BIO RAD CFX384, Instruction Manual

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Running Experiments
22
The Experiment Setup window opens with the Protocol tab in front (Figure 23). To open
another tab, click that tab or click
Prev and Next buttons at the bottom of the window.
Figure 23. Experiment Setup window, including the Protocol, Plate, and Start Run tabs.
Protocol Tab
The Protocol tab shows a preview of the selected protocol file loaded in the Experiment Setup
(Figure 23). A protocol file contains the instructions for the instrument temperature steps, as
well as instrument options that control the ramp rate and lid temperature.
Select one of the following options to select an existing protocol, create a new protocol, or edit
the currently selected protocol:
• Create New button. Open the Protocol Editor to create a new protocol
• Select Existing button. Open a browser window to select and load an existing protocol
file (.prcl extension) into the Protocol tab
• Express Load pull-down menu. Quickly select a protocol to load it into the Protocol tab
TIP: To add or delete protocols in the Express Load menu, add or delete files (.prcl
extension) in the ExpressLoad folder. To locate this folder, select Tools > User
Data Folder in the menu bar of the main software window
• Edit Selected button. Open the currently selected protocol in the Protocol Editor
End Point Only Runs
To run a protocol that contains only an end point data acquisition step, select Options > End
Point Only Run from Options in the menu bar of the Experiment Setup window. The default
end point protocol, which includes two cycles of 60.0°C for 30 seconds, is loaded into the
Protocol tab.