making the contact closure connections for your system see one of these topics Contact Closure Connections for Accela Systems with an Accela Autosampler Cont...

Page 44: ...of the pump connectors with a yellow PUMP sticker into the 8 pin socket on the back panel of the Accela pump all models Table 10 lists the pin out descriptions for the 8 pin socket 2 Plug the autosampler connector with the A S tag on its adjacent cable into the left 8 pin socket on the back panel of the Accela Autosampler 3 If your LC system contains an Accela PDA Detector an Accela UV Vis Detecto...

Page 45: ... CHASSIS GND RECORDER RECORDER 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 RESERVED RESERVED GND GND INJECT HOLD PUMP READY GRAD START 5V USB RS232 DO NOT USE TOGETHER WITH D SUB25 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 Accela PDA Detector or Accela UV Vis Detector Accela Autosampler Connector labeled DETECTOR System interconnect cable Connector ...

Page 46: ...igure 10 Accela Open Autosampler interconnect cable P N 60157 63024 Figure 11 shows the adapter cable for an Accela LC system with an Accela Open Autosampler and an Accela detector refractive index UV Vis or photodiode array detector or TSQ Series or Exactive mass spectrometer Tip An unlabeled cable equivalent to the Accela Open Autosampler interconnect cable P N 60157 63024 ships with the autosam...

Page 47: ...57 63024 on the adapter cable to the 2 pin connector labeled TO MSQ LTQ LXQ on the Accela Open Autosampler interconnect cable 3 Connect the adapter cable to pins 1 through 8 on the back panel of the Accela detector Figure 12 shows the contact closure connections for an Accela LC system with an Accela Open Autosampler Accela 600 or 1250 Pump and Accela detector Tip For a dual pump system connect th...

Page 48: ...ND ZERO READY RUN NC 5V 150mA GND NC CHASSIS GND INTEGRATOR INTEGRATOR NC CHASSIS GND RECORDER RECORDER 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 D SUB25 INTERFACE 1 TO 60157 63024 60157 63026 TO LCQ FLEET TO LTQ LXQ MSQ TO TSQ EXACTIVE TO PDA TO MSQ LTQ LXQ TO PUMP 60157 63024 TO A S Accela Open Autosampler PDA detector or UV Vis detector RI Detector Accela 600 or 1250 Pump Connection between the Ac...

Page 49: ...sub 25 pin connector IMPORTANT Use either the D sub 25 pin connector or pins 1 through 8 to connect to an external device Never use both of these connectors at the same time Table 11 D sub 25 pin connector pin out descriptions Pin Description Pin Description Pin Description 1 Rel 1 Input 10 Dig In2 19 Rel 4 NC 2 Rel 1 NC 11 Rel Error In 20 GND 3 Rel 2 NO 12 Rel Error NC 21 GND 4 Rel 3 Input 13 GND...

Page 50: ...servoir bottles and four solvent reservoir bottle caps Each solvent reservoir line consists of a 1 8 m 6 ft length of 0 0625 in ID 1 8 in OD fluorinated ethylene propylene FEP tubing with a color coded Super Flangeless fitting at one end and a 20 μm particle size stainless steel filter at the other end To install the solvent lines follow these procedures Assembling the Solvent Reservoir Bottles Co...

Page 51: ...les reside in the solvent platform at the top of the system For an LC system with an Accela Open Autosampler the solvent reservoir bottles reside in the solvent tray attached to the autosampler table IMPORTANT Use only stainless steel 20 μm particle size sinker frits to terminate the solvent reservoir lines Tip The cap is a two piece assembly The upper section snaps onto a threaded section You can...

Page 52: ...ng see Figure 14 Figure 14 Super Flangeless fitting components with color coded nuts The compression ring has two sides a flat side and an angled side see Figure 15 The flat side of the ring faces the nut and the angled side of the ring faces the narrow end of the ferrule CAUTION Use appropriate solvents with your Accela LC system Use HPLC or LCMS grade solvents that are free of particulate matter...

Page 53: ...e nut fingertight Be careful not to cross thread the fitting which can cause solvent leakage For inlet A use the blue fitting For inlet B use the yellow fitting For inlet C use the green fitting For inlet D use the orange fitting Figure 16 Vacuum degasser connections 2 To complete the installation route the solvent line through the cutouts along the left side of each Accela module Tip The Super Fl...

Page 54: ...cting the High Pressure Solvent Line to the Autosampler CAUTION To avoid damaging the degassing unit and the piston seals prime the degassing membranes by using a syringe to pull solvent through the solvent lines into the degassing unit Do not push the solvent into the degasser Pushing the solvent can generate excessive pressure and rupture the degassing membranes The maximum recommended pressure ...

Page 55: ...e the 0 6 m 2 ft length of precut tubing in the Accela Open Autosampler Accessory Kit To connect the high pressure solvent line to the LC pump outlet 1 Turn off the pump flow 2 Using the supplied two piece stainless steel fitting connect the precut stainless steel tubing to the pump outlet dynamic mixer outlet see Figure 17 or placeholder blind module outlet Figure 17 Dynamic mixer outlet Tip To i...

Page 56: ...the High Pressure Solvent Line to the Autosampler To connect the high pressure solvent line follow the procedure for your autosampler Connecting the Solvent Line to the Accela Autosampler Heat Exchanger Connecting the Solvent Line to the Accela Open Autosampler Injection Valve Connecting the Solvent Line to the Accela Autosampler Heat Exchanger The metal plate in front of the Accela Autosampler he...

Page 57: ...pler drip tray 3 Connect the tubing to the heat exchanger inlet as follows a Using a two piece stainless steel fitting connect the tubing to the free end of the union connected to the heat exchanger inlet b Using a 1 4 in open ended wrench tighten the nut Figure 20 shows the high pressure connection between the Accela Autosampler and the Accela 600 or 1250 Pump CAUTION To prevent personal injury b...

Page 58: ...m 2 ft length of precut stainless steel tubing in the Accela Open Autosampler Accessory Kit To connect the high pressure solvent line to the injection valve 1 Route the stainless steel tubing connected to the LC pump outlet through the cutout on the top of the pump enclosure behind the right door through the access slot in the detector drip tray and through the cutout on the top of the detector en...

