CONTENTS
1 Getting Started FLUOVIEW 1-1
1-1 Basic Operations..............................................................................1-1
1-1-1 Microscope.................................................................................................. 1-1
1-1-2 General Mouse Operation Procedures ...................................................... 1-13
1-1-3 Names of Major Panel and Window Controls and Their Functions............ 1-14
1-2 Outline of LSM Observation Procedures .....................................1-16
1-2-1 Turning Power On ..................................................................................... 1-18
1-2-2 Starting the Software................................................................................. 1-22
1-2-3 Focusing on the Specimen........................................................................ 1-24
1-2-3-1 Combination with BX ....................................................................................... 1-24
1-2-3-2 Combination with AX ....................................................................................... 1-26
1-2-3-3 Combination with IX ........................................................................................ 1-28
1-2-3-4 Combination of IXBP (Bottom Port) ................................................................ 1-29
1-2-4 Setting the LSM Light Path........................................................................ 1-31
1-2-4-1 Combination with Upright Microscope (BX)..................................................... 1-31
1-2-4-2 Combination with Inverted Microscope (BX50WI)........................................... 1-33
1-2-4-3 Combination with Upright Microscope (AX)..................................................... 1-35
1-2-4-4 Combination with Upright Microscope (AX70A) .............................................. 1-37
1-2-4-5 Combination with Inverted Microscope (IX)..................................................... 1-39
1-2-4-6 Combination of IXBP (Bottom Port) ................................................................ 1-41
1-2-5 Selecting the Dyeing Method..................................................................... 1-43
1-2-6 Selecting the Filters................................................................................... 1-46
1-2-7 Selecting the ND Filters............................................................................. 1-48
1-2-8 Setting the Observation Condition............................................................. 1-49
1-2-8-1 Setting the Objective Magnification ................................................................. 1-49
1-2-8-2 Setting the Zoom Ratio to 1X .......................................................................... 1-50
1-2-8-3 Setting the Channels ....................................................................................... 1-50
1-2-8-4 Setting the Highest Scan Speed...................................................................... 1-50
1-2-8-5 Setting the XY Observation Mode ................................................................... 1-51
1-2-8-6 Repeated Scanning Operation ........................................................................ 1-52
1-2-8-7 Setting the Cross-section to be Observed....................................................... 1-52
1-2-8-8 Setting the Area to be Observed ..................................................................... 1-53
1-2-8-9 Setting a Lower Scan Speed ........................................................................... 1-54
Summary of Contents for FLUOVIEW FV500
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