PerkinElmer 200A Series, User Manual

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Maintenance . 129
1.3 405
1.4 410
1.5 415
1.6 430
1.8 450
2.0
NOTE:
The above time program shows an example where the minimum FL value was obtained at 380 nm
and the maximum at 400 nm. For the top Em of the program, set a wavelength that includes the
wavelengths where the maximum and minimum FL values were obtained. (For instance, if the
maximum FL value was obtained at a wavelength of 397 nm, then the wavelength at 1.1 minutes
should be set at 397 nm.)
Read out the value corresponding to “S” from the spectrum obtained with the time program.
Fig. 6-4 Calculate the Raman Peak of Water
When using the recorder, calculation can be made using the length (L in mm) of S on the chart.
L: length of S (mm)
X: ATT setting of data processor used in measurement
Refer to previous subsections (4) through (6) for measurement and calculation of noise and
calculation of S/N ratio.
8. Measurement of Drift: Drift is measured by using the data and baseline obtained from the
Raman spectrum of water (measurement conditions are the same as in preceding subsection
(4); but measuring time is 15 minutes). For the sake of simplicity, calculation can be performed
on the assumption that the output signal is zero at the emission wavelength of 450 nm.