114.
Series 200a Fl User’s Guide
Table 5-2 Characteristics of Organic Solvents
Ignition Point
Polarity
E
2
(Al
2
O
3
)
Viscosity
(cP20
°
C)
Refractive
Index
UV Cut
off (nm)
Flash
Point (
°
C)
°
C
Lower
Limit
Upper
Limit
Vapor
Density
(Air: 1)
Boiling
Point (
°
C)
Specific
Gravity
(Water: 1)
Fluoroalkanes
-0.25
1.25
n-Pentane
0.00
0.23
1.358
210
< -40
308.9
1.5
7.8 2.5 36.1 0.6
Hexane
0.00
1.375
210
-21.7
233.9 1.2 7.5 3.0 68.9 0.7
Isooctane
0.01
1.404
210
Petroleum ether
0.01
0.3
210
n-Decane
0.04
0.92
1.412
46.1 207.8 0.8 5.4 4.9 173.9
0.7
Cyclohexane
0.04
1.00
1.427
210
-20 260 1.3 8 2.9 81.7 0.8
Cyclopentane
0.05
0.47
1.406
210
Diisobutylene
0.06
1.411
210
i-Pentene
0.08
1.371
-17.8 272.8 1.5 8.7 2.4 30
0.7
Carbon disulfide
0.15
0.37
1.626 380 -30 100 1.3
44 2.6 46.1 1.3
Carbon tetrachloride
0.18
0.97
1.466
265
Amyl chloride
0.26
0.43
1.413
225 12.8
343.3
1.6 8.6 3.7
106.1 0.9
Butyl chloride
0.26
1.436
220
-9.4 460 1.8 10.1 3.2
0.9
0.81
†
o-17.2
463.9 1.0 6.0 144.4
Xylene
0.26
0.61
†
~
1.50
290 m-25
527.8 1.1 7.0 3.7
138.9 0.9
0.64
†
p-25 528.9 1.1 7.0 138.3
i-Propyl ether
0.28
1.368
220
-27.8 443.3 1.4 21 3.5
68.9 0.7
i-Propyl chloride
0.29
0.37
1.378
225 -32.2
593.3
2.8 10.7 2.7 35
0.9
Toluene
0.29
0.33
1.496
285
4.4
536.1 1.4 6.7 3.1 110.6
0.9
n-Propyl chloride
0.30
0.59
1.389
225 <
-17.8
2.6 11.1 2.7 46.1 0.9
Chlorobenzene
0.30
0.35
1.525
32.2 637.8 1.3 7.1 3.9
132.2 1.1
Benzene
0.32
0.80
1.501
280
-11.1 562.2 1.4 7.1 2.8 80
0.9
Ethyl bromide
0.37
0.65
1.424
511.1
6.7
11.3
3.8
37.8
1.4
Ethyl ether
0.38
1.353
220 -45 180 1.9
48 2.6 35 0.7
Ethyl sulfide
0.38
0.23
1.442
290
Chloroform
0.40
0.45
1.443
245
Methylene chloride
0.42
0.57
1.424 245 -50 518.9
3.8 15.4 2.2 38.5 0.9
Methyl i-butyl ketone
0.43
0.44
1.394
330
Tetrahydrofurane
0.45
1.408
220
-14.4 321.1 2 11.8 2.5 66.1 0.9
Ethylene dichloride
0.49
1.445 230 13.3
412.3
6.2
16 3.4
83.9 1.3
Methyl ethyl ketone
0.51
0.79
1.381 330 -6.1
515.6
1.8
10 2.5 80 0.8
i-Nitropropane
0.53
1.400
380
48.9 420.6 2.6
3.1 131.1
1.0
Acetone
0.56
1.359
220
-17.8 537.8 2.6 12.8 2.0 56.7 0.8
Dioxane
0.56
0.32
1.422
260
12.2 180 2.0 22
3.0 101.1 1.0
Ethyl acetate
0.58
1.54
1.370 260 4.4
460 1.8 8 3.5 90 0.9
Methyl acetate
0.60
0.45
1.362 210 -10 501.7
3.1
16 2.6 60 0.9
Amyl alcohol
0.61
0.37
1.410
32.8
300 1.2
10.0 3.0
137.8 0.8
Dimethyl sulfoxide
0.62
4.1
Aniline
0.62
2.24
1.586
70
617.2 1.3
3.2 184.4 1.0
Dimethyl amine
0.63
4.4
1.387
275
< -17.8
312.2
1.8
10.1 2.5 56.7 0.7
Nitromethane
0.64
0.38
1.394
380 35 418.3
7.3 2.1
101.1 1.1
Acetonitrile
0.65
0.67
1.344
210 5.6 1.4
81.7
0.8
Pyridine
0.71
0.37
1.510
305
20
1.8
12.4
2.7
115
1.0
Butyl cellosolve
0.74
0.94
220
i-Propanol n-Propanol
0.82
1.38
210 11.7
398.9
2.0
12 2.1
82.8 0.8
Ethanol
0.88
2.3
1.361
210
12.8 422.8 4.3 19
1.6 78.3 0.8
Methanol
0.95
1.20
1.329
210
11.1 463.9 7.3 36
1.1 63.9 0.8
Ethylene glycol
1.11
0.60
1.427 210 111.1
412.8
3.2 197.2 1.1
Acetic acid
Large
19.9
1.372
Water
Large
1.26
1.333
Salts & buffer
Larger
* L.R.Snydar, Dekker. “Principles of Adsorption Chromatography”
†
The Handbook of Chemistry, 2nd ed., The Chemical Society of Japan
Solvent with low boiling point as shown in cannot be used.
Summary of Contents for 200A Series
Page 1: ...SERIES 200A FLUORESCENCE DETECTOR User s Guide LIQUID CHROMATOGRAPHY ...
Page 5: ...Introduction ...
Page 6: ......
Page 15: ...Safety Practices ...
Page 16: ......
Page 17: ......
Page 33: ...Safety Practices 33 Figure 2 2 Overview of the warning labels on the front of the instrument ...
Page 35: ...System Description 35 System Description ...
Page 36: ...36 Series 200a Fi User s Guide ...
Page 55: ...Installation 55 Installation ...
Page 56: ...56 Series 200a Fi User s Guide ...
Page 66: ...66 Series 200a Fi User s Guide ...
Page 67: ...Operation 67 Operation ...
Page 68: ...68 Series 200a Fi User s Guide ...
Page 117: ...Maintenance 117 Maintenance ...
Page 118: ...118 Series 200a Fl User s Guide ...
Page 154: ...154 Series 200a Fl User s Guide ...
Page 155: ...Troubleshooting ...
Page 156: ...156 ...
Page 162: ...162 Series 200a Fl User s Guide ...
Page 163: ...Appendix 163 Appendix ...
Page 164: ...164 Series 200a Fl User s Guide ...
Page 170: ...170 Series 200a Fl User s Guide ...