Page 59: ...mp to the line power source The pump s power entry module is on its back panel see Figure 5 on page 17 3 Using the supplied USB cable connect the pump to the data system computer see Figure 7 on page 19 4 Connect the low and high pressure solvent lines see Solvent Line Connections on page 26 5 Press the power On switch see Figure 1 on page 2 The Power LED turns solid green To control the pump from...

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Page 61: ...e optional dynamic mixer seal wash pump or leak sensor follow the instructions in this chapter Note Because installing these hardware options involves the removal of the pump chassis from the pump enclosure Thermo Fisher Scientific recommends that only a Thermo Fisher Scientific field service engineer installs them Contents Dynamic Mixer Installation Seal Wash Pump Installation Leak Sensor Install...

Page 62: ...ls To install the dynamic mixer follow these steps 1 Removing the Pump Chassis from the Enclosure 2 Connecting the Dynamic Mixer to the Pump s Front Panel 3 Connecting the Dynamic Mixer to the Controller Board 4 Reconnecting the Pump Chassis to the Enclosure 5 Reconnecting the Static Mixer and the High Pressure Lines Tool Use 1 4 in open end wrench To disconnect the high pressure lines 2 Phillips ...

Page 63: ...from the blind outlet placeholder module see Figure 23 and Figure 24 on page 40 Figure 23 Static mixer connection to the blind outlet module 3 If the high pressure tubing is connected between the pump and the autosampler using a 1 4 in open end wrench disconnect the high pressure solvent line from the blind outlet module CAUTION To prevent electrical shock turn off the pump and disconnect it from ...

Page 64: ...Using a 2 Phillips head screwdriver remove the screw that secures the metal plate covering the LED board and then remove the metal plate see Figure 25 and Figure 26 Figure 25 Front view of the pump with the doors open 5 Disconnect the LED cable from the left door of the pump see Figure 26 Static mixer Screw that secures the metal plate Four screws that secure the enclosure to the chassis ...

Page 65: ...ic mixer go to the next procedure Connecting the Dynamic Mixer to the Pump s Front Panel Tip The seal wash pump and leak sensor installation procedures also require the removal of the pump chassis from the enclosure However removing the chassis from the enclosure is not the first step in these procedures If you are installing one of these other upgrade options do the following To continue installi...

Page 66: ...2 5 mm hex ball driver remove the two screws with washers that secure the blind outlet placeholder module to the front panel of the pump see Figure 27 Set the screws and washers aside You use them in step 5 on page 45 to secure the dynamic mixer to the pump Figure 27 Back side of the pump s front panel showing the screws with washers being removed Screws that secure the blind outlet placeholder mo...

Page 67: ...ceholder module from the front panel of the pump see Figure 28 Figure 28 Blind outlet placeholder module removed from the front panel 3 Using a 1 4 in open end wrench disconnect the static mixer from the LDA see Figure 29 and Figure 30 Figure 29 Static mixer connection to the LDA Blind outlet module Connection between LDA and static mixer ...

Page 68: ...Manual Thermo Scientific Figure 30 Static mixer disconnected from the pump 4 Insert the dynamic mixer into the port vacated by the blind outlet placeholder module see Figure 31 Figure 31 Dynamic mixer inserted into the port vacated by the blind outlet module Static mixer Dynamic mixer ...

Page 69: ...ed in step 1 on page 42 Figure 32 Dynamic mixer viewed from the inside of the pump chassis 6 Using a 2 5 mm hex ball driver tighten the hex socket screws that secure the dynamic mixer to the pump see Figure 33 Figure 33 Dynamic mixer secured to the pump chassis Go to the next procedure Connecting the Dynamic Mixer to the Controller Board Back side of the dynamic mixer Screws that secure the dynami...

Page 70: ...sis to the Enclosure Reconnecting the Pump Chassis to the Enclosure To reconnect the pump chassis to the enclosure 1 Push the pump chassis back into the enclosure and then secure the chassis to the enclosure with the four screws that you removed in step 6 on page 41 2 Reconnect the LED cable to the LED board 3 Align the hole in the LED cover plate with the port in the door for the screw Slide the ...

Page 71: ...mixer as follows a Using a 1 4 in open end wrench reconnect the static mixer to the LDA b Using a 1 4 in open end wrench connect the static mixer to the inlet of the dynamic mixer see Figure 35 Figure 35 Static mixer connected to the LDA and the dynamic mixer 2 Connect the outlet of the dynamic mixer to the autosampler inlet see High Pressure Solvent Line Between the Pump and the Autosampler on pa...

Page 72: ...s front panel as follows a Using a 2 5 mm hex ball driver remove the two hex socket screws that secure the blind cover to the front panel of the pump see Figure 36 Set the screws aside Tool Use 2 Phillips head screwdriver To disconnect the LED cover plate from the inside of the pump s left door and the chassis from the enclosure 2 5 mm hex ball driver To disconnect the screws that secure the cover...

Page 73: ... Manual 49 Figure 36 Blind cover and screws removed from the pump s front panel b With the cable connector facing up see Figure 37 slide the seal wash pump into the front panel of the pump see Figure 38 on page 50 Figure 37 Seal wash pump Blind cover Screws that secure the blind cover Cable connector ...

Page 74: ... 1250 Pump Hardware Manual Thermo Scientific Figure 38 Seal wash pump placed into the front panel of the pump c Using the two hex socket screws that you removed in step a on page 48 secure the seal wash pump to the front panel of the pump see Figure 39 Seal wash pump ...

Page 75: ...wash pump secured to the front panel of the pump d Using a 2 5 mm hex ball driver tighten the hex socket screws 3 Remove the pump chassis from the enclosure see Removing the Pump Chassis from the Enclosure on page 39 Screws that secure the seal wash pump CAUTION Observe precautions for handling electrostatic sensitive devices ...

