BIO RAD CFX384, Instruction Manual

Page: 66 / 158

CFX96 and CFX384 Systems Manual
57
NOTE: Press the alphanumeric keys to enter a Gradient Range spanning from 1 to
24
o
C.
TIP: Once a step has a gradient, you can edit the upper and lower temperatures in
the graphic or text view without opening the
Options screen.
5. The GOTO step instructs the thermal cycler to repeat a set of steps in a loop to create
the cycles in the PCR experiment. Select a GOTO step; press the arrow keys to select
and then edit the step number in a GOTO step or to change the number of repeats.
Entering a Protocol Name:
• When creating a new protocol, you have the option to save it with a name. Use the
arrow keys to navigate to the Protocol
Name box and then press the alphanumeric
keys multiple times to enter a letter or number to type a new protocol name
(Figure 47 on page 56).
• Press ENTER to accept the name.
Changing run parameters:
• To change the default sample volume, select the sample volume box ( Vol ) (Figure 47
on page 56). Use the alphanumeric keys to enter a new sample volume in microliters.
The sample volume you enter determines the temperature control mode that is used
during a run
TIP: Entering a sample volume from 1 to 50 selects Temperature Control mode,
which is the standard mode. Entering zero (0) selects Block mode. Temperature
mode is the recommended mode because it most accurately represents the actual
sample temperature.
• To change the default lid temperature, select the lid temperature box ( LID ) by
pressing the arrow keys (Figure 47 on page 56). Use the alphanumeric keys to enter
a new temperature. For the CFX96 system, use a lid temperature of 105
o
C; for the
CFX384 system use a lid temperature of 95
o
C
NOTE: Heating the lid prevents condensation in the sealed reaction vessels.
Running the Protocol:
1. To run the protocol, click the Done (F2) button in the Protocol window (Figure 47 on page
56).
TIP: Alternatively, click the RUN command key to start the run without saving or
editing the name of the protocol.