CFX96 and CFX384 Systems Manual
127
•
Analysis Mode.
Select the default base lining method for the analysis mode. Choose
Baseline Subtracted Curve Fit, No Baseline Subtraction, or Baseline Subtracted
•
C(t) Determination Mode.
Select between Regression mode or Single Threshold mode
to determine how C(t) values are calculated for each fluorescence trace
•
Log View.
Select
On
to show a semi-logarithmic graph of the amplification data. Select
Off
to show a linear graph
For the allelic discrimination data, select the following settings:
•
Display Mode.
Select
RFU
to show the data as a graph of the RFU, or select
Threshold
Cycle
to show a graph of threshold cycles
•
Normalize Data.
This selection is only available when RFU is selected. Select
No
to
show unnormalized data. Select
Yes
to normalize the data to the control sample
For the end point data, select the following settings. Select the number of end cycles to
average when calculating the end point calculations:
•
PCR.
Enter a number of cycles for PCR to average the end cycles for quantitation data
(default is 5)
•
End Point Only Run.
Enter a number of cycles for End Point Only Run to average the
end cycles for end point data (default is 2)
Gene Expression Tab
Select the
Gene
Expression
tab in the User Preferences window to specify the default
settings for a new Gene Expression data file.
Figure 117. Gene Expression tab in the User Preferences window.
Specify the default settings for a new Gene Expression data file:
•
Relative to.
Select a control or zero. To graph the gene expression data originating at 1
(relative to a control), select
Control
. When you assign a control sample in the
Experiment Setup window, the software automatically defaults to calculate the data
relative to that control. Select
Relative to zero
to instruct the software to ignore the
Summary of Contents for CFX384
Page 9: ...Table of Contents viii ...
Page 49: ...Protocols 40 ...
Page 89: ...Data Analysis Overview 80 ...
Page 149: ...Resources 140 ...
Page 157: ...Index 148 ...
