Operation
91
7.
CHECK (Xe lamp energy check)
Energy level of the xenon lamp can be checked using the xenon lamp beam at 254 nm.
a.
Press the GLP key.
b.
The following display will be presented.
<GLP initial screen>
GLP
(LOGBOOK=1, KEY LOCK=2,
4
WL CHECK=3, Xe CHECK=4)
Select Xe CHECK.
Press numeric key 4 and then ENT key.
If the Xe lamp is turned off, the system will return to the 1st monitor screen without performing
an energy check.
c.
The Xe lamp energy check screen appears and an energy check will be performed.
<Xe lamp energy check screen> (energy check is in progress)
CHECKING LAMP ENERGY
(Xe)
d.
The result of Xe lamp energy check will be displayed automatically.
<Xe lamp energy check result>
Xe ENERGY
1200
Press the ENT or ESC key.
If 5 minutes or more elapses without pressing ENT or ESC key, the system will return
automatically to the 1st monitor screen.
e.
When the Xe lamp energy check is finished, the system returns to the 1st monitor screen.
NOTE:
When the temperature of the Xe lamp is high, the sealed gas pressure will fluctuate and the lamp may be
difficult to ignite. Before restarting the instrument or re-lighting the lamp, wait at least five minutes for
the lamp to cool down after turning it off.
Spectrum Measurement
Set the wavelength scan range (maximum and minimum values) for excitation (Ex) and emission (Em),
then measure and record the spectral data for each.
Carry out recording of spectral data with sample contained in the cell (sample not flowing, but
stationary in cell).
Summary of Contents for 200A Series
Page 1: ...SERIES 200A FLUORESCENCE DETECTOR User s Guide LIQUID CHROMATOGRAPHY ...
Page 5: ...Introduction ...
Page 6: ......
Page 15: ...Safety Practices ...
Page 16: ......
Page 17: ......
Page 33: ...Safety Practices 33 Figure 2 2 Overview of the warning labels on the front of the instrument ...
Page 35: ...System Description 35 System Description ...
Page 36: ...36 Series 200a Fi User s Guide ...
Page 55: ...Installation 55 Installation ...
Page 56: ...56 Series 200a Fi User s Guide ...
Page 66: ...66 Series 200a Fi User s Guide ...
Page 67: ...Operation 67 Operation ...
Page 68: ...68 Series 200a Fi User s Guide ...
Page 117: ...Maintenance 117 Maintenance ...
Page 118: ...118 Series 200a Fl User s Guide ...
Page 154: ...154 Series 200a Fl User s Guide ...
Page 155: ...Troubleshooting ...
Page 156: ...156 ...
Page 162: ...162 Series 200a Fl User s Guide ...
Page 163: ...Appendix 163 Appendix ...
Page 164: ...164 Series 200a Fl User s Guide ...
Page 170: ...170 Series 200a Fl User s Guide ...