12. Performance Testing / Quality Control
2004-10
Technical Manual for sunrise RC, TW, TS, TC, BC, 6F No. T 137 302 Rev No. 1.1
12-9
12.4 Quality Control Testing
12.4.1 Instrument
Precision
This procedure can be used to check the precision of the measurements from
one microplate to another.
Fill a new microplate with a freshly prepared Methyl Orange in 0.1 % Tween 20
solution, use different dilutions of the solution in each well so that a range of
optical densities is obtained. Make sure that the wells contain at least 200
microliters.
Define a test run using the 492 nm filter and then measure the microplate at least
three times.
For each well calculate the following:
•
the average OD value
•
the highest and lowest values
•
the difference between the average, highest and lowest values
Example for a standard instrument
Readings 0.000 to 2.000 Abs.
The difference between the average and the highest and lowest values for the
same well should be /- 1.0 % and +/- 0.010 Abs.
e.g. 1.0 +/- 0.020 Abs.
Readings 2.001 to 3.000 Abs.
The difference between the average and the highest and lowest values for the
same well should be /- 1.5 % and +/- 0.010 Abs.
e.g. 2.4 +/- 0.046 Abs.
Readings above 3.000 Abs.
Readings above 3.000 Abs. are only used as an indication and the precision
cannot be guaranteed.
