12. Performance Testing / Quality Control
2004-10
Technical Manual for sunrise RC, TW, TS, TC, BC, 6F No. T 137 302 Rev No. 1.1
12-3
Microplates. The best results are obtained when clear flat-bottom microplates
are used.
Depending on the type of microplate being used, the measurement result
may vary.
Take care especially when using round bottom plates or the strip
frames because it is possible that the measurement results might differ
slightly to what is described in the specifications.
Make sure that the type of microplate used with the
sunrise
Absorbance
Reader is suitable for the respective application. This applies particularly to
IVD applications.
2. Use only perfectly clean microplates.
3. Do not allow dust to settle onto the solutions or the microplate, if the
microplate is left to stand for a time before the measurement.
It is recommended to use a cover for protection.
4. Inaccuracies in the amount of solution pipetted has a greater effect on the
results obtained, when small amounts of solutions are used.
It is recommended that a minimum of 200 microliters is used in each well.
5. The form of the meniscus of the solution can cause inaccuracies in the
results, particularly if small amounts of solution are used (see 12.3.3 High
Meniscus Liquids).
Caution
Make sure the suitability of the microplate used in combination with the
amount of solution, meniscus properties and the measurement mode is
appropriate for the current application. This is especially necessary
when performing measurements for IVD applications.
