Gene Expression Analysis
110
Figure 104 shows the Gene Study window, including the Study Setup and Study Analysis tabs.
Figure 104. Gene Study window.
Study Setup Tab
Before importing data into a Gene Study, do the following in the Data Analysis window:
•
Check that samples that contain the same content are named with the same name.
In a Gene Study, the software assumes that wells with the same Target or Sample
name contain the same samples
•
Adjust the baseline and threshold (C(t)) in the Quantitation tab to optimize the data in
each experiment before you add them to a Gene Study
•
Select the well group you want to include in the Gene Study
The Study Setup tab (Figure 104) shows a list of all the experiments in the Gene Study.
•
Add experiments.
Click the
Add Data Files
button to select a file from a browser
window. To quickly add experiments to a Gene Study, drag the data files (.pcrd
extension) to the Gene Study window
TIP: In order to show data from one well group in the Gene Study, that group must
be selected before importing the Data file
•
Remove experiments from this Gene Study.
Select one or more files in the list and
click
Remove
•
Add notes about the Gene Study.
Type in the Notes box to add comments about the
files and analysis in this Gene Study
Summary of Contents for CFX384
Page 9: ...Table of Contents viii ...
Page 49: ...Protocols 40 ...
Page 89: ...Data Analysis Overview 80 ...
Page 149: ...Resources 140 ...
Page 157: ...Index 148 ...
