SYSMEX XE-5000, Instruction Manual, Page: 238 / 262

CHAPTER 11 Technical Information
11-20 Sysmex XE-5000 Instructions for Use
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IMI analysis procedure
IMI (immature information) indicates information on immature
cells (granulocytes). The procedure for analyzing IMI is
explained as follows:
1. Blood is aspirated from the manual aspiration pipette to
the sample rotor valve.
2. 2.4 µL of blood, measured by the sample rotor valve, is
diluted with 0.5976 mL of STROMATOLYSER-IM to a ratio
of 1:250, and then sent to the IMI detector as the diluted
sample.
After reacting for about 13 seconds in this condition, the
red blood cells are hemolyzed and the cytoplasm of white
blood cells other than the immature granulocytes is
released/dissolved and are reduced in size.
3. The diluted sample is aspirated, via the aperture, and
250 µL is accurately measured by the float-type
manometer.
Detection is performed via the RF/DC detection method.
IMI analysis reagent
STROMATOLYSER-IM: 0.5976 mL
Sample rotor valve
Sample tube
(blood: 2.4
µ
L)
IMI detector