Page 76: ...to Valve E on the Controller board see Figure 40 Figure 40 Seal wash pump connection to the Controller board 5 Reconnect the pump chassis to the enclosure see Reconnecting the Pump Chassis to the Enclosure on page 46 To set up the seal wash pump for continuous flushing go to Setting Up Continuous Flushing on page 61 Cable connection to seal wash pump Cable connection to Controller board ...

Page 77: ...ng bracket Locating fixture Two component adhesive To install the optional leak sensor 1 Turn off the power to the pump and unplug the pump from line power 2 Glue the mounting bracket for the leak sensor to the pump s drip tray as follows a Place the locating fixture against the front panel of the pump as shown in Figure 41 Figure 41 Appropriate position for the locating fixture b Mix the two comp...

Page 78: ...oir bottles Then disconnect the solvent lines from the degasser inlet ports and drain the solvent in the solvent lines into a waste container 4 Remove the LDA from the pump see Removing the LDA from the Pump on page 79 5 Remove the pump chassis from the enclosure see Removing the Pump Chassis from the Enclosure on page 39 Mounting bracket CAUTION Because the Accela pump contains a high voltage sup...

Page 79: ...onnected to the J14 connector on the Interface board and the P18 connector on the Controller board Figure 43 In step 7 on page 56 you replace this cable with a cable that connects to the J14 P18 and P8 connectors Figure 43 Pump chassis with a view of the Interface board and the Controller board J14 connector P18 connector P8 connector ...

Page 80: ...are Manual Thermo Scientific 7 Connect the new cable see Figure 44 to the J14 connector on the Interface board and the P18 and P8 connectors on the Controller board Figure 44 New cable provided in the Leak Sensor Kit J14 connector P8 connector P18 connector Leak sensor connector ...

Page 81: ...nsor onto the mounting bracket see Figure 45 Figure 45 Sliding the leak sensor onto the mounting bracket 9 Press the leak sensor latch against the mounting bracket until it clicks in place see Figure 46 Figure 46 Leak sensor secured to the mounting bracket Mounting bracket Latch Cable Leak sensor Leak sensor Leak sensor latch ...

Page 82: ...d 1250 Pump Hardware Manual Thermo Scientific 10 Route the leak sensor cable through the slot below the mounting fixture for the LDA on the front panel of the pump see Figure 47 Figure 47 Front panel of the pump with the LDA removed Slot for the leak sensor cable ...

Page 83: ...igure 48 Connection between the leak sensor cable and the new P18 to J14 cable 12 Reconnect the pump chassis to the enclosure see Reconnecting the Pump Chassis to the Enclosure on page 46 13 Reattach the LDA to the pump s front panel see Reattaching the LDA to the Pump on page 92 Connection between the leak sensor cable and the P18 to J14 cable Leak sensor cable New P18 to J14 cable ...

Page 84: ...is dry the leak sensor s LED remains green see Figure 49 If solvent comes in contact with the sensor the leak sensor s LED turns red see Figure 50 Figure 49 Green LED indicates a dry drip tray Figure 50 Red LED indicates a wet drip tray For information on specifying the configuration settings for the leak sensor see Chapter 4 Instrument Driver Configuration Green leak sensor LED Red leak sensor LE...

Page 85: ... concentration of salts The Seal Wash Pump Kit see Seal Wash Pump Kit on page 134 contains the seal wash pump the tubing and fittings that you must have to set up the seal wash pump and the cable that connects the seal wash pump to the Controller board To set up continuous flushing 1 Connect a reservoir of distilled water to the In port of the seal wash pump 2 Remove the inner wash line from the g...

Page 86: ... connect the lower guide bushing to the Out port of the seal wash pump 4 Using the tubing without fittings provided in the Seal Wash Pump Kit connect the lower wash tubing of the upper guide bushing to a waste reservoir Figure 52 shows the solvent line connections for the seal wash pump Figure 52 Seal wash pump solvent line connections Seal wash pump inlet Seal wash pump outlet Connection to waste...

Page 87: ...terprise or add the pump to an existing Accela instrument An instrument can include one or two Accela pumps To add a new Accela instrument to the ChromQuest Enterprise 1 Open the ChromQuest data system The Main Menu window appears see Figure 53 Note To configure the pump for data system control the pump must be turned on and connected to the data system computer Contents ChromQuest Instrument Conf...

Page 88: ...icon appears in the right pane Figure 53 Shortcut menu for The Enterprise in the Main Menu window 3 To open the Instrument Configuration dialog box do one of the following Select the New Instrument icon and then choose File Configure Instrument or Right click the New Instrument icon and then choose Configure Instrument from the shortcut menu The Instrument Configuration dialog box appears see Figu...

Page 89: ...distinguish it from other instruments with different configurations or stack IDs 5 In the Instrument Type list select Accela The message shown in Figure 55 appears Figure 55 Message that appears when you select an instrument type 6 Do one of the following To create a new instrument click OK to close the message box or To modify the configuration of an existing instrument click Cancel and do not ma...

Page 90: ...in the previous procedure 3 In the Available Modules list double click Accela Pump A copy of the Accela Pump icon appears in the Configured Modules pane 4 In the Configured Modules pane double click Accela Pump The Accela Pump Configuration dialog box appears 5 In the Serial Number list select the serial number for the pump If you have connected two pumps to the data system computer the last five ...

Page 91: ...readback changes from Disconnected to Connected and the Pump Type readback for Pump 1 and Pump 2 for a dual pump system specifies Accela 600 or Accela 1250 see Figure 58 In a dual pump system the two pumps can be different pump types Figure 58 Accela Pump Configuration dialog box with the pump s serial number selected 355 River Oaks Parkway San Jose CA U S A Model 1250 Pump Serial No 925000 ...

Page 92: ...ee Figure 59 Figure 59 Leak Sensor Configuration dialog box c Select the action that you want the pump to take if the leak sensor detects a leak d Click OK to accept the setting and close the Leak Sensor Configuration dialog box 7 In the Default Pressure Units list select the pressure units that you want the data system to display The available selections are PSI bar and MPa 8 Click OK to accept t...

