Getting Started FLUOVIEW/
Basic Operations
1-11
Page
(2) Cube turret
Switch the cubes for fluorescence observation by rotating this turret.
•
For visual fluorescence observation, engage the specified cube in the light
path.
•
For laser microscopy or for visual transmitted light observation, rotate the
turret so that the index indicates
. (This sets the cube turret to an idle
position without cube.)
(3) Analyzer FV5-ANI
Polarizer for use in DIC or polarized light observation.
•
For visual transmitted DIC observation or transmitted polarized light
observation, push in the lever to engage the analyzer in the light path.
•
For laser microscopy, be sure to disengage the analyzer from the light path by
pulling the lever out. High quality image cannot be obtained if the analyzer is
left engaged in the light path.
(4) Transmitted light DIC slider FV5-DICT (optional)
Prism for use in DIC observation.
•
For laser DIC observation or visual transmitted light DIC observation, engage
If fluorescence observation using laser is performed with the FV5-DICT
engaged in the light path, the resolution may deteriorate somewhat. It is
recommended to disengage the FV5-DICT from the light path when
performing only the fluorescence observation using laser.
With transmitted DIC observation using a water-immersion objective,
reduce the field iris diaphragm of the microscope until it circumscribes
the field of view. Otherwise, the contrast may deteriorate. (This applies
to both visual and laser DIC observations.)
NOTE
Summary of Contents for FLUOVIEW FV500
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