BIO RAD CFX384, Instruction Manual

Page: 129 / 158

Gene Expression Analysis
120
The standard error for the normalization factor in the normalized expression formula is shown
here:
Where:
• n = Number of reference targets
• SE = Standard error
• NF = Normalized expression
• RQ = Relative quantity
The standard error for normalized gene of interest (GOI) formula is shown here:
Where:
• SE = Standard error
• GOI = Gene of interest (one target)
• NF = Normalization factor
• n = Number of reference targets
SE NF
n
NF
n
SE RQ
sample (Ref 1)
n SE RQ
sample (Ref 1)
×
------------------------------------------------------
⎝ ⎠
⎛ ⎞
2 SE RQ
sample (Ref 2)
n SE RQ
sample (Ref 2)
×
------------------------------------------------------
⎝ ⎠
⎛ ⎞
2
…
SE RQ
sample (Ref n)
n SE RQ
sample (Ref n)
×
------------------------------------------------------
⎝ ⎠
⎛ ⎞
2
+ + +
×
=
SE GOI
n
GOI
n
SE NF
n
NF
n
------------------
⎝ ⎠
⎛ ⎞
2
SE GOI
GOI
-------------------
⎝ ⎠
⎛ ⎞
+
2
×
=