Page 93: ...nt configuration To add one or two Accela pumps to the Xcalibur instrument configuration 1 If the Xcalibur data system is open close it 2 Connect the pump or pumps to the data system computer 3 Turn on pump 1 by pressing its power switch If you have a dual pump system turn off pump 2 4 For Xcalibur versions earlier than 2 1 from the computer desktop choose Start All Programs Xcalibur Instrument Co...

Page 94: ...ent Configuration To add two pumps to the instrument configuration go to step 6 of this procedure 6 To add two pumps to the instrument configuration do the following a Follow step 5 through step 10 on pages 72 to 73 in Adding Pump 2 to the Instrument Configuration b Ensure that pump 2 is connected to the data system computer c Turn on pump 2 d Reopen the Instrument Configuration window as describe...

Page 95: ... 3 Close the data system if it is open Tip To increase throughput for a gradient analysis you can set up your liquid chromatography mass spectrometry LC MS system in a dual pump dual column configuration and equilibrate one column while performing sample elution with the other column To alternate columns between consecutive injections use two instrument methods In the first method pump 1 produces ...

Page 96: ... Unloading Device Drivers status box Figure 62 Thermo Foundation Instrument Configuration dialog box 5 In the Available Devices area double click the Accela 600 or the Accela 1250 icon A copy of the Accela pump type icon appears in the Configured Devices area Note Because you can install only one Accela pump driver the Available Devices area contains either an Accela 600 or Accela 1250 icon Tip Al...

Page 97: ... pump type dialog box 8 If your Accela pump has a leak sensor select the Leak Sensor Installed check box The leak sensor is an option for the Accela 600 Pump and the Accela 1250 Pump The leak sensor has an optical sensor that turns red when covered by liquid For instructions about installing the leak sensor see Leak Sensor Installation on page 53 For information about setting up leak sensor action...

Page 98: ...ge 72 followed by the Thermo Foundation Instrument Configuration application 4 In the Configured Devices area double click the Accela pump type icon The Accela pump type dialog box appears 5 Select the Pump 2 check box see Figure 64 Figure 64 Accela 1250 Pump dialog box with the settings for a dual pump system 6 Ensure that the Serial Number list displays the pump s serial number and that the Stat...

Page 99: ...r s optical LED turns red and the leak sensor sends a signal to the data system To set up the leak sensor actions 1 Check the configuration settings for your Accela pump see Foundation Instrument Configuration on page 71 Ensure that the Leak Sensor Installed check box is selected 2 Open the Xcalibur Instrument Setup window 3 In the view bar click the icon that represents your Accela pump The instr...

Page 100: ...ving and the sequence pauses Set Instrument to Error State Immediately When the leak sensor detects the presence of liquid on the pump s drip tray the pump pistons stop moving and the sequence pauses Tip To restart the pump after the leak sensor detects a leak do the following 1 Stop the sequence and delete it from the run queue 2 Close the Xcalibur data system 3 Fix the leak and make sure that th...

Page 101: ...off the power and disconnect the power cable prior to servicing the instrument There is no need to open the pump enclosure however as all user serviceable components are outside of the instrument Contents Maintenance Schedule Accessing Disassembling and Reassembling the LDA Replacing the Primary Piston Seals Replacing the Secondary Piston Seals Cleaning the Check Valves Cleaning the Pistons Cleani...

Page 102: ... mobile phase pump a non buffered solvent that is miscible with the buffered mobile phase through the system before pumping methanol or IPA through the system Table 12 Maintenance frequency Procedure Frequency Page Flushing the LDA If the LC pump does not have the optional seal wash pump flush the LDA once per day if you use buffered mobile phases and once per month if you use mobile phases that c...

Page 103: ... 4 Reattaching the LDA to the Pump For a description of the LDA see Liquid Displacement Assembly LDA on page 5 Removing the LDA from the Pump Remove the LDA to access the piston seals which you replace during regularly scheduled preventive maintenance Removing the LDA requires the following tools CAUTION When working with the wetted components of the Accela pump wear gloves Tool Use 1 4 in open en...

Page 104: ... Turn off the power to the pump 2 Unplug all four inlet proportioning valves from the front panel see Figure 66 and Figure 67 Figure 66 Four proportioning valves plugged into the front panel Figure 67 Proportioning valve plugs unplugged from the front panel Four plugs for the proportioning valves Four solvent lines connected to the inlet module ...

Page 105: ...ottles above the solvent level 4 Unscrew all four solvent lines from the inlet module see Figure 66 on page 80 5 Using a 1 4 in open end wrench unscrew the static mixer at the outlet of the LDA and at the inlet of the placeholder module see Figure 68 Figure 68 Static mixer connections to the LDA and the outlet module LDA outlet Static mixer Placeholder module inlet ...

Page 106: ... 5 mm hex ball driver loosen the two M3 4 0 cm screws on the Z arm cover and remove the cover see Figure 69 Figure 69 Z arm cover in place over the Z arms 7 Using a 4 mm hex wrench loosen the two clamping screws by three or four turns see Figure 70 Figure 70 Loosening the two clamping screws M3 4 0 cm screws Z arm cover Clamping screws ...

Page 107: ...dware Manual 83 8 Pull out the two piston retaining springs see Figure 71 Figure 71 Piston retaining springs 9 Gently push the piston heads to the left so that they do not contact the Z arms see Figure 72 Figure 72 Piston heads pushed to the left away from the Z arms Piston retaining springs Piston head Z arm ...

Page 108: ...DA attachment screws see Figure 73 Figure 73 Loosening the three LDA attachment screws 11 Gently pull the LDA off the two guide pins Do not let the pistons touch the Z arms see Figure 74 CAUTION To avoid breaking valve D do not touch or apply force to the valve when loosening the LDA attachment screw LDA attachment screws Valve D in front of the lower right attachment screw ...

Page 109: ...e Figure 75 shows the proportioning valve and cable assembly The cable is hardwired to the valve As you disassemble the LDA take care to avoid damaging the electrical wires inside the insulated cable Guide pins Pistons CAUTION The proportioning valve cables are hardwired to the proportioning valves Attempting to remove the cables from the valves can break the electrical wires inside the insulated ...

