SYSMEX XE-5000, Instruction Manual, Page: 244 / 262

CHAPTER 11 Technical Information
11-26 Sysmex XE-5000 Instructions for Use
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High Fluorescence Ratio:
Immature Reticulocyte Fraction:
LFR: Low Fluorescence Ratio
MFR: Middle Fluorescence Ratio
HFR: High Fluorescence Ratio
IRF:
Immature Reticulocyte Fraction
RET-He (Reticulocyte Hemoglobin equivalent) :
The RET-He is a unique parameter developed by Sysmex that
is derived using the reticulocyte scattered light signals and a
proprietary Sysmex calculation equation.
IPF (Immature Platelet Fraction) :
10. RBC/PLT particle size distribution analysis
RBC particle size distribution
The RBC (red blood cells) are found between two
discriminators, a lower discriminator (LD) and upper
discriminator (UD), which are automatically set up between
25 – 75 fL and 200 – 250 fL, respectively.
Particle size distributions are checked for abnormalities,
including abnormal relative frequencies at the different
discriminator levels, existence of two or more peaks and
abnormal distribution widths.
The XE-5000 expresses the RBC distribution width (RDW)
according to the two methods shown below.
RDW-SD
With the peak height assumed to be 100%, the distribution
width at the 20% frequency level is RDW-SD. Units are
expressed in fL (femtoliters), with 1 fL equal to 10
-15
L.
Particle count in HFR zone
HFR =
✕
100
Particle count in reticulocyte zone
IRF = MFR + HFR
Particle in IPF classified area in RET channel
IPF=
✕
100
Platelet count in RET channel
100%
20%
RDW