BIO RAD CFX384, Instruction Manual

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CFX96 and CFX384 Systems Manual
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Scan Mode
The CFX96 system excites and detects fluorophores in six channels. The CFX384 system
excites and detects fluorophores in five channels. Both systems use multiple data acquistion
scan modes to collect fluorescence data from during a run.
Select one of these scan modes int he Plate Editor window toolbar:
• All Channels. Includes channels 1 through 5 on the CFX96 system or channels 1
through 4 on the CFX384 system
• SYBR/FAM only. Includes only channel 1, and provides a fast scan
• FRET. Includes only the FRET channel and provides a fast scan
Select Fluorophores Window
The Select Fluorophores window lists fluorophores that can be selected to load into the Plate
Editor well loading controls. To open the Select Fluorophores window, click the
Select
Fluorophores button on the right side of the Plate Editor.
NOTE: The fluorophores listed depend on the scan mode; when SYBR/FAM only is
chosen, only channel 1 fluorophores are shown in the Select Fluorophores window.
NOTE: You cannot add or remove fluorophores in this list; you must calibrate the
new fluorophores on an instrument in the Calibration Wizard (page 131). After
calibration, the new fluorophore is added to the Select Fluorophore window.
Click the Selected check box next to the fluorophore name to add or remove the fluorophores
to the list on the right side of the Plate Editor window.
In this example, SYBR is selected from the list of available fluorophores (Figure 40).
Figure 40. Select Fluorophores window.
• Click the Color box next to the fluorophore name and select a new color to represent
each fluorophore in the Plate Editor window and Data Analysis charts
NOTE: Before beginning the run, the software verifies that the fluorophores you
specified in the plate are calibrated on that instrument. You cannot run a plate if it
includes fluorophores that have not been calibrated on that instrument.