PerkinElmer LS 55, User Manual

Page: 63 / 202

Accessories: Installation and Use . 63
LC Flowcell
Figure 42 The LC Flowcell
Description
The LC Flowcell accessory (L2250138) enables you to collect chromatographic data
using the LS 55. The flowcell has internal dimensions of 1.5 x 1.5 x
10 mm (measured volume approximately 20 microlitres) and is manufactured from
synthetic fused silica, giving optical performance down to 200 nm.
Inlet and outlet tubes are made of an inert polymer material (FEP) which ensures
biocompatibility.
NOTE: FEP tubing has low structural strength and thus poor resistance to back
pressure, so care should be taken when placing the LS 55 with LC Flowcell
into a series of detectors. The LS 55 should be located at the end of the
chain to reduce back pressure.
Installation
The LC Flowcell accessory must be aligned before use, to ensure that the center of
the flowcell sits correctly at the optical focus of the LS 55:
1. Loosen the two front plate fixing screws and remove the front plate, gently
feeding the inlet and outlet tubes through the grommets on the front plate, as
shown in Figure 43.