PerkinElmer LS 55, User Manual

Page: 58 / 202

58 . LS 55 User’s Guide
The measured volume of the sipper flowcell is approximately 1.5 x 1.5 x 10 mm.
The maximum measured volume of the LS 55 (with wide open excitation and
emission slits) is approximately 3 x 3 x 10 mm, so the absolute sensitivity when
using the Sipper accessory would be around a factor of 4 times less sensitive than
when using a cuvette. In spite of this loss, the Sipper accessory represents a much
more sensitive option for high sampling throughput than for example a plate
reader, where sensitivity would be at least a factor of 20 less sensitive (even for
dedicated plate reader instruments).
Installation and maintenance
The Sipper accessory must be aligned before use, to ensure that the center of the
flowcell sits correctly at the optical focus of the LS 55. Installation is carried out as
follows:
1. If the tubing is clamped in the switch lever, loosen the tubing clamp screw, as
shown in Figure 38, and pull out the tubing.
Figure 38 The front of the Sipper
2. Loosen the two front plate fixing screws, as shown in Figure 38, and remove
the front plate.
3. Loosen the two mount plate locking screws, as shown in Figure 39.
This enables the mount plate to move freely during alignment.