Page 110: ...al Thermo Scientific Figure 75 Proportioning valve and cable assembly To disassemble the LDA 1 Remove the piston back flush tubes by gently pulling them off see Figure 76 Figure 76 Location of the piston back flush tubes Proportioning valve Insulated cable with two electrical wires Piston back flush tubes ...

Page 111: ...rews that secure the holding hooks see Figure 77 Figure 77 Location of the fastening screws that secure the holding hooks 3 Carefully remove the pistons guide bushings and holding hooks from the LDA body see Figure 78 Figure 78 LDA with the pistons guide bushings and holding hooks removed Fastening screws Note Contrary to this drawing you cannot remove the proportioning valve cables from the propo...

Page 112: ...screw Remove the compression lid by sliding it out see Figure 79 Figure 79 LDA with and without the compression lid 5 Slide out the outlet module check valves and displacement chambers see Figure 80 Figure 80 Sliding out the check valves displacement chambers and outlet module Compression lid removed Check valve Check valve Lower displacement h b Upper displacement h b Outlet module ...

Page 113: ...ate orientation see Figure 81 and then insert it into the main body of the LDA see Figure 82 Figure 81 Check valve orientation Figure 82 Inserting the displacement chambers check valves and outlet module into the LDA CAUTION The proportioning valve cables are hardwired to the proportioning valves Attempting to remove the cables from the valves can break the electrical wires inside the insulated ca...

Page 114: ...rt it into the main body of the LDA 5 Insert the upper displacement chamber into the main body of the LDA 6 Insert the outlet module into the main body of the LDA 7 Carefully slide the compression lid into the LDA main body see Figure 83 If the compression lid does not fit verify that the parts are installed correctly Figure 83 Installing the compression lid 8 Using the 4 mm hex wrench firmly tigh...

Page 115: ...so that they protrude away from the holding hook see Figure 103 on page 102 10 Carefully insert the piston into the bore of the displacement chamber and seal see Figure 85 At the same time tighten the holding hook using the 4 mm hex ball driver Repeat the same procedure for the second piston unit Figure 85 Inserting the piston guide bushing holding hook assembly 11 Firmly tighten both holding hook...

Page 116: ... maintenance procedure that required you to remove it To reattach the LDA to the pump 1 Align the pistons so that they do not touch the Z arms while you insert them Do not insert the piston springs at this time 2 Reinstall the piston back flush tubes see Figure 87 Figure 87 LDA with the piston back flush tubes reinstalled 3 Place the LDA onto the drive unit using the two alignment pins as a guide ...

Page 117: ...the LDA onto the pump 4 Using a 4 mm hex ball driver tighten the three LDA attachment screws see Figure 89 Figure 89 LDA attachment screws Alignment pins CAUTION When tightening the screw near the inlet valve do not touch or apply force on inlet valve D or it might break LDA attachment screws Valve D in front of the lower right attachment screw ...

Page 118: ...cientific 5 Insert the piston springs The lip of the spring faces toward the right see Figure 90 Figure 90 Proper orientation of the piston retaining springs 6 Using the 4 mm hex wrench tighten the two clamping screws see Figure 91 Figure 91 Tightening the two clamping screws Piston retaining springs Clamping screws ...

Page 119: ... tighten the screws using a 2 5 mm hex ball driver see Figure 92 Figure 92 Z arm cover in place 8 Carefully reconnect the static mixer see Figure 93 to the LDA outlet and the placeholder module inlet Figure 93 Static mixer connected to the LDA and the placeholder module M3 4 0 cm length screws Z arm cover LDA outlet Static mixer Placeholder module inlet ...

Page 120: ...00 and 1250 Pump Hardware Manual Thermo Scientific 9 Reconnect the solvent lines to the inlet module 10 Plug the proportioning valve cables into the receptacles on the front panel of the pump see Figure 94 Figure 94 Plugging the proportioning valve cables into the receptacles ...

Page 121: ...nths depending on your pump usage The primary piston seals for the Accela pumps are made of GFP and have a spring made of Hastelloy an alloy of stainless steel see Figure 95 Figure 95 Primary piston seal with a view of the spring To replace the primary piston seals 1 Remove the LDA from the pump see Removing the LDA from the Pump on page 79 2 Disassemble the LDA see Disassembling the LDA on page 8...

Page 122: ...the primary piston seal 7 Check the piston for contamination and scratches and then do the following If the piston is in good condition go to the next step If the piston is scratched replace it If the piston needs cleaning clean it as described in Cleaning the Pistons on page 103 8 Insert the piston into the guide bushing 9 With the spring see Figure 95 on page 97 facing away from the guide bushin...

Page 123: ... into the bore of the displacement chamber Do not apply any side load onto the seal during this process see Figure 99 Figure 99 Using the guide bushing and piston to insert the piston seal 11 Hold down the guide bushing and slide the piston up until it is out of the seal see Figure 100 Figure 100 Sliding the piston out of the seal ...

Page 124: ...splacement chamber see Figure 101 Figure 101 Installed piston seal 13 Repeat step 3 through step 12 to replace the primary piston seal of the second displacement chamber with a new piston seal 14 Reassemble the LDA and reattach it to the pump see Reassembling the LDA on page 89 15 Reattach the LDA to the pump see Reattaching the LDA to the Pump on page 92 Piston seal ...

Page 125: ... on page 85 3 Remove the used secondary piston seal as follows a Carefully pull the piston out of the holding hook b Dispose of the used secondary piston seal 4 Check the piston for contamination and scratches and then do the following If the piston is in good condition go to the next step If the piston is scratched or appears cloudy replace it If the piston needs cleaning clean it as described in...

Page 126: ...re 103 Proper alignment of the piston wash tubes 8 Gently push the new secondary piston seal into its groove by pushing the guide bushing up toward the end of the holding hook see Figure 104 Figure 104 Holding hook piston guide bushing assembly 9 Repeat step 5 through step 8 for the remaining secondary piston seal 10 Reassemble the LDA see Reassembling the LDA on page 89 11 Reattach the LDA to the...

