PerkinElmer 200A Series, User Manual

Page: 101 / 172

Operation
101
Press numeric keys 2 5 0 to 9 0 0 and then ENT key.
™
Setting range: 250 to 900 nm
It is recommended to set the same wavelength as was set for Em of the measuring
wavelengths.
™
Initial (default) value: blank
The cursor next moves to BASE.
IV. Set the BASE (baseline processing).
<Step setting screen (BASE indicated)>
TIME Ex Em BASE PMT
0.0 250 350 1
1
Press a numeric key (1 or 2; usually 1) and then ENT key.
™
1: AUTO ZERO is executed. Data baseline is set to the zero point of the
recorder.
™
2: HOLD is executed. Baseline is held so that it doesn’t fluctuate. HOLD function
is released by executing AUTO ZERO.
™
Initial (default) value: 1
V. Set the PMT (photomultiplier voltage).
<Step setting screen (PMT indicated)>
TIME Ex Em BASE PMT
0.0 250 350 1 3 1
Press a numeric key 1 to 5 and then ENT key.
VI. When the setting for the first step is completed, return to the TIME setting on
step setting screen.
™
If some parameters will be changed, advance to the second and subsequent steps.
If parameters will not be changed, advance to the final step setting.
e. Setting of second and subsequent steps of time program:
i. Set the TIME to be changed.
<Step setting screen (TIME indicated)>
TIME Ex Em BASE PMT
_
10.0 1