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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM411 · Revised 4/15
www.promega.com
1. Introduction
1.A. Maxwell
®
RSC Instrument Purification Procedure
The Maxwell
®
Rapid Sample Concentrator (RSC) Instrument provides automated nucleic acid purification
for a range of research sample types. The Maxwell
®
RSC Instrument is designed for use by laboratory
professionals. The purification methods use sample lysis and binding to paramagnetic particles as the
primary separation principle. Up to 16 samples can be prepared in a single run.
The automated purification performed by the Maxwell
®
RSC Instrument include:
• Sample lysis in the presence of a specially formulated Lysis Buffer
• Binding of nucleic acids to paramagnetic particles
• Washing of the bound target molecules away from other cellular components
• Elution of the product
The instrument is controlled through a graphical user interface running on a tablet PC. The Maxwell
®
RSC
Instrument has the ability to record and report sample tracking and method run data. An optional Bar Code
Reader (available separately; Cat.# AS4010) can be used with the Maxwell
®
RSC Instrument to start
method runs and capture bar code information for samples and reagents. The Maxwell
®
RSC Instrument
provides reports of the data gathered for instrument operations; reports can be printed and exported to a
storage location or a USB drive for transfer to a separate computer. To start a run the user selects the
appropriate method or scans the bar code of the reagent kit to be processed; this selects the appropriate
protocol to be run. After entering the sample tracking information, the user follows the recommended
protocol for the Maxwell
®
RSC reagent kit and prepares the deck tray of the instrument as instructed. The
Deck Tray containing prepared cartridges is placed into the instrument, and the method is automatically
run. Use of the instrument does not require any special training. However, training is available as part of
Operational Qualification, which is offered separately (see Section 9.B)
1.B. Product Use
The Maxwell
®
RSC Instrument is exclusively designed to be used with Maxwell
®
RSC reagent kits to
perform automated isolation of nucleic acids.
The Maxwell
®
RSC is for research use only.
