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LIMITATION OF WARRANTIES / LIMITED REMEDY
EXCEPT AS EXPRESSLY STATED IN A LIMITED WARRANTY SECTION OF INDIVIDUAL PRODUCT PACKAGING, 3M DISCLAIMS ALL EXPRESS AND IMPLIED
WARRANTIES, INCLUDING BUT NOT LIMITED TO, ANY WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE. If any 3M Food Safety
Product is defective, 3M or its authorized distributor will, at its option, replace or refund the purchase price of the product. These are your exclusive
remedies. You must promptly notify 3M within sixty days of discovery of any suspected defects in a product and return it to 3M. Please call Customer
Service (1-800-328-1671 in the U.S.) or your official 3M Food Safety representative for a Returned Goods Authorization.
LIMITATION OF 3M LIABILITY
3M WILL NOT BE LIABLE FOR ANY LOSS OR DAMAGES, WHETHER DIRECT, INDIRECT, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES,
INCLUDING BUT NOT LIMITED TO LOST PROFITS. In no event shall 3M’s liability under any legal theory exceed the purchase price of the product
alleged to be defective.
STORAGE
Store unopened 3M Petrifilm RYM Plate pouches refrigerated or frozen (-20 to 8°C / -4 to 46°F). Just prior to use, allow unopened pouches to come
to room temperature before opening (20-25°C / <60% RH). Return unused 3M Petrifilm RYM Plates to pouch. Seal by folding the end of the pouch
over and applying adhesive tape.
To prevent exposure to moisture, do not refrigerate opened pouches.
Store resealed pouches in a cool dry place
(20-25°C / <60% RH) for no longer than 4 weeks. It is recommended that resealed pouches of 3M Petrifilm RYM Plates be stored in a freezer (see
below) if the laboratory temperature exceeds 25ºC (77ºF) and/or the laboratory is located in a region where the relative humidity exceeds 60% (with
the exception of air-conditioned premises).
To store opened pouches in a freezer, place 3M Petrifilm RYM Plates in a sealable container. To remove frozen 3M Petrifilm RYM Plates for use, open
the container, remove the plates that are needed and immediately return remaining plates to the freezer in the sealed container. Allow 3M Petrifilm
RYM Plates to come to room temperature before plating. 3M Petrifilm RYM Plates should not be used past their expiration date. Do not store open
pouches in a freezer with an automatic defrost cycle, as this could damage the 3M Petrifilm RYM Plates due to repeated exposure to moisture.
Do not use 3M Petrifilm RYM Plates that show discoloration. Expiration date and lot number are noted on each package of 3M Petrifilm RYM Plates.
The lot number is also noted on individual 3M Petrifilm RYM Plates.
DISPOSAL
After use, 3M Petrifilm RYM Plates may contain microorganisms that may be a potential biohazard. Follow current industry standards for disposal.
For information on potential biohazards, reference Biosafety in Microbiological and Biomedical Laboratories, 5
th
edition, Section VIII-B: Fungal Agents
or equivalent.
INSTRUCTIONS FOR USE
Follow all Product Instructions carefully. Failure to do so may lead to inaccurate results.
Wear appropriate protective apparel and follow standard good laboratory safety practices (GLP).
1
Sample Preparation
1. Prepare appropriate dilution(s) of the sample as needed.
Use appropriate sterile diluents:
Butterfield’s phosphate buffer (ISO 5541-1), Buffered Peptone Water (ISO), 0.1% peptone water, peptone salt diluent, saline solution (0.85-0.90%),
bisulfite-free letheen broth, or distilled water.
Do not use diluents containing citrate, bisulfite or thiosulfate with 3M Petrifilm RYM Plates;
they can inhibit growth.
If citrate buffer is indicated in the standard procedure, substitute with 0.1% peptone water, warmed to 40-45°C.
See “Specific Instructions for Validated Methods“ for specific requirements.
2. Blend or homogenize sample.
Plating
1. Place the 3M Petrifilm RYM Plate on a flat, level surface.
2. Lift the top film and with the pipette perpendicular dispense 1 mL of sample suspension onto the center of bottom film.
3. Roll the top film down onto the sample.
4. Place the 3M™ Petrifilm™ Flat Spreader (6425) or other flat spreader on the center of the 3M Petrifilm RYM Plate. Press gently on the center of
the spreader to distribute the sample evenly. Spread the inoculum over the entire 3M Petrifilm RYM Plate growth area before the gel is formed. Do
not slide the spreader across the film.
5. Remove the 3M Petrifilm Flat Spreader and leave the 3M Petrifilm RYM Plate undisturbed for at least one minute to permit the gel to form.
Incubation
Incubate 3M Petrifilm RYM Plates at 25°C +/- 1°C or 28°C +/-1°C for 48 +/- 2 hours* in a horizontal position with the clear side up in stacks of no
more than 40.
*If colonies appear faint, allow an additional 12 hours of incubation time for enhanced interpretation. If a 60 hour time-point for interpretation is not
convenient, extending the incubation time to 72 hours is an acceptable alternative.
See “Specific Instructions for Validated Methods“ for specific requirements.