Page 127: ...anol 5 Verify that fluid flows freely through the check valve If you cannot achieve free flow through the check valve replace it with a new one 6 Reassemble the LDA see Reassembling the LDA on page 89 7 Reattach the LDA to the pump see Reattaching the LDA to the Pump on page 92 Cleaning the Pistons Clean the pistons as part of a regularly scheduled preventive maintenance procedure or if you observ...

Page 128: ...rade methanol or IPA This removes the aliphatic amine residue that can build up on the sapphire check valve seats and cause the check valves to stick To flush a buffered aqueous mobile phase out of the LDA 1 Set up the system to pump LC MS grade deionized water through the LDA for 10 minutes 2 Pump LC MS grade methanol or IPA through the LDA for 10 minutes 3 Pump LC MS grade deionized water throug...

Page 129: ...by this precipitate can scratch the piston and shorten the life of the piston seals If you use mobile phases that contain a high concentration of salts and you do not have the seal wash pump flush the piston seals at least once a day The seal wash pump is an optional pump that periodically flushes the piston guide bushings see Seal Wash Pump Installation on page 48 To flush the piston guide bushin...

Page 130: ...e valve remove the syringe and return to the initial conditions 5 Check the pressure variation to determine if removing the trapped air stabilized the system pressure Note All low pressure mixing HPLC pumps require eluents that have been properly degassed The pump has a built in vacuum degasser Plumbing the pump to bypass this degasser introduces a potentially unacceptable level of air into the pi...

Page 131: ...emove the film seals as this might cause leaking 5 Place the displacement chambers and the inlet module in a container of water and place the container in an ultrasonic bath for 10 minutes 6 Place the displacement chamber and the inlet module in a container of acetone and place the container in an ultrasonic bath for 10 minutes 7 Install the PEEK film seals if they are not damaged otherwise replac...

Page 132: ...igure 107 to the right and pull out the fuse box Figure 107 Power entry module and fuse label 3 Remove the fuses and replace with the appropriate fuses 4 Plug the pump power cord into the power outlet CAUTION To avoid an electrical shock turn off the power to the pump and unplug the pump from line power before you remove the fuse holder Line power Fuse 100 Vac or 115 Vac 5 20 mm 2 0 A 250 V time l...

Page 133: ...tests and troubleshooting guides in this chapter Diagnostic Tests Run these tests if you want to validate the pump s performance or if you suspect the pump is not functioning properly Testing the Flow Rate Testing the Residual Pulsation Testing the Pump Proportioning Accuracy Contents Diagnostic Tests Error Messages Troubleshooting Guides ...

Page 134: ...to inlet channel A 3 Use the instrument control system to set channel A to 100 4 Set the flow rate to 1 mL min 5 Do one of the following Connect the other end of the restrictor cartridge to an instrument that measures flow rate or Connect the other end of the restrictor cartridge to a length of tubing and place the tubing into an empty 10 mL graduated cylinder 6 Start the pump wait 2 minutes and t...

Page 135: ...the pressure is 0 6 Set channel A to 100 7 Set the flow rate to 500 μL min 8 Start the pump wait 1 minute and then close the purge valve 9 Wait 5 minutes to allow the pump pressure to equilibrate 10 Observe the pressure graph for pressure fluctuations The pressure standard deviation should be less than 1 bar 14 5 psi Table 13 Reasons for unstable flow rates Possible reason for unstable flow rate C...

Page 136: ...w cell see Figure 109 Figure 109 LDA attached to a flow restrictor d Fill a mobile phase reservoir bottle with HPLC grade water and connect it to inlet channels A and C e Fill a mobile phase reservoir bottle with HPLC grade water spiked with 0 5 acetone and connect it to inlet channels B and D 2 Draw solvent through all four channels a Set each channel to 25 b Open the purge valve c Set the flow r...

Page 137: ...B Spiked water C Water D Spiked water Prequilibration 0 100 0 0 0 Step up 0 0 1 0 1 25 25 0 0 Hold 0 1 5 0 5 0 25 25 0 0 Step down 5 0 5 1 5 1 100 0 0 0 Hold 5 1 9 0 9 0 100 0 0 0 Step A 9 0 9 1 9 1 75 25 0 0 Hold 9 1 13 5 13 5 75 25 0 0 Step B 13 5 13 6 13 6 50 50 0 0 Hold 13 6 18 0 18 0 50 50 0 0 Step C 18 0 18 1 18 1 25 75 0 0 Hold 18 1 22 5 22 5 25 75 0 0 Step D 22 5 22 6 22 6 0 100 0 0 Hold 2...

Page 138: ...m baseline to top see Figure 110 Figure 110 Step gradient profile for testing compositional accuracy Accela pump validation software Hold 48 6 53 0 53 0 0 0 0 100 Step down 53 0 53 1 53 1 0 0 100 0 Hold 53 1 60 0 60 0 0 0 100 0 Table 14 Gradient file for quaternary pumps Sheet 2 of 2 Gradient Time minutes A Water B Spiked water C Water D Spiked water A B C D E F G H ...

Page 139: ...criteria in Table 16 9 If any value falls outside the range call Thermo Fisher Scientific Technical Support Table 15 Step gradient calculation Step Calculation 25 50 75 Table 16 Pass criteria for gradient test Gradient forming performance Pass criteria 25 Step Height _______ 24 5 25 5 50 Step Height _______ 49 5 50 5 75 Step Height _______ 74 5 75 5 A E 2 D H 2 100 B F 2 D H 2 100 C G 2 D H 2 100 ...

Page 140: ...ror The system expected an index signal that never arrived Physical problem with the hardware or connectors Contact an authorized dealer Leak detected Indicates the presence of liquid on the pump s drip tray Motor Error The motor is malfunctioning Indicates that too much torque is required to drive the pump accurately Position Error The system was unable to maintain accurate positioning of the mot...

Page 141: ...he cable Defective communication cable Replace the cable Wrong or corrupted firmware Download the current firmware to the pump Flow is unstable or pulsating Check valve failure Clean or replace the check valve See Cleaning the Check Valves on page 103 Immiscible solvents Test or change the solvent system Insufficient degassing Use helium degassing Solvent frit clogged Replace the solvent frit Insu...

