Plates
50
•
Select the sample as a control sample for gene expression data analysis by clicking
the box in the
Control
column next to the name for that sample
Click the
Show Analysis Settings
box in the Experiment Settings window to view or change
analysis parameters applied in the Gene Expression tab.
To adjust target parameters:
•
Click a cell in the
Color
column to change the color of the targets graphed in the
Gene Expression chart
•
Enter a number for the efficiency of a target. The software will calculate the relative
efficiency for a target using
Auto Efficiency
if the data for a target includes a
standard curve. Alternatively, type a previously determined efficiency
To adjust the settings for a sample in the Samples tab:
•
Click a color in the
Color
column to change the color of the samples graphed in the
Gene Expression chart
•
Click a box in the
Show Graph
column to show the sample in the Gene Expression
chart using a color that is selected in the
Color
column
Sample Name Grouping Option
Loading
Collection Names
in the wells enables samples to be analyzed in one of four
configurations defined by the Sample Name Grouping Option. These options are available
from the pull down menu in the Experiment Settings tab.
•
Target vs. Sample
•
Target vs. Collection
•
Target vs. Sample_Collection
•
Target vs. Collection_Sample
Well Groups Manager Window
Well groups divide a single plate into subsets of wells that can be analyzed independently in
the Data Analysis window. Once well groups are set up, select one in the Data Analysis
window to analyze the data in an independent group. For example, set up well groups to
analyze multiple experiments run in one plate, or to analyze each well group with a different
standard curve.
NOTE: The default well group is
All Wells
.
Create Well Groups
To create well groups in the Well Groups Manager window, follow these instructions:
1. Click the
Well Groups
button in the toolbar of the Plate Editor.
2. Click
Add
to create a new group. The pull-down menu shows the group name as
Group
1
for the first group.
3. Select the wells that will compose the well group in the plate view by clicking and
dragging across the group of wells. Selected wells turn blue in color (Figure 43).
4. (Optional) Change the name of the group by selecting the group name in the pull-down
menu and typing a new name.
Summary of Contents for CFX384
Page 9: ...Table of Contents viii ...
Page 49: ...Protocols 40 ...
Page 89: ...Data Analysis Overview 80 ...
Page 149: ...Resources 140 ...
Page 157: ...Index 148 ...
