PerkinElmer LS 55, User Manual

Page: 18 / 202

18 . LS 55 User’s Guide
Specifications of the LS 55
Principle: Computer controlled ratioing luminescence spectrometer with the
capability of measuring fluorescence, phosphorescence, chemiluminescence and
bioluminescence.
Source: Xenon discharge lamp, equivalent to 20 kW for 8 μ s duration. Pulse width
at half height <10 μ s.
Sample detector: Gated photomultiplier with modified S5 response for operation up
to around 650 nm. Red-sensitive R928 photomultiplier can be optionally fitted for
operation up to 900 nm.
Reference detector: Photodiode for operation up to around 900 nm.
Monochromators: Monk-Gillieson type monochromators cover the following ranges:
• Excitation 200-800 nm with zero order selectable.
• Emission 200-650 nm with standard photomultiplier with zero order
selectable, 200-900 nm with optional R928 photomultiplier.
Synchronous scanning is available with constant wavelength or constant energy
difference.
Wavelength accuracy: +1.0 nm
Wavelength reproducibility: +0.5 nm
Spectral bandpass: The excitation slits (2.5-15.0 nm) and emission slits
(2.5-20.0 nm) can be varied and selected in 0.1 nm increments.
Phosphorescence mode: Delay and gate times can be varied to measure
phosphorescence. Minimum total period 13.0 ms (50 Hz)
Scanning speed: Scanning speed can be selected in increments of 1 nm for
10-1500 nm/min. Time-dependent data can also be collected.
Emission filters: Computer selectable cut-off (high-pass) filters at 290, 350, 390,
430 and 515 nm, a blank (to act as shutter), a 1% T attenuator and clear beam
position.
Sensitivity: Signal to noise is 500:1 r.m.s., using the Raman band of water with
excitation at 350 nm, excitation and emission bandpass 10 nm.
Standard cellholder: A single position water thermostattable holder for 10 mm
cuvettes.