Page 142: ...on Vacuum pump is not running Possible sensor or control board fault Contact your Thermo Fisher Scientific field service representative Power and Vacuum LEDs illuminate green but the pump cannot be heard Due to the design of the pump and degasser the pump is very silent at low RPMs even though vacuum is good and degassing is normal Monitor the UV absorbance of non degassed methanol at 215 nm versu...

Page 143: ...ermo Fisher Scientific customer service representative or technical support Contents Main Body Accela 600 Pump LDA Accela 1250 Pump LDA Inlet Valve Assembly Service Spares Accela 600 Pump Accessory Kit Accela 1250 Pump Accessory Kit Accela System Kit Accela 600 Pump and Accela 1250 Pump Inlet Tubing Kit Compression Fittings for High Pressure Connections Tools Seal Wash Pump Kit Dynamic Mixer Leak ...

Page 144: ...0297 2 Screw ISO 4762 M3 8a a The length of the screw is 8 mm and the diameter is 3 mm N A 3 Cover metal plate Accela 600 and 1250 N A 4 LDA Accela 600 00950 01 00294 5 Mixer static Accela 600 and 1250 00950 01 00292 6 Placeholder Accela 600 and 1250 00950 01 00293 7 Piston spring stainless steel 00201 11324 8 Z arm cover Accela 600 N A 9 Screw M3 4 0b b The length of the screw is 4 cm and the dia...

Page 145: ...gure 111 Main pump front view 1 Degassing bracket 2 Screw ISO 4762 M3 8 3 Blind cover 4 LDA 5 Mixer static 6 Placeholder blind outlet module 7 Piston spring 8 Z arm cover 9 Screw M3 40 10 Front panel 11 Drip tray 12 Cable valve D 16 Cable valve A 14 Cable valve C 13 Inlet Module 15 Cable valve B ...

Page 146: ...tem Quantity Description Part number 1 1 Outlet module complete compatible with both the Accela 600 Pump and the Accela 1250 Pump 00950 01 00284 2 5 Ultra high pressure PEEK sealing ring 00950 01 10013 3 1 Displacement chamber upper 00950 01 00282 4 2 Check valve titanium 00110 05110 5 1 Displacement chamber lower 00950 01 00281 6 1 LDA main body N A 7 2 Piston unit S finish ceramic 00201 11324 8 ...

Page 147: ...Pump LDA parts 1 Outlet module 2 UHP sealing ring PEEK 3 Upper displacement chamber 4 Check valve titanium 5 Lower displacement chamber 6 UHP LDA main body 7 Piston unit S finish ceramic 8 Holding hook 9 Secondary piston seal PE 10 Guide bushing 11 Primary piston seal GFP 12 LDA adapter 13 Compression lid including set screw ...

Page 148: ...uantity Description Part number 1 1 Outlet module complete compatible with both the Accela 600 Pump and the Accela 1250 Pump 00950 01 00284 2 5 Ultra high pressure PEEK sealing ring 00950 01 10013 3 1 Displacement chamber upper 00950 01 00123 4 2 Check valve titanium and ruby 00110 05110 5 1 Displacement chamber lower 00950 01 00121 6 1 LDA main body N A 7 2 Piston unit sapphire 00950 01 00126 8 2...

Page 149: ...a 1250 Pump LDA parts 1 Outlet module 2 UHP PEEK sealing ring 3 Upper displacement chamber 4 Check valve titanium 5 Lower displacement chamber 6 UHP LDA main body 7 Sapphire piston 8 Holding hook 9 Secondary piston seal GFP 10 Guide bushing 11 Primary piston seal GFP 12 LDA adapter 13 Compression lid including set screw ...

Page 150: ...gure 114 Figure 114 Inlet valve assembly Table 23 Inlet valve assembly parts compatible with both pumps Item Quantity Description Part number 1 4 Proportioning valve and cable assembly 00950 01 00372 2 1 Flow brake 00950 01 00287 3 1 O ring 2 69 1 14 mm 00950 01 00286 4 4 Parts for valve fixation 00950 01 00288 3 O ring 2 69 1 14 2 Flow brake 4 Parts for valve fixation 1 Proportioning valve and ca...

Page 151: ...alve Accela 600 Pump RoHS see Figure 124 on page 142 00950 01 00289 Cable assembly force sensor Accela 600 Pump RoHS 00950 01 00290 Cable assembly digital I O Accela 600 Pump RoHS 00950 01 00291 Cable Bowden Accela 00302 99 00010 Cable motor index MS pump 00950 01 00001 Cable USB 2 0 A to B 12 in UL RoHS 00302 99 00009 Fuseholder drawer 5 20 mm 2 pole RoHS 2109 0740 Fuse 1 0 A 250 V 5 20 mm time l...

Page 152: ...1 00283 Chamber displacement lower Accela 600 Pump 00950 01 00281 Chamber displacement upper Accela 600 Pump 00950 01 00282 Gearbox new Accela 600 Pump RoHS 00950 01 00295 Piston 1 8 in diameter tetragonal zirconia polycrystal TZP 00201 11324 Seal piston secondary and primary GFP 00109 03 00011 Table 26 Accela 1250 Pump service spares Description Part number Bushing guide Accela Pump 00950 01 0012...

Page 153: ... time lag UL VDE RoHS N A 1 Fuse 2 0 A time lag 5 20 mm RoHS IEC UL CSA N A 1 Wrench 1 4 5 16 in RoHS N A 1 Tubing convoluted pump waste F5034 040 1 Syringe Luer Lok 10 mL N A 1 Screwdriver ball driver hex 4 mm RoHS N A 1 Connector cable plug 8 positions 3 81 mm pitch Phoenix RoHS 00004 02511 1 Cable USB A to B 3 0 m shielded RoHS 00302 99 00014 1 Wrench L hex 4 mm RoHS N A 1 Tool hex ball driver ...

