SYSMEX XE-5000, Instruction Manual, Page: 67 / 262

CHAPTER 6 Operation
Sysmex XE-5000 Instructions for Use 6-13
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• Manual closed mode
In manual closed mode, the sampler is used to aspirate the
sample, without opening the cap of the sample tube. This
mode is basically the same as manual, HPC and body fluid
modes; mixing and continuous analysis can not be
performed automatically.
6.16 Preparing sample analysis
Samples
• Collect the specified amount of blood (corresponding to the
amount of EDTA anticoagulant).
• Using CELLPACK dispensed ahead of time, dilute the
sample to a 1:5 ratio for capillary mode.
• Sample volumes
Manual, capillary and manual closed mode
HPC analysis and Body fluid analysis
Sampler mode
Note:
HPC analysis and body fluid analysis can only be
performed in manual mode.
Analysis
mode
Manual Capillary Manual Closed
Required
Sample
1.0 mL or more 40
µ
L or more 1.0 mL or more
Aspirated
Sample
Approx. 130
µ
L Approx. 130
µ
L Approx. 200
µ
L
Analysis mode HPC Body fluid
Required Sample 1.0 mL or more 500
µ
L or more
Aspirated Sample Approx. 130
µ
L Approx. 130
µ
L
Diameter of Sample Tube
12 mm 15 mm
Required Sample 1.0 to 5 mL 1.0 to 7 mL
Aspirated Sample 200
µ
L