Olimpus FLUOVIEW FV500, User Manual

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Getting Started FLUOVIEW/
Outline of LSM Observation Procedures
1-35 Page
1-2-4-3 Combination with Upright Microscope (AX)
1. From the page tabs on the bottom right of the [Acquire] panel, select the [Optics]
sub-panel.
Fig. 1-8 [Optics] Sub-panel
2. Select the <LSM> button in the [Light Path] group box.
The <LSM> button looks pushed in to indicate that it is selected.
(When scanning is started while the <BI> button is selected, the LSM light path is
selected automatically. It is switched back to the visual observation automatically
when scanning completes.)
3. Set a cube in the Universal vertical illuminator mirror cube housing (1) by pressing
the desired cube conversion button (6) on the U-SHTR hand switch.
4. When the FV5-ANA analyzer (2) is in use, disengage it from the light path by
setting the switch to the pulled-out position.
5. When only fluorescence observation is required, disengage the FV5-DICT
transmitted light DIC slider (3) by setting the switch to the pulled-out position. When
transmitted light differential interference observation or simultaneous fluorescence
+ transmitted light differential interference observation is required, engage the
FV5-DICT and the optimum transmitted light DIC slider for the objective in the light
path by operating the universal condenser (5).
6. For transmitted light observation, disengage any filter (4) from the light path.