Page 154: ...UL CSA IEC N A 2 Fuse 1 0 A 250 V 5 20 mm time lag UL VDE RoHS N A 1 Wrench 1 4 5 16 in super krome RoHS N A 1 Tubing convoluted pump waste F5034 040 1 Syringe Luer Lok 10 mL N A 1 Screwdriver ball driver hex 4 mm RoHS N A 1 Connector cable plug 8 positions 3 81 mm pitch Phoenix RoHS N A 1 Cable USB A to B 3 0 m 10 ft shielded RoHS 00302 99 00014 1 Wrench L hex 4 mm RoHS N A 1 Tool ball driver 2 5...

Page 155: ...0024 1 Ethernet cable with two ferrites 97355 98006 1 System interconnect cable 7 connector cable for an Accela UHPLC system with an Accela Autosampler 60053 63034 1 UHP filter cartridge 0 5 μm particle size Teflon 00109 99 00020 1 Solvent bottle holder solvent platform for Accela system that contains the Accela Autosampler 60057 60014 4 Solvent bottle 1 liter 1413 0430 4 Solvent bottle caps outer...

Page 156: ...ess steel 2522 1880 2 Vial kit clear vials with 1 8 mL capacity black screw on caps 100 vials and caps per package A4954 010 1 Instrument control software LC Devices for use with the Xcalibur data system and several other Thermo Scientific mass spectrometry applications 60257 62005 1 Instrument control software Thermo pumps add on for the ChromQuest 5 0 data system CHROM 93034 1 Instrument control...

Page 157: ...essure solvent line between the pump and the autosampler Tools Table 31 lists the tools that you must have to perform maintenance on the pump Note An earlier version of the Accela 600 Pump Inlet Tubing Kit contained four preassembled solvent reservoir lines Each solvent line consisted of a 3 m length of 0 0625 in ID 1 8 in OD FEP tubing with a 1 4 28 PEEK flangeless fitting at one end and a 20 μm ...

Page 158: ...he seal wash pump the tubing and fittings that you must have to set up the seal wash pump and the cable that connects the seal wash pump to the Controller board Figure 115 through Figure 117 show the components of the Seal Wash Pump Kit Figure 115 Tubing fittings solvent filter and solvent bottle in the Seal Wash Pump Kit Figure 116 Seal wash pump in the Seal Wash Pump Kit ...

Page 159: ... Dynamic mixer Leak Sensor Kit The Leak Sensor Kit P N 00960 01 00024 contains the following items Leak sensor with attached cable Mounting bracket Locating fixture Two component adhesive Connect to the seal wash pump Connect to the Controller board Tip To secure the dynamic mixer to the Accela 600 Pump use the screws that secure the blind outlet placeholder module Inlet port Outlet port Connects ...

Page 160: ......

Page 161: ...e Components This appendix describes legacy hardware components for the Accela pumps Proportioning Valves Figure 119 shows the original proportioning valves for the Accela pumps Contents Proportioning Valves Fittings and Tubing Replacement Parts for the Legacy Proportioning Valves ...

Page 162: ...g valves To remove the LDA from the pump s front panel 1 Turn off the power to the pump 2 Unplug all four inlet proportioning valves see Figure 120 and Figure 121 Figure 120 Inlet valves connected to the LDA Vacuum degasser assembly Liquid displacement assembly Optional seal wash pump or cover plate Static mixer Purge valve Optional dynamic mixer or outlet adapter Inlet proportioning valves ...

Page 163: ...1250 pumps P N 60157 62008 contained four solvent reservoir lines Each solvent line consisted of a 3 m 10 ft length of 1 16 in ID 1 8 in OD FEP tubing with a 1 4 in 28 PEEK flangeless fitting at one end and a 20 μm particle size stainless steel filter at the other end To install the low pressure preassembled solvent lines 1 Assemble the solvent reservoir bottles as follows a Label the four solvent...

Page 164: ...es reside in the solvent tray attached to the autosampler table 3 For each solvent reservoir line connect the flangeless fitting to the appropriate inlet port of the built in degassing unit Figure 123 shows the flangeless fitting connected to the solvent reservoir line The flangeless fittings come in four colors blue green yellow and orange IMPORTANT Only use stainless steel 20 μm particle size si...

Page 165: ... module or the dynamic mixer Do not use the three piece fittings supplied in the legacy version of the Accela System Kit Installing the three piece fitting can cause leaks because it is too short for the port Replacement Parts for the Legacy Proportioning Valves You cannot upgrade the proportioning valves To replace a legacy proportioning valve use the following part numbers Figure 124 shows the p...

Page 166: ...or the Legacy Proportioning Valves 142 Accela 600 and 1250 Pump Hardware Manual Thermo Scientific Figure 124 Proportioning valve cable Figure 125 Inlet valve assembly 2 O ring 2 69 1 14 mm 1 Flow brake 3 Parts for valve fixation 4 Proportioning valve ...

Page 167: ...ord 18 USB 19 check valves cleaning 103 sticking 104 checklist installation 12 ChromQuest data system instrument configuration 63 cleaning check valves 103 displacement chambers 107 inlet module 107 instrument casing 104 pistons 103 compliance FCC v regulatory iii WEEE vii computer connecting 19 configuration for the ChromQuest data system 63 for the Foundation application 72 for the Xcalibur 2 0 ...

Page 168: ... seal wash pump 48 serial communication cable 19 system interconnect cable 20 unpacking 13 instrument casing cleaning 104 interconnect cable installation 20 K kits Accela 1250 Pump Accessory Kit 130 Accela 600 Pump Accessory Kit 129 Accela 600 Pump Inlet Tubing Kit 133 Accela System 131 Leak Sensor Kit 135 Seal Wash Pump Kit 134 L layout LC system 13 16 LDA assembling 89 description 5 disassemblin...

Page 169: ...ty information xxi safety standards iii seal wash pump description 6 installing 48 ordering information 134 seals pump primary replacing 97 secondary replacing 101 secondary piston seals replacing 101 serial number pump 66 solenoid error 116 solvent lines connecting the low pressure lines 26 non metallic filters 27 preassembled low pressure legacy 139 solvent requirements xxiii solvent reservoir b...

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