PerkinElmer LS 55, User Manual

Page: 60 / 202

60 . LS 55 User’s Guide
10. Start a TimeDrive using suitable wavelengths for the fluorescent dye used,
using slit widths of 10 nm (excitation) and 5 nm (emission).
Duration should be 2-3 minutes.
11. During data collection, adjust the two (1/8") Hex screws shown in
Figure 41 until the maximum signal is observed.
Figure 41 Adjustment screws
12. Remove the accessory from the LS 55 sample area and tighten the locking
screws shown in Figure 39.
13. Re-fit the front plate, as shown in Figure 38.
14. Re-insert and re-clamp the tubing using the tubing clamp screw, as shown in
Figure 38.
Operation
The Sipper accessory can be controlled manually using the LS 55 Status application.
The Scan and Concentration applications include full sipper control. The Sipper
parameters (pump time, wait time, purge time and direction) are set in the
application so that when the Sipper is started by pushing the Sipper arm upwards,
sampling and measurement are automatically triggered.
Manual control
1. Start the
LS 55 Status
application from the FL WinLab software.