PerkinElmer LS 55, User Manual

Page: 43 / 202

Accessories: Installation and Use . 43
Screw-fitting Flowcell
Figure 21 Screw-fitting flowcell
Description
The screw-fitting flowcell (B0631133) is intended for automated flow-through, for
example using an autosampler.
NOTE: The flowcell is not intended for HPLC, or other high resolution applications
(see instead the
LC Flowcell
on page 63).
The flowcell has three main advantages:
• The screw fittings allow tight connections to be made with autosamplers or
automated flow systems where high sample throughput requires high reliability
due to the unattended nature of data collection. Use the PerkinElmer Flange
toolkit, Part number B3000001.
• The internal volume of the flowcell (the internal chamber has dimensions
3x3x10 mm, a volume of 90
μ L) is almost perfectly matched to the light beam
geometry of the LS 55 (the measured volume is 3x3x9 mm, a volume of 81 μ L).
This means that this flowcell does not suffer from the sensitivity losses
normally associated with flowcells, but gives excellent sensitivity, equivalent to
a normal cuvette.
• The flowcell fits into a standard cuvette holder, allowing the flowcell to be
thermostatted (as detailed in
Installing Thermostatting Tubing to Cellholders
on
page 45).