PerkinElmer 200A Series, User Manual

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Operation
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WARNING
Inflammation or Injury due to Toxic or Corrosive Solvent
When using a toxic or corrosive solvent, be careful not to incur a
physical inflammation or injury.
For details of the properties of each solvent and how to handle it, refer
to the relevant Material Safety Data Sheets (MSDS). Be sure to handle
each solvent properly.
d. Wear proper protective clothes (e.g., safety goggles) so that
a solvent will not come into direct contact with the skin.
e. Ventilate the laboratory room adequately to prevent
accidental inhalation of harmful solvent vapor.
Selection of Mobile Phase and Cautions on Handling
1. Be careful about restriction on use due to characteristics and temperature of mobile phase to
be used. (See Table 5-2 Characteristics of Organic Solvents)
2. When performing high sensitivity analysis with a UV detector, use solvents whose UV
absorption characteristics are especially small. Use solvents for liquid chromatograph
commercially available.
Absorption characteristics of special grade reagents such as methanol, ethanol and acetonitrile
generally and widely used are greatly different in the short wavelength range, depending on
the manufacturing lot and the manufacturer.
Be careful especially when performing high sensitivity analysis.
3. Degas the mobile phase before use.
¾
It is recommended to use a degasser because organic solvents have a specially high
content of air and a poor baseline may result.
A malfunction of the check valve and loss of column efficiency may also occur.
Degasser is especially required for mixing solvents for gradient use. Degas the
solvents before use. See the section in this chapter on
Degassing Mobile Phase.
4. Mobile Phase
When using mobile phase for liquid chromatograph, since the connections of the tubing for the
pump and separation column use stainless steel, solvents or reagents that may corrode
stainless steel should be avoided.
For example, solutions which contain halogen ions such as HCl, KCl, NaCl, NH
4
Cl.
Reagents which may be restricted are shown in Table 5-1; use the table as a rough standard.